1887

Abstract

strains 11 and 5a1 which differ greatly in their ability to produce slime had similar ratios of nitrogenase activity when grown on agar under atmospheres of nitrogen, air or oxygen. With both strains, the rate of acetylene reduction was not significantly different when measured under air compared to nitrogen but was decreased under oxygen, which also restricted growth. Strain k11, the more slimy of the two strains produced more slime, as measured viscometrically, when the atmosphere above the agar contained a greater proportion of oxygen up to 50% O. Strain 5a1 gave no detectable response to increased oxygen concentration other than decreased growth and nitrogenase activity under high oxygen concentration. Despite the superficial correlation between enhanced slime production and resistance to the potentially damaging effects of oxygen on nitrogenase in air-grown nitrogen-fixing , slime probably contributes only marginally to that resistance.

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1977-08-01
2024-12-07
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References

  1. Dalton H., Postgate J. R. 1969; Effect of oxygen on growth of Azotobacter chroococcum in batch and continuous culture. Journal of General Microbiology 54:463–473
    [Google Scholar]
  2. Duguid J. P., Wilkinson J. F. 1961; Environmentally induced changes in bacterial morphology. Symposia of the Society for General Microbiology 11:69–99
    [Google Scholar]
  3. Hill S. 1971; Influence of oxygen concentration on the colony type of Derxia gummosa grown on nitrogen-free media. Journal of General Microbiology 67:77–83
    [Google Scholar]
  4. Hill S. 1975; Acetylene reduction by Klebsiella pneumoniae in air related to colony dimorphism on low fixed nitrogen. Journal of General Microbiology 91:207–209
    [Google Scholar]
  5. Hill S., Drozd J. W., Postgate J. R. 1972; Environmental effects on the growth of nitrogenfixing bacteria. Journal of Applied Chemistry and Biotechnology 22:541–558
    [Google Scholar]
  6. Jensen H. L., Petersen E. J., De P. K., Bhattacharya R. 1960; A new nitrogen-fixing bacterium: Derxia gummosa nov. gen. nov. spec. Archiv für Mikrobiologie 36:182–195
    [Google Scholar]
  7. Klucas R. 1972; Nitrogen fixation by Klebsiellagrown in the presence of oxygen. Canadian Journal of Microbiology 18:1845–1850
    [Google Scholar]
  8. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  9. Postgate J. R. 1971; Relevant aspects of the physiological chemistry of nitrogen fixation. Symposia of the Society for General Microbiology 21:287–307
    [Google Scholar]
  10. Tchan Y. T., Jensen H. L. 1963; Studies on nitrogen-fixing bacteria. VIII. Influence of N-content of the media on the N-fixation capacity and colony variation of Derxia gummosa Jensen et al. 1960. Proceedings of the Linnean Society of New South Wales 88:379–385
    [Google Scholar]
  11. Tubb R. S., Postgate J. R. 1973; Control of nitrogenase synthesis in Klebsiella pneumoniae. Journal of General Microbiology 79:103–117
    [Google Scholar]
  12. Werner D., Evans H. J., Seidler R. J. 1974; Facultatively anaerobic nitrogen-fixing bacteria from the marine environment. Canadian Journal of Microbiology 20:59–64
    [Google Scholar]
  13. Werner D., Wilcockson J., Zimmermann E. 1975; Adsorption and selection of rhizobia with ion-exchange papers. Archives of Microbiology 105:27–32
    [Google Scholar]
  14. Wilcockson J., Werner D. 1976; Nitrogenase activity by Klebsiella and Rhizobium on solid substrata exposed to air. Bericht der Deutschen botanischen Gesellschaft 89:587–607
    [Google Scholar]
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