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Volume 110, Issue 1

Iron Transport in : Uptake of Iron from Ferriexochelin Free

Abstract

Summary: Exochelins are a group of extracellular iron chelators produced by mycobacteria. Iron uptake by washed suspensions of iron-deficiently grown from Fe(III)-exochelin fractions (at about 1 μ) was greatest from the fractions containing the compounds that naturally predominate in culture filtrates. Uptake from the major fraction, as well as from combined exochelins, had a of about 6 μ and was unaffected by the presence of a large excess of desferriexochelin; it was inhibited by more than 90% by electron transport inhibitors, uncouplers of oxidative phosphorylation, thiol reagents and by anaero-biosis and low temperature. Uptake of iron from Fe-salicylate, which is mediated by myco-bactin, was insensitive to these inhibitors and a 10-fold excess of ferric salicylate did not inhibit Fe-exochelin uptake. Thus mycobactin is probably not involved in the transport of iron from ferriexochelin at physiological concentrations. The rate of uptake of iron from Fe-exochelin into iron-sufficiently grown cells, which contain less than 0·05% of the concentration of mycobactin found in iron-deficiently grown cells, was only slightly lower than the rate of uptake into iron-deficiently grown cells.

Uptake of ferri[H]exochelin, which could only be carried out at high and probably non-physiological concentrations (about 60 μ), was also extremely sensitive to metabolic inhibitors suggesting that the whole complex was being transported. At these high external concentrations of ferriexochelin a second, non-saturable, inhibitor-insensitive iron uptake process occurred. This process was inhibited in iron-deficiently grown cells by ferric salicylate and may therefore involve mycobactin. A similar but not identical second system which was not sensitive to ferric salicylate was found in iron-sufficiently grown cells; this might indicate yet another pathway of iron uptake from ferriexochelin.

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© Society for General Microbiology 1979
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1979-01-01
2024-04-26
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Iron Transport in Mycobacterium smegmatis: Uptake of Iron from Ferriexochelin
Microbiology 110, 193 (1979); https://doi.org/10.1099/00221287-110-1-193
/content/journal/micro/10.1099/00221287-110-1-193
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