1887

Abstract

SUMMARY: The investigation of the metabolism of thiocyanate by pure cultures of was complicated by the fact that thiocyanate serves both as source of energy and as source of carbon and nitrogen. It was, therefore, difficult to separate oxidation from carbon dioxide fixation. The equation which best represented the resultant of these two processes was:

2KCNS + 5HO + 3O = KSO + (NH)SO + CO + (CHO).

Thiocyanate was first hydrolysed to cyanate and sulphide. Cyanate was further hydrolysed to carbon dioxide and ammonia and sulphide was oxidized to sulphate. The oxidation of sulphide approximated to the equation:NaS + 2O = NaSO. Gas uptakes were lower than would be expected from this equation, although special precautions were taken to prevent loss of sulphide.

Thiosulphate was oxidized to sulphate according to the equation:

NaSO + HO + 2O = NaSO + HSO.

Fixation of carbon dioxide after the oxidation of thiosulphate was shown, but the efficiency of energy utilization appeared lower than during the oxidation of thiocyanate.

Thioacetamide and thioacetic acid were oxidized to sulphate but this was believed to be preceded by a chemical breakdown to sulphide. Sulphite, metabisulphite, dithionite, dithionate, tetrathionate, trithionate, thiourea and cysteine were tested; no evidence was obtained of their utilization.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-11-2-139
1954-10-01
2021-07-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/11/2/mic-11-2-139.html?itemId=/content/journal/micro/10.1099/00221287-11-2-139&mimeType=html&fmt=ahah

References

  1. Gleen H. 1949; The metabolism of thiocyanate oxidising bacteria in soil. 1st Int. Congr. Biochem 514:
    [Google Scholar]
  2. Happold F. C., Key A. 1934; Institution of Gas Engineers.4th Report of the Liquor Effluents and Ammonia Subcommittee.
    [Google Scholar]
  3. Happold F. C., Key A. 1937; The bacterial purification of gas works liquors. 11. The biological oxidation of ammonium thiocyanate. Biochem. J 31:1323
    [Google Scholar]
  4. Happold F. C., Johnstone K. I., Rogers H. J. 1952; An examination of Bacterium tkiocyanoxidans. Nature; Lond: 169332
    [Google Scholar]
  5. Happold F. C., Johnstone K. I., Rogers H. J., Youatt J. B. 1954; Isolation and characteristics of an organism oxidizing thiocyanate. J. gen. Microbiol 10:261
    [Google Scholar]
  6. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. Brit. J. exp. Path 32:97
    [Google Scholar]
  7. Kurtenacker A., Matejka J. 1930; Über die Darstellung von Tetra- und Trithionat aus Thiosulfat und Schwefliger Säure. Z. anorg. Chem 193:367
    [Google Scholar]
  8. Pomeroy R. 1936; An improved method for the estimation of sulphides. Waterwks & Sewerage 83:279
    [Google Scholar]
  9. Vogler K. G. 1942; Studies in the metabolism of autotrophic bacteria. II. The nature of the chemosynthetic reaction. J. gen. Physiol 26:89
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-11-2-139
Loading
/content/journal/micro/10.1099/00221287-11-2-139
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error