1887

Abstract

Summary: Five mutants of strain 2 (ColE1), initially detected because they released little or no colicin when tested on solid medium, proved to be sensitive to ultraviolet light (u.v.). Further testing indicated that one of the mutants was deficient in genetic recombination and was probably a -type mutant, while three of the others were deficient in DNA polymerase activity and appeared to be typical mutants. The fifth mutant was less sensitive than the others to methyl methanesulphonate, showed reduced proficiency in genetic recombination, and was of approximately normal u.v. mutability. This mutant may be a counterpart of the class known as in . All five mutants degraded significantly more of their DNA following exposure to u.v. than did the wild-type strain. The -type mutant and the possible mutant also degraded significantly more of their DNA spontaneously than did the wild-type. Treatment with visible light and acridine orange (photodynamic treatment) caused no significant degradation of DNA in the wild-type strain, a highly significant increase in the extent of DNA degradation in a mutant, and a decrease in the extent of degradation in the -type mutant.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-101-1-143
1977-07-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/101/1/mic-101-1-143.html?itemId=/content/journal/micro/10.1099/00221287-101-1-143&mimeType=html&fmt=ahah

References

  1. Boyle J. M., Paterson M. C., Setlow R. B. 1970; Excision repair properties of an Escherichia coli mutant deficient in DNA polymerase. Nature; London: 266:708–710
    [Google Scholar]
  2. Dellweg H., Opree W. 1966; Die photodynamische Wirkung von Thiopyronin auf Nucleinsäuren. Biophysik 3:241–248
    [Google Scholar]
  3. Ferron W. L., Eisenstark A., Mackay D. 1972; Distinction between far- and near-ultraviolet killing of recombinationless (recA) Salmonella typhimurium . Biochimica et biophysica acta 227:651–658
    [Google Scholar]
  4. Guerola N., Ingraham I. L., Cerdá-Olmedo E. 1971; Induction of closely linked multiple mutations by nitrosoguanidine. Nature New Biology 230:122–125
    [Google Scholar]
  5. Howard-Flanders P., Boyce R. B. 1966; DNA repair and genetic recombination: studies on mutants of E. coli defective in these processes. Radiation Research Supp. 6:156–184
    [Google Scholar]
  6. Imray F. P., Macphee D. G. 1973; The role of DNA polymerase I and the rec system in survival of bacteria and bacteriophages damaged by the photodynamic action of acridine orange. Molecular and General Genetics 123:289–298
    [Google Scholar]
  7. De Lucia P., Cairns J. 1969; Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature; London: 224:1164–1166
    [Google Scholar]
  8. Macphee D. G. 1970; Recombination-deficient mutants of colicinogenic strains of Salmonella typhimurium detected by their failure to produce colicin. Journal of Bacteriology 104:345–350
    [Google Scholar]
  9. Macphee D. G. 1973; Effects of an R factor and caffeine on ultraviolet mutability in Salmonella typhimurium . Mutation Research 18:367–370
    [Google Scholar]
  10. Macphee D. G., Beazer M. R. 1973; Mutants of Salmonella typhimurium deficient in DNA polymerase I: detection by their failure to produce colicin El. Molecular and General Genetics 127:229–240
    [Google Scholar]
  11. Macphee D. G., Beazer M. R. 1975; Mutants of Salmonella typhimurium deficient in DNA polymerase I: further characterization and genetic analysis. Australian Journal of Biological Sciences 28:559–565
    [Google Scholar]
  12. Ogawa H. 1970; Genetic locations of uvrD and pol genes of E. coli . Molecular and General Genetics 108:378–381
    [Google Scholar]
  13. Ogawa H., Shimida K., Tomizawa A. 1968; Studies on radiation-sensitive mutants of E. coli. I. Mutants defective in the repair synthesis. Molecular and General Genetics 101:227–244
    [Google Scholar]
  14. Smith K. C. 1962; Dose dependent decrease in extractability of DNA from bacteria following irradiation with ultraviolet light or with visible light plus dye. Biochemical and Biophysical Research Communications 8:157–163
    [Google Scholar]
  15. Spikes J.D, Livingstone R. 1969; The molecular biology of photodynamic action: sensitized photautoxidations in biological systems. Advances in Radiation Biology 3:29–121
    [Google Scholar]
  16. Willetts N. S., Clark A. J. 1969; Characteristics of some multiply recombination-deficient strains of E. coli . Journal of Bacteriology 100:231–239
    [Google Scholar]
  17. Wing J. P., Levine M., Smith H. O. 1968; Recombination-deficient mutant of Salmonella typhimurium . Journal of Bacteriology 95:1828–1834
    [Google Scholar]
  18. Youngs D. A., Bernstein I. A. 1973; Involvement of the recB-recC nuclease (exonuclease V) in the process of X-ray induced deoxyribonucleic acid degradation in radiosensitive strains of Escherichia coli KI2. Journal of Bacteriology 113:901–906
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-101-1-143
Loading
/content/journal/micro/10.1099/00221287-101-1-143
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error