1887

Abstract

Urealytic activity of the cytoplasmic fraction of prepared by digitonin lysis was assayed in a simple buffer system (HEPES plus EDTA) by measuring the release of CO from [C]urea. The of this preparation agreed with our previous observations of the same activity measured in a more complex reaction mixture. The substrate concentration at which maximum velocity occurred was approximately 20 m. The activity was sensitive to heavy metals and inhibitors which react with sulphydryl groups such as -ethylmaleimide and -chloromercuribenzoate. It was not inhibited by Ca or Mg or by the reaction products, ammonia and carbon dioxide.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-108-2-221
1978-10-01
2024-11-07
Loading full text...

Full text loading...

/deliver/fulltext/micro/108/2/mic-108-2-221.html?itemId=/content/journal/micro/10.1099/00221287-108-2-221&mimeType=html&fmt=ahah

References

  1. Delisle G. J. 1977; Multiple forms of urease in cytoplasmic fractions of Ureaplasma urealyticum . Journal of Bacteriology 130:1390–1392
    [Google Scholar]
  2. Furness G., Coles R. S. 1975; The urea requirement and urease production of some human species of T-mycoplasmas. Proceedings of the Society for Experimental Biology and Medicine 150807–809
    [Google Scholar]
  3. Kalb V. F., Bernlohr R. W. 1975; A 260/230 method for the determination of protein concentration in the presence of nucleic acids. Federation Proceedings 343
    [Google Scholar]
  4. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  5. Masover G. K., Hayflick L. 1973; Growth of a T-strain mycoplasma in medium without added urea. Annals of the New York Academy of Sciences 225:118–130
    [Google Scholar]
  6. Masover G. K., Benson J. R., Hayflick L. 1974; Growth of T-strain mycoplasmas in medium without added urea; effect of trace amounts of urea and of a urease inhibitor. Journal of Bacteriology 117:765–774
    [Google Scholar]
  7. Masover G. K., Mischak R. P., Hayflick L. 1975; Some effects of growth medium composition on the antigenic character of a T-strain mycoplasma. Infection and Immunity 11:530–539
    [Google Scholar]
  8. Masover G. K., Sawyer J. E., Hayflick L. 1976; Urea-hydrolyzing activity of a T-strain mycoplasma : Ureaplasma urealyticum. Journal of Bacteriology 125:581–587
    [Google Scholar]
  9. Masover G. K., Razin S., Hayflick L. 1977a; Effects of carbon dioxide, urea and ammonia on growth of Ureaplasma urealyticum (T-strain mycoplasma). Journal of Bacteriology 130:292–296
    [Google Scholar]
  10. Masover G. K., Razin S., Hayflick L. 1977b; Localization of enzymes in Ureaplasma urealyticum (T-strain mycoplasma). Journal of Bacteriology 130:297–302
    [Google Scholar]
  11. Razin S., Rottem S. 1976; Techniques for the manipulation of mycoplasma membranes. Biochemical Analysis of Membranes3–26 Maddy A. H. London: Chapman & Hall;
    [Google Scholar]
  12. Roon A. J., Levenberg B. 1970; CO2 fixation and the involvement of allophanate in the biotin-enzyme catalyzed cleavage of urea. Journal of Biological Chemistry 245:4593–4595
    [Google Scholar]
  13. Rottem S., Razin S. 1972; Isolation of mycoplasma membranes by digitonin. Journal of Bacteriology 110:669–705
    [Google Scholar]
  14. Seneca H., Peer P., Nally R. 1972; Microbial urease. Nature London: 1931106–1107
    [Google Scholar]
  15. Shepard M. C., Lunceford C. D., Ford D. K., Purcell R. H., Taylor-Robinson D., Razin S., Black F. T. 1974; Ureaplasma urealyticum gen.nov., sp.nov. ; proposed nomenclature for the human T (T-strain) mycoplasmas. International Journal of Systematic Bacteriology 24:160–171
    [Google Scholar]
  16. Sumner J. B. 1951; Urease. The Enzymes 12873–892 Sumner J. B., Myrbach K. New York: Academic Press;
    [Google Scholar]
  17. Vinther O. 1976; Localization of urease activity in Ureaplasma urealyticum cells. Proceedings of the Society for General Microbiology 3159
    [Google Scholar]
  18. Webb J. L. 1966 Enzyme and Metabolic Inhibitors II343–345 III768–870 New York: Academic Press;
    [Google Scholar]
/content/journal/micro/10.1099/00221287-108-2-221
Loading
/content/journal/micro/10.1099/00221287-108-2-221
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error