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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 57, Issue 6

TxA and SLP-36kDa enhance the immune response toward a co-administered antigen No Access

Abstract

This study evaluated the adjuvant activity of two peptides derived from : a fragment of the receptor-binding domain of toxin A (TxA) and a fragment of the 36 kDa surface-layer protein (SLP-36kDa) from strain C253. Their ability to affect the magnitude, distribution and polarization of the immune response against fibronectin-binding protein A (FnbpA), a protective vaccine antigen against , was evaluated using two different routes of immunization: intranasal and subcutaneous. It was shown that (i) the route of immunization affected the magnitude of the immune response; (ii) both peptides enhanced the production of circulating anti-FnbpA IgG and IgA; (iii) following mucosal immunization TxA was more effective than SLP-36kDa at inducing antibody in the gastrointestinal tract; (iv) the adjuvant influenced the Th1/Th2 balance; and (v) TxA was more effective than SLP-36kDa in inducing a cell-mediated response. These studies provide insight into the ability of different -derived peptides to differentially affect and polarize the activity of the immune system and on their potential use as adjuvants in newly developed vaccines.

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  • Accepted:
  • Published Online:
Keyword(s): CT, cholera toxin , DC, dendritic cell , FnbpA, fibronectin-binding protein A , i.m., intramuscularly , i.n., intranasally , IFN-γ, gamma interferon , IL-2, interleukin-2 , LT, heat-labile enterotoxin , s.c., subcutaneously , SLP, surface-layer protein , SLP-36kDa, fragment of the 36 kDa SLP and TcdA, toxin A
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/content/journal/jmm/10.1099/jmm.0.47736-0
2008-06-01
2025-04-24
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/content/journal/jmm/10.1099/jmm.0.47736-0
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Clostridium difficile TxAC314 and SLP-36kDa enhance the immune response toward a co-administered antigen
J. Med. Microbiol. 57, 725 (2008); https://doi.org/10.1099/jmm.0.47736-0
/content/journal/jmm/10.1099/jmm.0.47736-0
/content/journal/jmm/10.1099/jmm.0.47736-0
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