1887

Abstract

The pectic enzymes of six strains of pigmented, pectolytic clostridia isolated from potatoes were examined and compared with those strains of and using cup-plate assays. The organisms could be divided into two groups. Group 1 consisted of the potato isolates and the strain of these formed pectic lyase enzymes and pectinesterase but no pectic hydrolase. Group 2 consisted of eight strains of (and one culture labelled ); these formed pectic lyase and pectic hydrolase but no detectable pectinesterase. Viscometric studies and analyses of degradation products of pectate formed by enzymes of four representative strains from group 1 and two strains from group 2 showed that all these clostridia formed endopectate lyase enzymes; studies of one enzyme preparation showed that the pH optimum was about 9·5. In addition, the group 2 organisms formed endopolygalacturonase and probably also exopolygalacturonase.

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1978-01-01
2021-08-05
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References

  1. Abdel-Fattah A. F., Mabrouk S. S., Edrees M. 1971; Degradation of flax pectin by polygalacturonase of Clostridium felsineum and some aspects of the detection of pectin-methylesterase. Journal of General and Applied Microbiology 17:421–427
    [Google Scholar]
  2. Barnes E. M., Impey C. S., Goldberg H. S. 1966; Methods for the characterization of the Bacteroidaceae. In Identification Methods for Microbiologists part A pp. 51–58 Gibbs B. M., Skinner F. A. Edited by London: Academic Press;
    [Google Scholar]
  3. Bateman D. F., Millar R. L. 1966; Pectic enzymes in tissue degradation. Annual Review of Plant Pathology 4:119–146
    [Google Scholar]
  4. Breed R. S., Murray E. G. D., Smith N. R.editors 1957 Bergey’s Manual of Determinative Bacteriology, 7th edn.. London: Balliere, Tindall & Cox;
    [Google Scholar]
  5. Dingle J., Reid W. W., Solomons G. L. 1953; The enzymic degradation of pectin and other polysaccharides.II. Application of the ‘cup-plate’ assay to the estimation of enzymes. Journal of the Science of Food and Agriculture 4:149–155
    [Google Scholar]
  6. Fogarty W. M., Ward O. P. 1974; Pectinases and pectic polysaccharides. Progress in Industrial Microbiology 13:59–119
    [Google Scholar]
  7. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods in Enzymology 1:138–146
    [Google Scholar]
  8. Hasegawa S., Nagel C. W. 1962; The characterization of an α,β-unsaturated digalacturonic acid. Journal of Biological Chemistry 237:619–621
    [Google Scholar]
  9. Hellinger E. 1947; Clostridium aurantibutyricum(n.sp.): a pink, butyric acid Clostridium. Journal of General Microbiology 1:203–210
    [Google Scholar]
  10. Horikoshi K. 1972; Production of alkaline enzymes by alkalophilic micro-organisms. Part III. Alkaline pectinase of Bacillus No. P-4-N. Agricultural and Biological Chemistry 36:285–293
    [Google Scholar]
  11. Jansen E. F., Macdonnel L. R. 1945; Influence of methoxyl content of pectic substances on the action of polygalacturonase. Archives of Biochemistry 8:97–118
    [Google Scholar]
  12. Kaji A. 1956; Studies of macerating enzyme acting on middle lamella pectin. I. Separation of the macerating enzyme produced by Clostridium felsineum var. sikokianum. Bulletin of the Agricultural Chemical Society of Japan 20:8–12
    [Google Scholar]
  13. Kaji A. 1959; Studies of macerating enzyme acting on middle lamella pectin. IV. Action of macerating enzyme on pectic substances. Bulletin of the Agricultural Chemical Society of Japan 23:131–136
    [Google Scholar]
  14. Kaji A., Anabuki Y. 1955; On the purification and properties of bacterial polygalacturonase. Journal of the Agricultural Chemical Society of Japan 29:775–780
    [Google Scholar]
  15. Kaji A., Tachibana S., Aihara S., Anabuki Y. 1959; Studies on macerating enzyme. V. Separation of pectic enzymes by zone electrophoresis. Technical Bulletin of the Faculty of Agriculture of Kagawa University 11:248–254
    [Google Scholar]
  16. Lund B. M. 1972; Isolation of pectolytic clostridia from potatoes. Journal of Applied Bacteriology 35:609–614
    [Google Scholar]
  17. Lund B. M., Kelman A. 1977; Determination of the potential for development of bacterial soft rot of potatoes. American Potato Journal 54:211–225
    [Google Scholar]
  18. Macmillan J. D., Vaughn R. G. 1964; Purification and properties of a polygalacturonic acid trans-eliminase produced by Clostridium multifermentans. Biochemistry 3:564–572
    [Google Scholar]
  19. Mccomb E. A., Mccready R. M. 1958; Use of the hydroxamic acid reaction for determining pectinesterase activity. Stain Technology 33:129–131
    [Google Scholar]
  20. Moran F., Nasuno S., Starr M. P. 1968; Extracellular and intracellular polygalacturonic acid trans-eliminases of Erwinia carotovora. Archives of Biochemistry and Biophysics 123:298–306
    [Google Scholar]
  21. Nagel C. W., Vaughn R. H. 1962; Comparison of growth and pectolytic enzyme production by Bacillus polymyxa. Journal of Bacteriology 83:1–5
    [Google Scholar]
  22. Nasuno S., Starr M. P. 1966; Polygalacturonase of Erwinia carotovora. Journal of Biological Chemistry 241:5298–5306
    [Google Scholar]
  23. Osman H. G., Abdel-Fattah A. F., Abdel-Samie M. 1969; Bacterial pectolytic enzymes. I. Isolation of Clostridium felsineum from the retting water of Egyptian flax, separation and pectolytic activity of its polygalacturonase. Journal of Chemistry of the UAR 12:543–550
    [Google Scholar]
  24. Roboz E., Barratt R. W., Tatum E. L. 1952; Breakdown of pectic substances by a new enzyme from Neurospora. Journal of Biological Chemistry 195:459–471
    [Google Scholar]
  25. Rodionova N. A., Kapitonova L. S., Feniksova R. B. 1972; Macerating enzymes of Clostridium felsineum. Doklady Akademii Nauk SSSR 207:466–468 Cited in Chemical Abstracts 78, 94421 y(1973)
    [Google Scholar]
  26. Rombouts F. M. 1972 Occurrence and properties of bacterial pectatelyases. Ph.D. thesis, Agricultural University, Wageningen, The Netherlands
    [Google Scholar]
  27. Rombouts F. M., Pilnik W. 1972; Research on pectin depolymerases in the sixties, a literature review. CRC Critical Reviews in Food Technology 3:1–26
    [Google Scholar]
  28. Rudd-Jones D., Dowson W. J. 1950; On the bacteria responsible for soft rot in stored tubers and the reaction of the tuber to invasion by Bacterium carotovorum (Jones) Lehmann & Neumann. Annals of Applied Biology 37:563–569
    [Google Scholar]
  29. Swoboda P. A. T., Lea C. H. 1958; Determination of the peroxide value of edible fats by colorimetric iodometric procedures. Chemistry and Industry1090–1091
    [Google Scholar]
  30. Trevelyan W. E., Procter D. R., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature, London 166:444–445
    [Google Scholar]
  31. Voznyakovskaya YU M., Avrova N. P., Andronikashvili E. D. 1974; Reproduction and synthesis of pectolytic enzymes by Clostridium felsineum on media with various carbon sources. Mikrobiologiya 43:423–427
    [Google Scholar]
  32. Warren L. 1960; Thiobarbituric acid spray reagent for deoxy sugars and sialic acids. Nature, London 186:237
    [Google Scholar]
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