Summary: The isolation and characterization of 29 new germination (Ger) mutants of 168 is described. These were classified, along with previously described mutants, into seven groups according to map location. The mutations in 26 GerA mutants mapped between and detailed mapping of two of these has located them very close to . These mutants were deficient in germination in alanine, but responded to the germinative combination of asparagine, glucose, fructose and KCl. One GerB mutant mapped on the origin-proximal side of ; it was normal in germination in alanine, but deficient in germination in a mixture of asparagine, glucose, fructose and KCl. Two GerC mutants were linked to , but were separable from a temperature-sensitive growth deficiency mapping between and . The GerC mutants had a similar germination phenotype to the GerA mutants. Three GerD mutants did not germinate in either of the above germinants or in Penassay Broth. They were located on the side of distal to . The GerE mutant, which did not germinate in any of the three germinants, was located very close to and possessed an altered spore coat. The two GerF mutants were defective in germination in all three germinants and mapped on the origin proximal-side of , but much closer to than did the GerB mutant. A phosphoglycerate kinase-negative mutant altered in germination mapped between and (GerG). These mutants have established a minimum of seven locations important to germination, and will be useful in the development and appraisal of theories of spore germination.


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