@article{mbs:/content/journal/mgen/10.1099/mgen.0.000812, author = "Han, Xiao and Zhou, Xingya and Pei, Zhangming and Stanton, Catherine and Ross, R. Paul and Zhao, Jianxin and Zhang, Hao and Yang, Bo and Chen, Wei", title = "Characterization of CRISPR-Cas systems in Bifidobacterium breve", journal= "Microbial Genomics", year = "2022", volume = "8", number = "4", pages = "", doi = "https://doi.org/10.1099/mgen.0.000812", url = "https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000812", publisher = "Microbiology Society", issn = "2057-5858", type = "Journal Article", keywords = "genotyping", keywords = "prophage", keywords = "CRISPR-Cas system", keywords = "Bifidobacterium breve", eid = "000812", abstract = "The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system is an important adaptive immune system for bacteria to resist foreign DNA infection, which has been widely used in genotyping and gene editing. To provide a theoretical basis for the application of the CRISPR-Cas system in Bifidobacterium breve , the occurrence and diversity of CRISPR-Cas systems were analysed in 150 B. breve strains. Specifically, 47 % (71/150) of B. breve genomes possessed the CRISPR-Cas system, and type I-C CRISPR-Cas system was the most widely distributed among those strains. The spacer sequences present in B. breve can be used as a genotyping marker. Additionally, the phage assembly-related proteins were important targets of the type I-C CRISPR-Cas system in B. breve , and the protospacer adjacent motif sequences were further characterized in B. breve type I-C system as 5′-TTC-3′. All these results might provide a molecular basis for the development of endogenous genome editing tools in B. breve .", }