1887

Abstract

Specialized transduction by phage 16-3 of one of the markers of is described. The frequency of transduction was 10 to 10 per plaque-forming unit. About two-thirds of the transductants were heterogenotes, unstable for the marker, and yielded high frequency transducing lysates (10 per plaque-forming unit). In phage lysates, plaque-forming transducing particles were directly demonstrated while non-plaque-forming particles were only detected indirectly via complementation of defective lysogenic transductants. In the defective transductants, the two distal ends of the vegetative phage map were lacking. By plasmid transfer, the attachment site of phage 16-3 was located close to the and markers. Transduction of the marker could not be demonstrated.

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1978-06-01
2021-08-04
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References

  1. Arber W. 1958; Transduction des caracteres gal par le bacteriophage lambda. Archives des sciences, Geneva 11:259–338
    [Google Scholar]
  2. Balassa G. 1963; Genetic transformation of Rhizobium: a review of the work of R. Balassa. Bacteriological Reviews 27:228–241
    [Google Scholar]
  3. Beringer J. E., Hopwood D. A. 1976; Chromosomal recombination and mapping in Rhizobium leguminosarum. Nature London: 264:291–293
    [Google Scholar]
  4. Coetzee J. N. 1974; High frequency transduction of kanamycin resistance in Proteus mirabilis. Journal of General Microbiology 84:285–296
    [Google Scholar]
  5. Iijima T. 1966; Specialized transduction of the galactose markers by a temperate phage 0170 in Escherichia coli k12. Japanese Journal of Genetics 41:121–130
    [Google Scholar]
  6. Kondorosi A., Barabas I., Svab Z., Orosz L., Sik T., Hotchkiss R. D. 1973; Evidence for common genetic determinants of nitrogenase and nitrate reductase in Rhizobium meliloti. Nature, New Biology 246:153–154
    [Google Scholar]
  7. Kondorosi A., Orosz L., Svab Z., Sik T. 1974; Genetic studies on Rhizobiophage 16-3. II. Helper-induced transfection. Molecular and General Genetics 132:153–163
    [Google Scholar]
  8. Kondorosi A., Svab Z., Kiss G. B., Dixon R. A. 1977a; Ammonia assimilation and nitrogen fixation in Rhizobium meliloti. Molecular and General Genetics 151:221–226
    [Google Scholar]
  9. Kondorosi A., Kiss G. B., Forrai T., Yincze E., Banfalvi Z. 1977b; Circular linkage map of Rhizobium meliloti chromosome. Nature, London 268:525–527
    [Google Scholar]
  10. Kowalski M. 1967; Transduction in Rhizobium meliloti. Acta microbiologica polonica 16:7–12
    [Google Scholar]
  11. Luria S. E., Adams J. N., Ting R. C. 1960; Transduction of lactose-utilizing ability among strains of E. coli and S. dysenteriae and the properties of the transducing phage particles. Virology 12:348–390
    [Google Scholar]
  12. Matsushiro A. 1963; Specialized transduction of tryptophan markers in Escherichia coli k12 by bacteriophage 080. Virology 19:475–482
    [Google Scholar]
  13. Matsushiro A., Sato K., Kida S. 1964; Characteristics of the transducing elements of bacteriophage 080. Virology 23:299–306
    [Google Scholar]
  14. Meade H. N., Signer E. R. 1977; Genetic mapping of Rhizobium meliloti. Proceedings of the National Academy of Sciences of the United States of America 74:2076–2078
    [Google Scholar]
  15. Morse M. L., Lederberg E. M., Lederberg J. 1956; Transduction in Escherichia coli k12. Genetics 41:142–156
    [Google Scholar]
  16. Orosz L., Sik T. 1970; Genetic mapping of Rhizobiophage 16-3. Acta microbiologica Acade-miae scientiarium hungaricae 17:185–194
    [Google Scholar]
  17. Orosz L., Svab Z., Kondorosi A., Sik T. 1973; Genetic studies on Rhizobiophage 16-3. I. Genes and functions on the chromosome. Molecular and General Genetics 125:341–350
    [Google Scholar]
  18. Sik T., Orosz L. 1971; Chemistry and genetics of Rhizobium meliloti phage 16-3. Plant and Soil special volume:57–62
    [Google Scholar]
  19. Smith-Keary P. F. 1966; Restricted transduction by bacteriophage P22 in Salmonella typhimurium. Genetical Research 8:73–82
    [Google Scholar]
  20. Svab Z., Orosz L., Kondorosi A., Sik T. 1973; Study of a specially transducing Rhizobium phage. Acta microbiologica Academiae scientiarium hungaricae 20:24
    [Google Scholar]
  21. Szende K., Ordogh F. 1960; Die Lysogenie von Rhizobium meliloti. Naturwissenschaften 47:404–405
    [Google Scholar]
  22. Takeda Y., Morishita T., Yura T. 1970; Specialized transduction of the galactose genes of Escherichia coli by phase 081. Virology 41:312–355
    [Google Scholar]
  23. Wollman E. L. 1963; Transduction specifique du margueur biotine par le bacteriophage A. Comptes rendus hebdomadaire des seances de VAcademie des sciences 257:4225–4226
    [Google Scholar]
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