1887

Abstract

The pectic enzymes of six strains of pigmented, pectolytic clostridia isolated from potatoes were examined and compared with those strains of and using cup-plate assays. The organisms could be divided into two groups. Group 1 consisted of the potato isolates and the strain of these formed pectic lyase enzymes and pectinesterase but no pectic hydrolase. Group 2 consisted of eight strains of (and one culture labelled ); these formed pectic lyase and pectic hydrolase but no detectable pectinesterase. Viscometric studies and analyses of degradation products of pectate formed by enzymes of four representative strains from group 1 and two strains from group 2 showed that all these clostridia formed endopectate lyase enzymes; studies of one enzyme preparation showed that the pH optimum was about 9·5. In addition, the group 2 organisms formed endopolygalacturonase and probably also exopolygalacturonase.

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1978-01-01
2024-11-11
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