1887

Abstract

The transition from the fermentative mode of growth on glucose to the oxidative mode of growth on ethanol by is accompanied by many changes collectively known as catabolite derepression. Catabolite derepression caused a progressive increase in the total content of cellular sterols, amounting to a fourfold increase after 8 h; the additional sterols existed almost entirely as esters of monoenoic fatty acids. The increase in sterol content could be explained by the large increase in the activity of β-hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34), but a secondary factor in sterol accumulation may have been the slower rate of cell growth during ethanol oxidation. Subsequent anaerobic growth of derepressed cells resulted in the hydrolysis of steryl esters to free sterols, and a progressive depletion of cellular sterol content.

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/content/journal/micro/10.1099/00221287-111-2-343
1979-04-01
2021-10-16
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References

  1. ANDREASON A.A., STIER T.J.B. 1953; Anaerobic nutrition of Saccharomyces cerevisiae : (1) ergosterol requirement for growth in a defined medium.. Journal of Cellular and Comparative Physiology 41:23–26
    [Google Scholar]
  2. ARIES V., KIRSOP B.H. 1978; Sterol biosynthesis by strains of Saccharomyces cerevisiae in the presence and absence of dissolved oxygen.. Journal of the Institute of Brewing 84:118–122
    [Google Scholar]
  3. ASTIN A.M., HASLAM J.M., WOODS R.A. 1977; The manipulation of cellular cytochrome and lipid composition in a haem mutant of Saccharomyces cerevisiae.. Biochemical Journal 166:275–285
    [Google Scholar]
  4. BAILEY R. B., PARKS L.W. 1975; Yeast sterol esters and their relationship to the growth of yeast.. Journal of Bacteriology 124:606–612
    [Google Scholar]
  5. BROWN C.M., JOHNSON B. 1970; Influence of the concentration of glucose and galactose on the physiology of Saccharomyces cerevisiae in continuous culture.. Journal of General Microbiology 64:279–287
    [Google Scholar]
  6. COOPER J.D.H. 1971; Determination of blood ethanol by gas chromatography,. Clinica chimica acta 33:483–485
    [Google Scholar]
  7. DEMEL R.A., DE KRUYFF B. 1976; The function of sterols in membranes.. Biochimica et biophysics acta 457:109–132
    [Google Scholar]
  8. GAL’TSOVA R.O., LYAPUNOVA T.S. 1968; Effect of ethanol on biosynthesis of sterols by yeast.. Microbiology 37:555–557
    [Google Scholar]
  9. HOSSACK J.A., BELK O.M., ROSE A.H. 1977; Environmentally induced changes in the neutral lipids and intracellular vesicles of Saccharomyces cerevisiae and Kluyveromyces fragilis. Archives of Microbiology 114:137–142
    [Google Scholar]
  10. JOHNSON B., NELSON S.J., BROWN C.M. 1972; Influence of glucose composition on the physiology and lipid composition of some yeasts.. Antonie van Leeuwenhoek 38:129–136
    [Google Scholar]
  11. LINNANE A.W., HASLAM J.M. 1970; The biogenesis of mitochondria in Saccharomyces cerevisiae.. Current Topics in Cellular Regulation 2:101–172
    [Google Scholar]
  12. LOWRY O.H., ROSEBROUGH N.J., FARR A.L., RANDALL R.J. 1951; Protein measurement with the Folin phenol reagent.. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. MADYASTHA P.B., PARKS L.W. 1969; The effect of cultural conditions on the ergosterol ester components of yeast.. Biochimica et biophysics acta 176:858–862
    [Google Scholar]
  14. MEITES L., MEITE T. 1948; Removal of oxygen from gas streams.. Analytical Chemistry 20:984–985
    [Google Scholar]
  15. NAGAI J., KAWAMURA S., KATSUKI H. 1977; Occurrence of fatty acid esters of sterol intermediates in ergosterol synthesis by yeast during respiratory adaption.. Journal of Biochemistry 81:1665–1673
    [Google Scholar]
  16. NURMINEN T., KONTTINEN K., SUOMALAINEN H. 1975; Neutral lipids in the cells and cell envelope fractions of aerobic bakers yeast and anaerobic brewers yeast.. Chemistry and Physics of Lipid,, 14:15–32
    [Google Scholar]
  17. PRINGLE J.R. Methods for avoiding .proteolytic artefacts in studies of enzymes and other proteins from yeast.. In Prescott. D.M. Methods in Cell Biology, New York: Academic Press.; vol.12 pp. 149–184 1975
    [Google Scholar]
  18. PROUDLOCK J.W., HASLAM J.M., LINNANE A.W. 1971; Biogenesis of mitochondria. 19: Effects of unsaturated fatty acid depletion on the lipid composition and energy metabolism of a fatty acid desaturase mutant of Saccharomyces cerevisiae.. Journal OJ Bioenergetics, 2:327–349
    [Google Scholar]
  19. QURESHI N., DUGAN R.E., NIMMANNIT S., Wu, W.-M., PORTER J.W. 1976; Purification of ,8-hydroxy-,8-methylglutaryl-coenzyme A reductase in yeas. Biochemistry 15:4185–4190
    [Google Scholar]
  20. ROGERS P.J., YUE S.B., STEWART P.R. 1974; Effects of oxygen tension and glucose repression on mitochondria1 protein synthesis in continuous cultures of Saccharomyces cerevisiae.. Journal of Bacteriology 118:523–533
    [Google Scholar]
  21. ROUSER G., YAMAMOTO A., KRITCHEVSKY G. 1971; Cellular membranes : structures and regulation of lipid class composition, species differences, change with age and variations in some pathological states.. Archives of Internal Medicine 127:1105–1121
    [Google Scholar]
  22. SAHEKI T., HOLZER H. 1975; Proteolytic activities in yeast.. Biochimicaetbiophysicaact 384:203–214
    [Google Scholar]
  23. SCHLOTZHAUER P.F., ELLINGTON J.J., SCHEPARTZ A.I. 1977; Thin layer chromatographic procedure for class separation of plant neutral lipids.. Lipids 12:239–241
    [Google Scholar]
  24. SORBUS M.T., HOLMLUND C.E. 1976; Extraction of lipids from yeast. Lipids 11:341–348
    [Google Scholar]
  25. STARR P.R., PARK L.W. 1962; Some factors affecting sterol formation in Saccharomyces cerevisiae.. Journal of Bacteriology 83:1042–1046
    [Google Scholar]
  26. TAKETANI S., NAGAI J., KATSUKI H. 1978; Quantitative aspects of free and esterified sterols in Saccharomyces cerevisiae under various conditions.. Biochimica et biophysica acta 528:416–423
    [Google Scholar]
  27. THOMPSON E.D., BAILEY R.B., PARKS L.W. 1974; Subcellular location of S-adenosylmethionine: AZ4-stero1m ethyltransferase in Saccharomyces cerevisiae.. Biochimica et biophysica acfa 334:116–126
    [Google Scholar]
  28. WALLACE P.G., HUANG M., LINNANE A.W. 1968; Influence of medium composition on the cytology of anaerobically grown Saccharomyces cerevisiae.. Journal of Cell Biology 37:207–220
    [Google Scholar]
  29. WHEELER G.E., ROSE A.H. 1973; Location and properties of an esterase activity in Saccharomyces cerevisiae.. Journal of General Microbiology 74:189–192
    [Google Scholar]
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