1887

Abstract

The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system is an important adaptive immune system for bacteria to resist foreign DNA infection, which has been widely used in genotyping and gene editing. To provide a theoretical basis for the application of the CRISPR-Cas system in , the occurrence and diversity of CRISPR-Cas systems were analysed in 150 strains. Specifically, 47 % (71/150) of genomes possessed the CRISPR-Cas system, and type I-C CRISPR-Cas system was the most widely distributed among those strains. The spacer sequences present in can be used as a genotyping marker. Additionally, the phage assembly-related proteins were important targets of the type I-C CRISPR-Cas system in , and the protospacer adjacent motif sequences were further characterized in type I-C system as 5′-TTC-3′. All these results might provide a molecular basis for the development of endogenous genome editing tools in .

Funding
This study was supported by the:
  • Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province (Award Not Applicable)
    • Principle Award Recipient: JianxinZhao
  • National Natural Science Foundation of China (Award 31820103010)
    • Principle Award Recipient: WeiChen
  • 111 Project (Award BP0719028)
    • Principle Award Recipient: BoYang
  • National Natural Science Foundation of China (Award Nos. 32021005)
    • Principle Award Recipient: BoYang
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2022-04-22
2022-05-22
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