RT Journal Article SR Electronic(1) A1 Han, Xiao A1 Zhou, Xingya A1 Pei, Zhangming A1 Stanton, Catherine A1 Ross, R. Paul A1 Zhao, Jianxin A1 Zhang, Hao A1 Yang, Bo A1 Chen, WeiYR 2022 T1 Characterization of CRISPR-Cas systems in Bifidobacterium breve JF Microbial Genomics, VO 8 IS 4 OP SP 000812 DO https://doi.org/10.1099/mgen.0.000812 PB Microbiology Society, SN 2057-5858, AB The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system is an important adaptive immune system for bacteria to resist foreign DNA infection, which has been widely used in genotyping and gene editing. To provide a theoretical basis for the application of the CRISPR-Cas system in Bifidobacterium breve , the occurrence and diversity of CRISPR-Cas systems were analysed in 150 B. breve strains. Specifically, 47 % (71/150) of B. breve genomes possessed the CRISPR-Cas system, and type I-C CRISPR-Cas system was the most widely distributed among those strains. The spacer sequences present in B. breve can be used as a genotyping marker. Additionally, the phage assembly-related proteins were important targets of the type I-C CRISPR-Cas system in B. breve , and the protospacer adjacent motif sequences were further characterized in B. breve type I-C system as 5′-TTC-3′. All these results might provide a molecular basis for the development of endogenous genome editing tools in B. breve ., UL https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000812