1887

Abstract

SUMMARY: Strains of and , including mucoid capsulate, mucoid non-capsulate, smooth and rough variant forms, were grown on defined agar media. The polysaccharide content of the cultures was estimated by the anthrone method and related to the amount of growth as measured by the non-dialysable nitrogen content. Polysaccharide production, as measured by the polysaccharide: nitrogen ratio, was increased in all strains when growth was limited by a restricted supply of nitrogen source so that added carbohydrate remained in relative excess. A low incubation temperature, 15–20° instead of 35°, influenced polysaccharide production differently in the various strains and species; thus it greatly increased production in strains, but decreased it in strains grown on certain media. The various strains showed considerable differences in the amount of polysaccharide production and in the distribution of the polysaccharide between the different possible sites of accumulation: i.e. intracellular, capsular and as loose slime. The mucoid strains produced the largest amounts of polysaccharide, most of which was present extracellularly as capsules or slime; the smooth strains produced much smaller amounts, almost entirely intracellular; a rough strain produced least of all. The antigenic constitution of certain strains was examined, particularly that of a mucoid capsulate and mucoid non-capsulate and non-mucoid smooth variants derived from it. The designation of such morphological and immunological variants is discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-11-1-59
1954-08-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/11/1/mic-11-1-59.html?itemId=/content/journal/micro/10.1099/00221287-11-1-59&mimeType=html&fmt=ahah

References

  1. Austrian R. 1953; Morphologic variation in pneumococcus. I. An analysis of the bases for morphologic variation in pneumococcus and description of a hitherto undefined morphologic variant. J. exp. Med 98:21
    [Google Scholar]
  2. Beiser S. M., Davis B. D. 1953; Mucoid mutants of Escherichia coli. Fed. Proc 12:436
    [Google Scholar]
  3. Bernheimer A. W. 1953; Synthesis of type III pneumococcus polysaccharide by suspensions of resting cells. J. exp. Med 97:591
    [Google Scholar]
  4. Boivin A., Mesrobeanu L., Magheru G., Magheru A. 1936; Sur les rapports existants entre l’aspect des colonies données par les colibacilles et la présence ou l’absence d’antigène somatique ‘complet’ dans ces bactéries. C.R. Soc. Biol., Paris 121:169
    [Google Scholar]
  5. Duguid J. P. 1948; The influence of cultural conditions on the morphology of Bacterium aerogenes with reference to nuclear bodies and capsule size. J. Path. Bact 60:265
    [Google Scholar]
  6. Duguid J. P. 1951; The demonstration of bacterial capsules and slime. J. Path. Bact 63:673
    [Google Scholar]
  7. Duguid J. P., Wilkinson J. F. 1953; The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. J. gen. Microbiol 9:174
    [Google Scholar]
  8. Edwards P. R., Fife M. A. 1952; Capsule types of Klebsiella. J. infect. Dis 91:92
    [Google Scholar]
  9. Edmunds P. N. 1954; Further Klebsiella capsule types. J. infect. Dis 94:65
    [Google Scholar]
  10. Hotchkiss R. D. 1948; A microchemical reaction resulting in the staining of polysaccharide structures in fixed tissue preparations. Arch. Biochem 16:131
    [Google Scholar]
  11. Kauffmann F. 1947; The serology of the coli group. J. Immunol 57:71
    [Google Scholar]
  12. Kauffmann F. 1949; On the serology of the Klebsiella group. Acta path. microbiol. scand 26:381
    [Google Scholar]
  13. Lankford C. E., Hoyo H., Lutteringer J. F. 1951; Intracellular polysaccharide of Enterobacteriaceae. J. Bact 62:621
    [Google Scholar]
  14. Macpherson I. A., Wilkinson J. F., Swain R. H. A. 1953; The effect of Klebsiella aerogenes and Klebsiella cloacae polysaccharides on haemagglutination by and multiplication of the influenza group of viruses. Brit. J. exp. Path 34:603
    [Google Scholar]
  15. Morgan H. R., Beckwith T. D. 1939; Mucoid dissociation in the colontyphoid-salmonella group. J. infect. Dis 65:113
    [Google Scholar]
  16. Pickett M. J., Cabelli V. J. 1953; The precipitating antigens of Friedländer’s bacillus. J. gen. Microbiol 9:249
    [Google Scholar]
  17. Wilson G. S. 1935; The bacteriological grading of milk. Spec. Rep. Ser. med. Res. Coun., Lond206 p. 156
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-11-1-59
Loading
/content/journal/micro/10.1099/00221287-11-1-59
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error