1887

Abstract

The ‘Phofil’ mutant of showed a disturbed adaptation with respect to a change from aerobic to photosynthetic conditions. The mutation was a pleiotropic one: firstly, the cells formed multinucleate filaments with occasional septa, and secondly, they integrated only a fraction of the synthetized pigments. The phenotypes appeared in photosynthetic conditions during a period of unbalanced growth (unadapted state) followed by balanced exponential growth where the phenotypes were corrected (adapted state). Ultraviolet light irradiation or exposure to low temperature, but not partial or total depigmentation, caused adapted cells to return to the unadapted state. Except for those associated with pigments, no appreciable defect in the polypeptide composition of the cell envelope was detected by SDS-polyacrylamide gel electrophoresis. However, the protein/phospholipid ratio of the envelope fraction was approximately 50 % higher, in the mutant than in the parent. These findings, and others described in the text, suggested the existence of defects in the cell wall and in the inner membrane.

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1977-10-01
2021-10-24
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References

  1. Barlett G. R. 1959; Phosphorus assay in column chromatography. Journal of Biological Chemistry 234:466–468
    [Google Scholar]
  2. Clayton R. K. 1963; Absorption spectra of photosynthetic bacteria and their chlorophylls. In Bacterial Photosynthesis pp. 498–500 Edited by Gest H., San Pietro A., Vernon L. P. Yellow Springs, Ohio: Antioch Press;
    [Google Scholar]
  3. Clayton R. K., Haselkorn R. 1972; Protein components of bacterial photosynthetic membranes. Journal of Molecular Biology 68:95–105
    [Google Scholar]
  4. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. Journal of Cellular and Comparative Physiology 49:25–68
    [Google Scholar]
  5. Drews G. 1974; Composition of a protochlorophyll-protopheophytin complex, excreted by mutant strains of Rhodopseudomonas capsulata, in comparison with the photosynthetic apparatus. Archives of Microbiology 100:397–407
    [Google Scholar]
  6. Irschik H., Oelze J. 1973; Membrane differentiation in phototrophically growing Rhodospirillum rubrum during transition from low to high light intensity. Biochimica et biophysica acta 330:80–89
    [Google Scholar]
  7. Jolchine G., Reiss-Husson F. 1974; Comparatives studies on two reaction center preparations from Rhodopseudomonas spheroides. FEBS Letters 40:5–8
    [Google Scholar]
  8. Kates M. 1972; Lipid extraction procedures. In Techniques in Lipodology pp. 347–353 Edited by Work T. S., Work E. Amsterdam: North Holland Publishing Company;
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  10. Latarjet R., Morenne P., Berger R. 1953; Un appareil simple pour le dosage des rayonne- ments ultra-violets 6mis par les lampes germicides. Annales de l’Institut Pasteur 85:174–178
    [Google Scholar]
  11. Leive L. 1974; The barrier function of the gramnegative envelope. Annals of the New York Academy of Sciences 235:109–129
    [Google Scholar]
  12. Lowry O. H., Roserrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Mcintyre T. M., Bell R. M. 1975; Mutant of Escherichia coli defective in membrane phospholipid synthesis. Effect of cessation of net phospholipid synthesis on cytoplasmic and outer membrane. Journal of Biological Chemistry 250:9053–9059
    [Google Scholar]
  14. Niederman R. A., Mallon D. E., Langan J. J. 1976; Membrane of Rhodopseudomonas spheroides. IV. Assembly of chromatophores in lowaeration cell suspension. Biochimica et biophysica acta 440:429–447
    [Google Scholar]
  15. Normark S., Wolf-Watz H. 1974; Cell division and permeability of unbalanced envelope mutants of Escherichia coli. Annales de Micro- biologie (Institut Pasteur) 125B:211–226
    [Google Scholar]
  16. Oelze J., Drews G. 1972; Membranes of photosynthetic bacteria. Biochimica et biophysica acta 265:209–239
    [Google Scholar]
  17. Oelze J., Pahlke W. 1976; The early formation of the photosynthetic apparatus in Rhodospirillum rubrum. Archives of Microbiology 108:281–285
    [Google Scholar]
  18. Peters G. A., Cellarius R. A. 1972; Photosynthetic membrane development in Rhodopseudomonas spheroides. II. Correlation of pigment incorporation with morphological aspects of thylakoid formation. Bioenergetics 3:345–359
    [Google Scholar]
  19. Pradel J., Clément-Métral J. 1976; A 4-vinyl- protochlorophyllide complex accumulated by ‘Phofil’ mutant of Rhodopseudomonas spheroides. An authentic intermediate in the development of the photosynthetic apparatus. Biochimica et biophysica acta 430:253–264
    [Google Scholar]
  20. Saunders V. A., Jones O. T. G. 1974; Adaptation in Rhodopseudomonas spheroides. FEBS Letters 44:169–172
    [Google Scholar]
  21. Schweitzer M., Schwarz H., Sonntag I., Henning U. 1976; Mutational change of membrane architecture. Mutants of Escherichia coli K12 missing major proteins of the outer cell envelope membrane. Biochimica et biophysica acta 448:474–491
    [Google Scholar]
  22. Slater M., Schaechter M. 1974; Control of cell division in bacteria. Bacteriological Reviews 38:199–221
    [Google Scholar]
  23. Spratt B. G., Pardee A. B. 1975; Penicillinbinding proteins and cell shape in Escherichia coli. Nature; London: 254516–517
    [Google Scholar]
  24. Starka J. 1971; Cell envelope proteins of dividing and non dividing cells of Escherichia coli. FEBS Letters 16:223–225
    [Google Scholar]
  25. Stone A. B. 1973; Regulation of cell division in a temperature-sensitive division mutant of Escherichia coli. Journal of Bacteriology 116:741–750
    [Google Scholar]
  26. Strominger J. L., Blumberg P. H., Suginaka H., Umbreit J. 1971; How penicillin kills bacteria: progress and problems. Proceedings of the Royal Society B179267–276
    [Google Scholar]
  27. Takemoto J., Lascelles J. 1973; Coupling between bacteriochlorophyll and membrane protein synthesis in Rhodopseudomonas spheroides. Proceedings of the National Academy of Sciences of the United States of America 70799–803
    [Google Scholar]
  28. Uspenskaya V. E., Lyskova G. V. 1972; Synthesis of pigments by the mutant of Rhodopseudomonas palustris under different growth conditions. Mikrobiologiya 6:1038–1044
    [Google Scholar]
  29. Walker J. R., Pardee A. B. 1967; Conditional mutations involving septum formation in Escherichia coli. Journal of Bacteriology 93:107–114
    [Google Scholar]
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