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Six manno-oligosaccharides were isolated from the partial acid hydrolysate of pronase-treated extracellular glycan (pt-ACE-11) of Absidia cylindrospor by gel filtration on Bio Gel P-2 and descending paper chromatography. The pentasaccharide and the higher molecular weight oligosaccharides significantly inhibited the precipitation reaction of intracellular glycans from Absidia cylindrospora (ACI-B), Mucor hiemalis (MhI-B) and Rhizopus nigricans (RnI-B) with anti-ACE serum. Structural analysis showed that these six oligosaccharides were composed of linear (1·6)-α-linked mannopyranosyl residues.
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