1887

Abstract

SUMMARY: S has an enzyme system which splits pyruvic oxime. This permitted the use of hydroxylamine as non-enzymic acyl acceptor in assaying pyruvate oxidation enzymes in crude extracts. Removal of thioctic acid from crude preparations of tetrahymena pyruvic oxidase by alumina treatment resulted in decreased acyl group formation, but did not affect the rate of oxidation in the presence of ferricyanide. Purification of the oxidase removed both the oxime-splitting system and the enzyme which is necessary for removal of thioctic acid from enzymes by the alumina procedure.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-11-2-300
1954-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/11/2/mic-11-2-300.html?itemId=/content/journal/micro/10.1099/00221287-11-2-300&mimeType=html&fmt=ahah

References

  1. Calvin M. 1953; The quantum conversion in photosynthesis. Chem. Engng News 31:1735
    [Google Scholar]
  2. Chou T. C., Lipmann F. 1952; Separation of acetyl transfer enzymes in pigeon liver extract. J. biol. Chem 196:89
    [Google Scholar]
  3. Gunsalus I. C., Dolin M. I., Struglia L. 1952; Pyruvic acid metabolism. III. A manometric assay for pyruvate oxidation factor. J. biol. Chem 194:849
    [Google Scholar]
  4. Jagannathan Y., Schweet R. S. 1952; Pyruvic oxidase of pigeon breast muscle. I. Purification and properties of the enzyme. J. biol. Chem 196:551
    [Google Scholar]
  5. Kalckar H. M. 1947; Differential spectrophotometry of purine compounds by means of specific enzymes. III. Studies on the enzymes of purine metabolism. J. biol. Chem 167:461
    [Google Scholar]
  6. Korkes S., Stern J. R., Gunsalus I. C., Ochoa S. 1950; Enzymatic synthesis of citrate from pyruvate and oxaloacetate. Nature; Lond: 166439
    [Google Scholar]
  7. Lipmann F., Tuttle L. C. 1945a; A specific micromethod for the determination of acylphosphates. J. biol. Chem 159:21
    [Google Scholar]
  8. Lipmann F., Tuttle L. C. 1945b; The detection of carboxyl groups with hydroxylamine as interceptor. J. biol. Chem 161:415
    [Google Scholar]
  9. Meister A. 1952 Crystalline lactic dehydrogenase, in Biochem. Prep Snell E. E. ed 218 London: Chapman and Hall;
    [Google Scholar]
  10. Mehler A. H., Kornberg A., Grisolia S., Ochoa S. 1948; The enzymatic mechanisms of oxidation-reductions between malate or isocitrate and pyruvate. J. biol. Chem 174:961
    [Google Scholar]
  11. Reed L. J., DeBusk B. 1953; Mechanism of enzymatic oxidative decarboxylation of pyruvate. J. Amer. chem. Soc 75:1261
    [Google Scholar]
  12. Robertson W. 1942; The preparation of sodium pyruvate. Science 96:93
    [Google Scholar]
  13. Seaman G. R. 1952; Role of protogen in the oxidation of pyruvic acid. Proc. Soc. Exp. Biol., N.Y 80:308
    [Google Scholar]
  14. Seaman G. R. 1953; Role of thioctic acid in the transfer of acyl groups. Proc. Soc. Exp. Biol., N.Y 82:184
    [Google Scholar]
  15. Seaman G. R. 1954; Participation of thioctic acid in the acetate-activating reaction. J. Amer. chem. Soc 76:1712
    [Google Scholar]
  16. Stadtman E. R. 1952; The purification and properties of phosphotransacetylase. J. biol. Chem 196:527
    [Google Scholar]
  17. Stumpf P. K., Zarudnaya K., Green D. E. 1947; Pyruvic and α-ketoglutaric oxidase of animal tissue. J. biol. Chem 167:817
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-11-2-300
Loading
/content/journal/micro/10.1099/00221287-11-2-300
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error