1887

Abstract

SUMMARY: A partial order for genes which specify -cleavage pathway enzymes has been derived from properties of a polarity mutant strain (PsU5) of 10015 and a partial revertant, PsU5/R21. The polar mutation is within the 2-hydroxymuconic semialdehyde dehydrogenase gene and results in loss of detectable 2-hydroxymuconic semialdehyde hydrolase and 90 to 95 % reduction in the activities of 4-oxalocrotonate tautomerase, 4-oxalocrotonate decarboxylase, 2-oxopent-4-enoate hydratase and 4-hydroxy-2-oxovalerate aldolase. The partial revertant PsU5/R21 regained wild-type levels of all enzymes except the aldehyde dehydrogenase.

It is probable that all genes determining the dehydrogenase and subsequent enzymes are transcribed as a polycistronic message. This is the first report of mapping genes determining the enzymes of the -cleavage pathway.

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/content/journal/micro/10.1099/00221287-100-1-65
1977-05-01
2024-12-07
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References

  1. Bayly R. C., Wigmore G. J. 1973; Metabolism of phenol and cresols by mutants of Pseudomonas putida. Journal of Bacteriology 113:1112–1120
    [Google Scholar]
  2. Bayly R. C., Wigmore G. J., Mckenzie D. I. 1977; Regulation of the enzymes of the meta-cleavage pathway of Pseudomonas putida: the regulon is composed of two operons. Journal of General Microbiology 100:71–79
    [Google Scholar]
  3. Feist C. F., Hegeman G. D. 1969; Phenol and benzoate metabolism by Pseudomonas putida: regulation of tangential pathways. Journal of Bacteriology 100:869–877
    [Google Scholar]
  4. Files J. G., Weber K., Miller J. H. 1974; Translational reinitiation: reinitiation of lac repressor fragments at three internal sites early in the lac i gene of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 71667–670
    [Google Scholar]
  5. Ganem D., Miller J. H., Files J. C., Platt T., Weber K. 1973; Reinitiation of a lac repressor fragment at a codon other than AUG. Proceedings of the National Academy of Sciences of the United States of America 703165–3169
    [Google Scholar]
  6. Holloway B. W. 1975; Genetic organization of Pseudomonas. In Genetics and Biochemistry of Pseudo-monas pp. 133–161 Clarke P. H., Richmond M. H. Edited by London: John Wiley;
    [Google Scholar]
  7. Morse D. E., Guertin M. 1971; Regulation of mRNA utilization and degradation by amino-acid starvation. Nature New Biology 232:165–169
    [Google Scholar]
  8. Ornston L. N. 1966; The conversion of catechol and protocatechuate to β-ketoadipate by Pseudomonas putida. IV. Regulation. Journal of Biological Chemistry 341:3800–3810
    [Google Scholar]
  9. Platt T., Weber K., Ganem D., Miller J. H. 1972; Translation restarts: AUG reinitiation of a lac repressor fragment. Proceedings of the National Academy of Sciences of the United States of America 69897–901
    [Google Scholar]
  10. Rechler M. M., Martin R. G. 1970; The intercistronic divide: translation of an intercistronic region in the histidine operon of Salmonella typhimurium. Nature; London: 226908–911
    [Google Scholar]
  11. Rechler M. M., Bruni C. B., Martin R. G., Terry W. 1972; An intercistronic region in the histidine operon of Salmonella typhimurium. Journal of Molecular Biology 69:427–452
    [Google Scholar]
  12. Reznikoff W. S., Michels C. A., Cooper T. G., Silverstone A. E., Magasanik B. 1974; Inhibition of lacZ gene translation initiation in trp-lac fusion strains. Journal of Bacteriology 117:1231–1239
    [Google Scholar]
  13. Stanisich V. A., Richmond M. H. 1975; Gene transfer in the genus Pseudomonas. In Genetics and Bio-chemistry of Pseudomonas pp. 163–190 Clarke P. H., Richmond M. H. Edited by London: John Wiley;
    [Google Scholar]
  14. Voll M. J. 1967; Translation and polarity in the histidine operon. III. The isolation of prototrophic polar mutants. Journal of Molecular Biology 30:109–124
    [Google Scholar]
  15. Wheelis M. L., Ornston L. N. 1972; Genetic control of enzyme induction in the β-ketoadipate pathway of Pseudomonas putida: deletion mapping of cat mutations. Journal of Bacteriology 109:790–795
    [Google Scholar]
  16. Wigmore G. J. 1975 Metabolism of phenol and cresols by mutant strains of Pseudomonas putida Ph.D. thesis Monash University, Australia;
    [Google Scholar]
  17. Wigmore G. J., Bayly R. C. 1974; A mutant of Pseudomonas putida with altered regulation of the enzymes for degradation of phenol and cresols. Biochemical and Biophysical Research Communications 60:48–55
    [Google Scholar]
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