1887

Abstract

Genetic relationships among bacterial endosymbionts of assigned to the genus Preer . 1974 were determined using DNA-DNA hybridization techniques. It was shown that mu, nu and pi endosymbionts are closely related to each other, but not to any of the strains of kappa endosymbionts tested, and that kappa consists of at least three distinct and genetically diverse groups. Therefore, it is proposed that be split into two genera, and , depending upon the ability or lack of ability, respectively, to produce cells containing R bodies. Strains were screened for covalently closed, circular DNA by ethidium bromide/CsCl centrifugation, but only pi and hump-killer kappa particles were found to contain this form of DNA.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-108-2-181
1978-10-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/108/2/mic-108-2-181.html?itemId=/content/journal/micro/10.1099/00221287-108-2-181&mimeType=html&fmt=ahah

References

  1. Anderson T. F., Preer J. R., Preer L. B., Bray M. 1964; Studies on killing particles from Paramecium : the structure of refractile bodies from kappa particles. Journal de Microscopie 3:395–402
    [Google Scholar]
  2. Brenner D. J. 1973; Deoxyribonucleic acid reassociation in the taxonomy of enteric bacteria.. International Journal of Systematic Bacteriology 21:298–307
    [Google Scholar]
  3. Dilts J. A. 1976; Covalently closed, circular DNA in kappa endosymbionts of Paramecium . Genetical Research 27:161–170
    [Google Scholar]
  4. Dilts J. A. 1977; Chromosomal and extra-chromosomal deoxyribonucleic acid from four bacterial endosymbionts derived from stock 51 of Paramecium tetraurelia. . Journal of Bacteriology 129:888–894
    [Google Scholar]
  5. Mueller J. A. 1963; Separation of kappa particles with infective activity from those with killing activity and identification of the infective particles in Paramecium aurelia . Experimental Cell Research 30:492–508
    [Google Scholar]
  6. Preer J. R. Jr, Preer L. B. 1967; Virus-like bodies in killer paramecia. Proceedings of the National Academy of Sciences of the United States of America 581774–1781
    [Google Scholar]
  7. Preer J. R. Jr, Preer L. B., Rudman B. M., Jurand A. 1971; Isolation and composition of bacteriophage-like particles from kappa of killer paramecia. Molecular and General Genetics 111202–208
    [Google Scholar]
  8. Preer J. R. Jr, Preer L. B., Jurand A. 1974; Kappa and other endosymbionts in Paramecium aurelia . Bacteriological Reviews 38:113–163
    [Google Scholar]
  9. Preer L. B., Jurand A., Preer J. R. Jr, Rudman B. M. 1972; The classes of kappa in Paramecium aurelia . Journal of Cell Science 11:581–600
    [Google Scholar]
  10. Preer L. B., Rudman B. M., Preer J. R. Jr, Jurand A. 1974; Induction of R bodies by ultraviolet light in killer paramecia. Journal of General Microbiology 80:209–215
    [Google Scholar]
  11. Quackenbush R. L. 1977a; Phylogenetic relationships of bacterial endosymbionts of Paramecium aurelia: polynucleotide sequence relationships of 51 kappa and its mutants. Journal of Bacteriology 129:895–900
    [Google Scholar]
  12. Quackenbush R. L. 1977b; Genetic relationships among bacterial endosymbionts of Paramecium aurelia. Ph.D. thesis Indiana University Bloomington, Indiana, U.S.A:
    [Google Scholar]
  13. Singler-Bastiaans M. J. 1975; Studies with killer paramecia. Ph.D. thesis Indiana University Bloomington, Indiana, U.S.A:
    [Google Scholar]
  14. Smith J. E. 1961; Purification of kappa particles of Paramecium aurelia stock 51.. American Zoologist 1:390
    [Google Scholar]
  15. Soldo A. T., Godoy G. A., Van Wagtendonk W. J. 1966; Growth of particle-bearing and particle-free Paramecium aurelia in axenic culture.. Journal of Protozoology 13:492–497
    [Google Scholar]
  16. Sonneborn T. M. 1970 Methods in Cell Physiology 4:242–339
    [Google Scholar]
  17. Sonneborn T. M. 1975; The Paramecium aurelia complex of fourteen sibling species. Transactions of the American Microscopical Society 94:155–178
    [Google Scholar]
  18. Thompson R., Hughes S. G., Broda P. 1974; Plasmid identification using specific endo-nucleases.. Molecular and General Genetics 133:141–149
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-108-2-181
Loading
/content/journal/micro/10.1099/00221287-108-2-181
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error