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Volume 103, Issue 1

Regulation of Synthesis of Benzyl Alcohol Dehydrogenase in 8250 Free

Abstract

Specific activity of benzyl alcohol dehydrogenase in carbon-limited continuous cultures was at a maximum at a specific growth rate of 0·2 h, but fell off at lower and higher growth rates. The specific activity in nitrogen-limited cultures was always lower and was inversely proportional to growth rate. There was severe repression of benzyl alcohol dehydrogenase during metabolism of (+)-mandelate or phenylglyoxylate in batch cultures. Synthesis of benzyl alcohol dehydrogenase was followed in experiments where various compounds, including a gratuitous inducer and an anti-inducer of the mandelate enzymes, were added to uninduced or pre-induced cultures and to constitutive and blocked mutants. The results led to the conclusion that there were at least two types of repression. One was caused by phenylglyoxylate carboxy-lyase (or a compound synthesized co-ordinately with it), but not by the other mandelate enzymes or by (+)-mandelate, phenylglyoxylate, benzaldehyde or benzoate. A second type of repression was observed during rapid growth or after the addition of compounds such as succinate which are rapidly and completely metabolized.

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© Society for General Microbiology 1977
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1977-11-01
2024-04-26
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Regulation of Synthesis of Benzyl Alcohol Dehydrogenase in Acinetobacter calcoaceticusncib8250
Microbiology 103, 127 (1977); https://doi.org/10.1099/00221287-103-1-127
/content/journal/micro/10.1099/00221287-103-1-127
/content/journal/micro/10.1099/00221287-103-1-127
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