Summary: Crude extracts of were found to contain an endonuclease that degraded double-stranded linear DNA from bacteria and phages to fragments with a molecular weight of about 8 x 10. The nuclease did not have an absolute requirement for Mg. One discrete intermediate product had a molecular weight of 6.6 x 10. Extracts from two different mutants were tested: one completely lacked the endonuclease activity (strain db5575), and the other showed an absolute requirement for Mg (strain 4543). No biological role has yet been found for this endonuclease of


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