1887

Abstract

is a major pathogen associated with bovine mastitis, one of the most important infectious diseases occurring in dairy cattle herds worldwide. In the present study, isolates recovered from cows with mastitis in dairy herds located in the south-east of Brazil were genotyped by PFGE and multilocus sequence typing (MLST). PFGE identified 60 pulsotypes (PTs), which were found to be distributed among six clonal complexes (CCs) by MLST. All PTs with similarity percentages greater than 65 % belonged to the same CC. Most of the PTs belonged to CC126 (=28) and CC97 (=19), which were represented by 91 % of the isolates. These CCs have also been recovered from cows with mastitis in countries located in different continents, but they have rarely been isolated from human specimens. Few isolates were represented by PTs belonging to CCs that are frequently isolated from human specimens (CC1, CC5 and CC30). These data reinforce the hypothesis that a limited number of CCs are responsible for most bovine mastitis cases internationally. Specific features of the specialized clones should be studied for use as future targets of mastitis control measures.

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2007-11-01
2019-10-18
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References

  1. Aguiar-Alves, F., Medeiros, F., Fernandes, O., Pereira, R. M. G., Perdreau-Remington, F. & Riley, L. W. ( 2006; ). New Staphylococcus aureus genotyping method based on exotoxin (set) genes. J Clin Microbiol 44, 2728–2732.[CrossRef]
    [Google Scholar]
  2. Aires-de-Sousa, M., Parente, C. E. S. R., Vieira-da-Motta, O., Bonna, I. C. F., Silva, D. A. & Lencastre, H. ( 2007; ). Characterization of Staphylococcus aureus isolates from buffalo, bovine, ovine, and caprine milk samples collected in Rio de Janeiro state, Brazil. Appl Environ Microbiol 73, 3845–3849.[CrossRef]
    [Google Scholar]
  3. Brito, M. A. V. P., Brito, J. R. F., Ribeiro, M. T. & Veiga, V. M. O. ( 1999; ). Padrão de infecção de intramamária em rebanhos leiteiros: exame de todos os quartos mamários das vacas em lactação. Arq Bras Med Vet Zootec 51, 129–135. (in Brazilian)[CrossRef]
    [Google Scholar]
  4. Enright, M. C. & Spratt, B. G. ( 1999; ). Multilocus sequence typing. Trends Microbiol 7, 482–487.[CrossRef]
    [Google Scholar]
  5. Enright, M. C., Day, N. P. J., Davies, C. E., Peacock, S. J. & Spratt, B. G. ( 2000; ). Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38, 1008–1015.
    [Google Scholar]
  6. Enright, M. C., Robinson, D. A., Randle, G., Feil, E. J., Grundmann, H. & Spratt, B. G. ( 2002; ). The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc Natl Acad Sci U S A 99, 7687–7692.[CrossRef]
    [Google Scholar]
  7. Feil, E. J., Copper, J. E., Grundmann, H., Robinson, D. A., Enright, M. C., Berendt, T., Peacock, S. J., Smith, J. M., Murphy, M. & other authors ( 2003; ). How clonal is Staphylococcus aureus?. J Bacteriol 185, 3307–3316.[CrossRef]
    [Google Scholar]
  8. Feil, E. J., Li, B. C., Aanensen, D. M., Hanage, W. P. & Spratt, B. G. ( 2004; ). eburst: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186, 1518–1530.[CrossRef]
    [Google Scholar]
  9. Fitzgerald, J. R., Meaney, W. J., Hartigan, P. J., Smyth, C. J. & Kapur, V. ( 1997; ). Fine-structure molecular epidemiological analysis of Staphylococcus aureus recovered from cows. Epidemiol Infect 119, 261–269.[CrossRef]
    [Google Scholar]
  10. Gomes, A. R., Westh, H. & de Lencastre, H. ( 2006; ). Origins and evolution of methicillin-resistant Staphylococcus aureus clonal lineages. Antimicrob Agents Chemother 50, 3237–3244.[CrossRef]
    [Google Scholar]
  11. Joo, Y. S., Fox, L. K., Davis, W. C., Bohach, G. A. & Park, Y. H. ( 2001; ). Staphylococcus aureus associated with mammary glands of cows: genotyping to distinguish different strains among herds. Vet Microbiol 80, 131–138.[CrossRef]
    [Google Scholar]
  12. Jorgensen, H. J., Mork, T., Caugant, D. A., Kearns, A. & Rorvik, L. M. ( 2005; ). Genetic variation among Staphylococcus aureus strains from Norwegian bulk milk. Appl Environ Microbiol 71, 8352–8361.[CrossRef]
    [Google Scholar]
  13. Kapur, V., Sischo, W. M., Greer, R. S., Whittman, T. S. & Musser, J. M. ( 1995; ). Molecular population genetic analysis of Staphylococcus aureus recovered from cows. J Clin Microbiol 33, 376–380.
    [Google Scholar]
  14. Kwon, N. H., Park, K. T., Moon, J. S., Jung, W. K., Kim, S. H., Kim, J. M., Hong, S. K., Koo, H. C., Joo, Y. S. & Park, Y. O. ( 2005; ). Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea. J Antimicrob Chemother 56, 624–632.[CrossRef]
    [Google Scholar]
  15. Larsen, H. D., Sloth, K. H., Elsberg, C., Enevoldsen, C., Pedersen, L. H., Eriksen, N. H., Aarestrup, F. M. & Jensen, N. E. ( 2000; ). The dynamics of Staphylococcus aureus intramammary infection in nine Danish dairy herds. Vet Microbiol 71, 89–101.[CrossRef]
    [Google Scholar]
  16. Layer, F., Ghebremedhin, B., Konig, W. & Konig, B. ( 2006; ). Heterogeneity of methicillin-susceptible Staphylococcus aureus strains at a German University Hospital implicates the circulating-strain pool as a potential source of emerging methicillin-resistant S. aureus clones. J Clin Microbiol 44, 2179–2185.[CrossRef]
    [Google Scholar]
  17. Lee, J. H. ( 2003; ). Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol 69, 6489–6494.[CrossRef]
    [Google Scholar]
  18. Lovseth, A., Loncarevic, S. & Berdal, K. G. ( 2004; ). Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates. J Clin Microbiol 42, 3869–3872.[CrossRef]
    [Google Scholar]
  19. Matthews, K. R., Kumar, S. J., O'Conner, S. A., Harmon, R. J., Pankey, J. W., Fox, L. K. & Oliver, S. P. ( 1994; ). Genomic fingerprints of Staphylococcus aureus of bovine origin by polymerase chain reaction-based DNA fingerprinting. Epidemiol Infect 112, 177–186.[CrossRef]
    [Google Scholar]
  20. Mork, T., Tollersrud, T., Kvitle, B., Jorgensen, H. J. & Waage, S. ( 2005; ). Comparison of Staphylococcus aureus genotypes recovered from cases of bovine, ovine and caprine mastitis. J Clin Microbiol 43, 3979–3984.[CrossRef]
    [Google Scholar]
  21. National Mastitis Council ( 1999; ). Laboratory Handbook on Bovine Mastitis. Madison, WI: National Mastitis Council.
  22. Rabello, R. F., Souza, C. R., Duarte, R. S., Lopes, R. M., Teixeira, L. M. & Castro, A. C. ( 2005; ). Characterization of Staphylococcus aureus isolates recovered from bovine mastitis in Rio de Janeiro, Brazil. J Dairy Sci 88, 3211–3219.[CrossRef]
    [Google Scholar]
  23. Ribeiro, A., Dias, C., Silva-Carvalho, M. C., Berquó, L., Ferreira, F. A., Santos, R. N. S., Ferreira-Carvalho, B. T. & Figueiredo, A. M. ( 2005; ). First report of infection with community-acquired methicillin-resistant Staphylococcus aureus in South America. J Clin Microbiol 43, 1985–1988.[CrossRef]
    [Google Scholar]
  24. Robinson, D. A. & Enright, M. C. ( 2004; ). Multilocus sequence typing and the evolution of methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 10, 92–97.[CrossRef]
    [Google Scholar]
  25. Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E., Fox, L. K., Schukken, Y. H., Kruze, J. V., Bradley, A. J., Zadoks, R. N. & Dowson, C. G. ( 2005a; ). Multilocus sequence typing of intercontinental bovine Staphylococcus aureus isolates. J Clin Microbiol 43, 4737–4743.[CrossRef]
    [Google Scholar]
  26. Smith, E. M., Green, L. E., Medley, G. F., Bird, H. E. & Dowson, C. G. ( 2005b; ). Multilocus sequence typing of Staphylococcus aureus isolated from high-somatic-cell-count cows and the environment of an organic dairy farm in the United Kingdom. J Clin Microbiol 43, 4731–4736.[CrossRef]
    [Google Scholar]
  27. Tenover, F. C., Arbeit, R. D., Goering, R. V., Mickelsen, P. A., Murray, B. E., Persing, D. H. & Swaminathan, B. ( 1995; ). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33, 2233–2239.
    [Google Scholar]
  28. Vivoni, A. M., Diep, B. A., Magalhães, A. C. G., Santos, K. R. N., Riley, L. W., Sensabaugh, G. F. & Moreira, B. M. ( 2006; ). Clonal composition of Staphylococcus aureus isolates at a Brazilian university hospital: identification of international circulating lineages. J Clin Microbiol 44, 1686–1691.[CrossRef]
    [Google Scholar]
  29. Wilson, D. J., Gonzalez, R. N. & Das, H. H. ( 1997; ). Bovine mastitis pathogens in New York and Pennsylvania: prevalence and effects on somatic cell count and milk production. J Dairy Sci 80, 2592–2598.[CrossRef]
    [Google Scholar]
  30. Zadoks, R., van Leeuwen, W., Barkema, H., Sampimon, O., Verbrugh, H., Schukken, Y. H. & van Belkum, A. ( 2000; ). Application of pulsed-field gel electrophoresis and binary typing as tools in veterinary clinical microbiology and molecular epidemiologic analysis of bovine and human Staphylococcus aureus isolates. J Clin Microbiol 38, 1931–1939.
    [Google Scholar]
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