1887

Abstract

A molecular epidemiological survey was conducted to investigate the presence of pathogenic () species in the forest areas of Zhejiang province, south-east China. A total of 182 ticks of 6 species and 200 rodents of 8 species were collected and individually examined for the presence of DNA by nested PCR targeting the 5S–23S rRNA intergenic spacer. Forty-one ticks of four species, , , and , were infected with , with an overall infection rate of 23 %. Sixteen rodents of four species, , , and , were positive for , with an overall prevalence of 8 %. I RFLP analysis and sequence analysis of the positive PCR products showed that spirochaetes in specimens consisted of , and -related group. Forty (98 %) of the -positive ticks were infected with and one (2 %) was infected with . Twelve (75 %) of the positive rodents were infected with and four (25 %) were infected with the spirochaete belonging to -related group.

Keyword(s): LD, Lyme disease
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47663-0
2008-08-01
2019-11-12
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/8/980.html?itemId=/content/journal/jmm/10.1099/jmm.0.47663-0&mimeType=html&fmt=ahah

References

  1. Ai, C. X., Wen, Y. X., Zhang, Y. G., Wang, S. S., Qiu, G. C., Shi, Z. X., Cheng, Z. Q., Li, D. Y. & Liu, X. D. ( 1987; ). Epidemiological study on Lyme disease in Hailin of Heilongjiang. Chin Public Health 6, 82–85.
    [Google Scholar]
  2. Anderson, J. F. ( 1991; ). Epizootiology of Lyme borreliosis. Scand J Infect Dis Suppl 77, 23–34.
    [Google Scholar]
  3. Balmelli, T. & Piffaretti, J. C. ( 1995; ). Association between different clinical manifestations of Lyme disease and different species of Borrelia burgdorferi sensu lato. Res Microbiol 146, 329–340.[CrossRef]
    [Google Scholar]
  4. Baranton, G., Postic, D., Saint Girons, I., Boerlin, P., Piffaretti, J. C., Assous, M. & Grimont, P. A. ( 1992; ). Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int J Syst Bacteriol 42, 378–383.[CrossRef]
    [Google Scholar]
  5. Burgdorfer, W., Anderson, J. F., Gern, L., Lane, R. S., Piesman, J. & Spielman, A. ( 1991; ). Relationship of Borrelia burgdorferi to its arthropod vectors. Scand J Infect Dis Suppl 77, 35–40.
    [Google Scholar]
  6. Cao, W. C., Gao, Y. M., Zhang, P. H., Zhang, X. T., Dai, Q. H., Dumler, J. S., Fang, L. Q. & Yang, H. ( 2000; ). Identification of Ehrlichia chaffeensis by nested PCR in ticks from southern China. J Clin Microbiol 38, 2778–2780.
    [Google Scholar]
  7. Cheng, Y. J., Zhang, Z. D. & Ye, M. J. ( 1992; ). One Lyme disease case report. Zhejiang Prevent Med Dis Surveil 4, 2
    [Google Scholar]
  8. Chu, C. Y., He, J., Wang, J. B., Hasen, G. W., Zhang, P. H., Wu, X. M., Zhao, Q. M., Jiang, B. G., Gao, Y. & Cao, W. C. ( 2006; ). Investigation on Borrelia burgdorferi sensu lato in ticks and rodents collected in Da Xing-An Mountains Forest areas of China. Chin J Epidemiol 27, 681–684.
    [Google Scholar]
  9. Collares-Pereira, M., Couceiro, S., Franca, I., Kurtenbach, K., Schäfer, S. M., Vitorino, L., Gonçalves, L., Baptista, S., Vieira, M. L. & Cunha, C. ( 2004; ). First isolation of Borrelia lusitaniae from a human patient. J Clin Microbiol 42, 1316–1318.[CrossRef]
    [Google Scholar]
  10. Foldvari, G., Farkas, R. & Lakos, A. ( 2005; ). Borrelia spielmanii erythema migrans, Hungary. Emerg Infect Dis 11, 1794–1795.[CrossRef]
    [Google Scholar]
  11. Hanincová, K., Taragelova, V., Koci, J., Schafer, S. M., Hails, R., Ullmann, A. J., Piesman, J., Labuda, M. & Kurtenbach, K. ( 2003; ). Association of Borrelia garinii and B. valaisiana with songbirds in Slovakia. Appl Environ Microbiol 69, 2825–2830.[CrossRef]
    [Google Scholar]
  12. Hengge, U. R., Tannapfel, A., Tyring, S. K., Erbel, R., Arendt, G. & Ruzicka, T. ( 2003; ). Lyme borreliosis. Lancet Infect Dis 3, 489–500.[CrossRef]
    [Google Scholar]
  13. Humair, P. F., Postic, D., Wallich, R. & Gern, L. ( 1998; ). An avian reservoir (Turdus merula) of the Lyme borreliosis spirochetes. Zentralbl Bakteriol 287, 521–538.
    [Google Scholar]
  14. Kee, S. H., Yoon, J. H., Oh, H. B., Park, Y. H., Kim, Y. W., Cho, M. K., Park, K. S. & Chang, W. H. ( 1996; ). Genetic analysis of Borrelia burgdorferi sensu lato in Korea using genomic hybridization and 16S rRNA gene sequence determination. Microbiol Immunol 40, 599–605.[CrossRef]
    [Google Scholar]
  15. Kurtenbach, K., Peacey, M., Rijpkema, S. G., Hoodless, A. N., Nuttall, P. A. & Randolph, S. E. ( 1998a; ). Differential transmission of the genospecies of Borrelia burgdorferi sensu lato by game birds and small rodents in England. Appl Environ Microbiol 64, 1169–1174.
    [Google Scholar]
  16. Kurtenbach, K., Sewell, H. S., Ogden, N. H., Randolph, S. E. & Nuttall, P. A. ( 1998b; ). Serum complement sensitivity as a key factor in Lyme disease ecology. Infect Immun 66, 1248–1251.
    [Google Scholar]
  17. Lu, G. & Moriyama, E. N. ( 2004; ). Vector NTI, a balanced all-in-one sequence analysis suite. Brief Bioinform 5, 378–388.[CrossRef]
    [Google Scholar]
  18. Masuzawa, T., Fukui, T., Miyake, M., Oh, H. B., Cho, M. K., Chang, W. H., Imai, Y. & Yanagihara, Y. ( 1999; ). Determination of members of a Borrelia afzelii-related group isolated from Ixodes nipponensis in Korea as Borrelia valaisiana. Int J Syst Bacteriol 49, 1409–1415.[CrossRef]
    [Google Scholar]
  19. Masuzawa, T., Takada, N., Kudeken, M., Fukui, T., Yano, Y., Ishiguro, F., Kawamura, Y., Imai, Y. & Ezaki, T. ( 2001; ). Borrelia sinica sp. nov., a Lyme disease-related Borrelia species isolated in China. Int J Syst Evol Microbiol 51, 1817–1824.[CrossRef]
    [Google Scholar]
  20. Masuzawa, T., Hashimoto, N., Kudeken, M., Kadosaka, T., Nakamura, M., Kawabata, H., Koizumi, N. & Imai, Y. ( 2004; ). New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan. J Med Microbiol 53, 421–426.[CrossRef]
    [Google Scholar]
  21. Picken, R. N., Cheng, Y., Strle, F. & Picken, M. M. ( 1996; ). Patient isolates of Borrelia burgdorferi sensu lato with genotypic and phenotypic similarities of strain 25015. J Infect Dis 174, 1112–1115.[CrossRef]
    [Google Scholar]
  22. Postic, D., Assous, M. V., Grimont, P. A. & Baranton, G. ( 1994; ). Diversity of Borrelia burgdorferi sensu lato evidenced by restriction fragment length polymorphism of rrf (5S)-rrl (23S) intergenic spacer amplicons. Int J Syst Bacteriol 44, 743–752.[CrossRef]
    [Google Scholar]
  23. Richter, D., Postic, D., Sertour, N., Livey, I., Matuschka, F. R. & Baranton, G. ( 2006; ). Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov. Int J Syst Evol Microbiol 56, 873–881.[CrossRef]
    [Google Scholar]
  24. Rijpkema, S. G., Tazelaar, D. J., Molkenboer, M. J., Noordhoek, G. T., Plantinga, G., Schouls, L. M. & Schellekens, J. F. ( 1997; ). Detection of Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii and group VS116 by PCR in skin biopsies of patients with erythema migrans and acrodermatitis chronica atrophicans. Clin Microbiol Infect 3, 109–116.[CrossRef]
    [Google Scholar]
  25. Schwartz, J. J., Gazumyan, A. & Schwartz, I. ( 1992; ). rRNA gene organization in the Lyme disease spirochete, Borrelia burgdorferi. J Bacteriol 174, 3757–3765.
    [Google Scholar]
  26. Steere, A. C., Grodzicki, R. L., Kornblatt, A. N., Craft, J. E., Barbour, A. G., Burgdorfer, W., Schmid, G. P., Johnson, E. & Malawista, S. E. ( 1983; ). The spirochetal etiology of Lyme disease. N Engl J Med 308, 733–740.[CrossRef]
    [Google Scholar]
  27. Sun, Y., Xu, R. & Cao, W. ( 2003; ). Ixodes sinensis: competence as a vector to transmit the Lyme disease spirochete Borrelia garinii. Vector Borne Zoonotic Dis 3, 39–44.[CrossRef]
    [Google Scholar]
  28. Teng, K. F. & Jiang, Z. J. ( 1991; ). Economic Insect Fauna of China, 2nd edn. Beijing: Science Press.
  29. van Dam, A. P., Kuiper, H., Vos, K., Widjojokusumo, A., de Jongh, B. M., Spanjaard, L., Ramselaar, A. C., Kramer, M. D. & Dankert, J. ( 1993; ). Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin Infect Dis 17, 708–717.[CrossRef]
    [Google Scholar]
  30. Wan, K., Zhang, Z. & Dou, G. ( 1998; ). Investigation on primary vectors of Borrelia burgdorferi in China. Chin J Epidemiol 19, 263–266.
    [Google Scholar]
  31. Wang, C. X. ( 1997; ). Ecology and Control of Rodents, 1st edn. Beijing,: Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine.
  32. Wang, G., van Dam, A. P., Schwartz, I. & Dankert, J. ( 1999; ). Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. Clin Microbiol Rev 12, 633–653.
    [Google Scholar]
  33. Zhang, Z. F., Wan, K. L. & Zhang, J. S. ( 1997; ). Studies on epidemiology and etiology of Lyme disease in China. Chin J Epidemiol 18, 8–11.
    [Google Scholar]
  34. Zhang, Z. D., Cheng, Y. J., Mei, Z. H., Wang, D. B., Lin, R. W., Wu, W. Y., Lv, L. Z., Wang, C. & Shi, G. P. ( 2001; ). Seroepidemiological Survey on human Lyme Disease in the southern part of Zhejiang province. Chin Public Health 17, 1036–1039.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47663-0
Loading
/content/journal/jmm/10.1099/jmm.0.47663-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error