1887

Abstract

The sensitivities of the binding step and the lytic step of haemolysis by pneumolysin to the action of various inhibitors and to variations in the assay conditions were studied. Binding was inhibited by HgCl and -ethylmaleimide. Lysis by previously fixed lysin was insensitive to HgCl and only slightly sensitive to -ethylmaleimide. Binding of pneumolysin was independent of ionic strength. Binding of pneumolysin and streptolysin O decreased above pH 8·0 and 8·4, respectively. These results suggest that binding requires a non-ionized unsubstituted sulphydryl group. Incubation of erythrocytes with NaF caused inhibition of pneumolysin, indicating that some metabolic function of the cell may be involved in lysis. The action of streptolysin O was not affected by NaF.

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/content/journal/micro/10.1099/00221287-101-2-237
1977-08-01
2021-05-11
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References

  1. Alouf J. E., Raynaud M. 1968a; Action de la Streptolysine O sur les membranes cellulaires.I.Fixation sur la membrane erythrocytaire.. Annales de l’Institut Pasteur 114:812–827
    [Google Scholar]
  2. Alouf J. E., Raynaud M. 1968b; Action de la Streptolysine O sur les membranes cellulaires.II.Cinétique de la lyze erythrocytaire.. Annales de l’Institut Pasteur 115:97–121
    [Google Scholar]
  3. Halbert S. P. 1970; Streptolysin O.. In Microbial Toxins 3 pp. 69–98 Edited by Montie T. C., Kadis S., Ajl S. A. New York: Academic Press;
    [Google Scholar]
  4. Johnson M. K. 1972; Properties of purified pneumococcal hemolysin.. Infection and Immunity 6:755–760
    [Google Scholar]
  5. Konbayashi Y., Hotta M., Koyama J. 1972; Kinetic study on Streptolysin O.. Journal of Biochemistry 71:227–237
    [Google Scholar]
  6. Oberley T. D., Duncan J. L. 1971; Characteristics of Streptolysin O action.. Infection and Immunity 4:683–687
    [Google Scholar]
  7. Ramm L. E., Winkler H. H. 1973; Rickettsial hemolysis: effect of metabolic inhibitors upon hemolysis and adsorption.. Infection andlmmunity 7:550–555
    [Google Scholar]
  8. Smythe C. V., Harris T. N. 1940; Some properties of a hemolysin produced by group A β-hemolytic streptococci.. Journal of Immunology 38:283–300
    [Google Scholar]
  9. Stock A. H., Uriel J. 1961; Electrophoretic mobility and detection of hemolytic activity of streptolysins O and S in agar gel.. Nature, London 192:435–436
    [Google Scholar]
  10. Todd E. W. 1934; A comparative serological study of streptolysins derived from human and from animal infections, with notes on pneumococcal haemolysin, tetanolysin and staphylococcus toxin.. Journal of Pathology and Bacteriology 39:299–321
    [Google Scholar]
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