1887

Abstract

Paper chromatographic methods were used to determine the amino acids in unhydrolysed corn steep liquor and to study the uptake and synthesis of amino acids by . Of the eleven amino acids found in corn steep liquor all were taken up by the organism prior to the main period of penicillin formation. Only traces of ninhydrin-reacting material were present in the later samples of medium.

Glutamine, in addition to the eleven amino acids detected in corn steep liquors, was found in mycelium grown on medium containing corn steep liquor. Mycelium from grown on medium containing inorganic nitrogen as sole nitrogen source showed the presence of the same twelve amino acids.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-11-2-191
1954-10-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/micro/11/2/mic-11-2-191.html?itemId=/content/journal/micro/10.1099/00221287-11-2-191&mimeType=html&fmt=ahah

References

  1. Bentley H. R., Whitehead J. K. 1950; Water-miscible solvents in the separation of amino-acids by paper chromatography. Biochem. J 46:341
    [Google Scholar]
  2. Block R. J., Bolling D. 1951 The Amino Acid Composition of Proteins and Foods Springfield Ill: C. C. Thomas;
    [Google Scholar]
  3. Bolling D., Sober H. A., Block R. J. 1949; Quantitative separation and determination of small amounts of histidine and tyrosine employing paper chromatography. Fed. Proc 8:185
    [Google Scholar]
  4. Cardinal E. Y., Hedrick L. R. 1948; Microbiological assay of com steep liquor for amino acid content. J. biol. Chem 172:609
    [Google Scholar]
  5. Koffler H., Emerson R. L., Perlman D., Burris R. H. 1945; Chemical changes in submerged penicillin fermentations. J. Bact 50:517
    [Google Scholar]
  6. Lumb M., Fawcett R. 1951; Improvements in experimental fermenters. J. appl. Chem 1:S94
    [Google Scholar]
  7. Narasimha Rao P. L., Venkataraman R. 1952; Nitrogen metabolism of Penicillium chrysogenum Q-176. Experientia 8:350
    [Google Scholar]
  8. Proom H., Woiwod A. J. 1949; The examination, by partition paper chromatography, of the nitrogen metabolism of bacteria. J. gen. Microbiol 3:319
    [Google Scholar]
  9. Rolinson G. N., Lumb M. 1953; The oxidation of lard oil by Penicillium chrysogenum. J. gen. Microbiol 9:385
    [Google Scholar]
  10. Smith A. M., Agiza A. H. 1951; The amino acids of several grassland species, cereals and bracken. J. Sci. Fd Agric 2:503
    [Google Scholar]
  11. Stefaniak J. J., Gailey F. B., Jarvis F. G., Johnson M. J. 1946; The effect of environmental conditions on penicillin fermentations with Penicillium chrysogenum X-1612. J. Bact 52:119
    [Google Scholar]
  12. Wolf F. T. 1948; The amino acid metabolism of Penicillium chrysogenum Q-176. Arch. Biochem l6:143
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-11-2-191
Loading
/content/journal/micro/10.1099/00221287-11-2-191
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error