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Thiobacillus denitrificans grew exclusively aerobically in thiosulphate-limited chemostat culture at all dissolved oxygen concentrations between 12 and 216 μ m. Nitrate reduction did not occur in aerobic cultures and nitrate and nitrite reductases only reached high levels under complete anaerobiosis. Growth yield was greatest [11.50 g dry wt (mol thiosulphate oxidized)–1] at the lowest dissolved oxygen concentration (12 μ m) and decreased at higher dissolved oxygen concentrations, indicating oxygen to be a growth-inhibitory substrate; the anaerobic yield was only 77 % of the maximum aerobic yield (all tested at a dilution rate of 0.08 h–1) in agreement with thermodynamic calculations. Low activities of thiosulphate-oxidizing enzyme and sulphur-oxidizing enzyme were detected in aerobic cultures, but activities were even lower in anaerobic cultures. Efficient energy coupling mechanisms with respect to sulphur and thiosulphate oxidations are indicated.
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