1887

Abstract

In order to investigate the genetic relationships within isolated from marine mammals, two genome-based typing methods, variable number of tandem repeats (VNTR) typing and multilocus sequence analysis (MLSA), were applied to a selection of 74 marine mammal isolates. All isolates were examined by VNTR and data were compared with multilocus sequencing data from a subset of 48 of these. Marine mammal brucellae are distinct from classically recognized species by these methods and appear to correspond to three major genetic groups, which reflect distinct preferred hosts. One group contains isolates predominantly found in pinnipeds (seals) and corresponds to the previously proposed species ‘’. However, isolates corresponding to the previously proposed species ‘’ fall into two distinct groups that appear to have different preferred cetacean hosts (porpoises and dolphins). Furthermore, these two groups appear less closely related to each other than either group is to ‘’ isolates. The groups identified by VNTR typing and MLSA are completely congruent. The relevance of these findings to current proposals to recognize two species of marine mammal is discussed.

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2007-11-01
2019-10-14
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References

  1. Boschiroli, M. L., Foulongne, V. & O'Callaghan, D. ( 2001; ). Brucellosis: a worldwide zoonosis. Curr Opin Microbiol 4, 58–64.[CrossRef]
    [Google Scholar]
  2. Bourg, G., O'Callaghan, D. & Boschiroli, M. L. ( 2007; ). The genomic structure of Brucella strains isolated from marine mammals gives clues to evolutionary history within the genus. Vet Microbiol in presshttp://dx.doi.org
    [Google Scholar]
  3. Brew, S. D., Perrett, L. L., Stack, J. A., MacMillan, A. P. & Staunton, N. J. ( 1999; ). Human exposure to Brucella recovered from a sea mammal. Vet Rec 144, 483
    [Google Scholar]
  4. Clavareau, C., Wellemans, V., Walravens, K., Tryland, M., Verger, J. M., Grayon, M., Cloeckaert, A., Letesson, J. J. & Godfroid, J. ( 1998; ). Phenotypic and molecular characterization of a Brucella strain isolated from a minke whale (Balaenoptera acutorostrata). Microbiology 144, 3267–3273.[CrossRef]
    [Google Scholar]
  5. Cloeckaert, A., Verger, J. M., Grayon, M., Paquet, J. Y., Garin-Bastuji, B., Foster, G. & Godfroid, J. ( 2001; ). Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus. Microbes Infect 3, 729–738.[CrossRef]
    [Google Scholar]
  6. Cloeckaert, A., Grayon, M., Grepinet, O. & Boumedine, K. S. ( 2003; ). Classification of Brucella strains isolated from marine mammals by infrequent restriction site-PCR and development of specific PCR identification tests. Microbes Infect 5, 593–602.[CrossRef]
    [Google Scholar]
  7. Corbel, M. J. & Banai, M. ( 2005; ). Genus I. Brucella Meyer and Shaw 1920, 173AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 370–386. Edited by D. J. Brenner, N. R. Krieg & J. T. Staley. New York: Springer.
  8. Cutler, S. J., Whatmore, A. M. & Commander, N. J. ( 2005; ). Brucellosis – new aspects of an old disease. J Appl Microbiol 98, 1270–1281.[CrossRef]
    [Google Scholar]
  9. Davis, D. S. ( 1990; ). Brucellosis in wildlife. In Animal Brucellosis, pp. 321–34. Edited by K. Nielsen & J. R. Duncan. Boca Raton, FL: CRC Press.
  10. Ewalt, D. R., Payeur, J. B., Martin, B. M., Cummins, D. R. & Miller, W. G. ( 1994; ). Characteristics of a Brucella species from a bottlenose dolphin (Tursiops truncatus). J Vet Diagn Invest 6, 448–452.[CrossRef]
    [Google Scholar]
  11. Forbes, L. B., Nielsen, O., Measures, L. & Ewalt, D. R. ( 2000; ). Brucellosis in ringed seals and harp seals from Canada. J Wildl Dis 36, 595–598.[CrossRef]
    [Google Scholar]
  12. Foster, G., Jahans, K. L., Reid, R. J. & Ross, H. M. ( 1996; ). Isolation of Brucella species from cetaceans, seals and an otter. Vet Rec 138, 583–586.[CrossRef]
    [Google Scholar]
  13. Foster, G., MacMillan, A. P., Godfroid, J., Howie, F., Ross, H. M., Cloeckaert, A., Reid, R. J., Brew, S. & Patterson, I. A. ( 2002; ). A review of Brucella sp. infection of sea mammals with particular emphasis on isolates from Scotland. Vet Microbiol 90, 563–580.[CrossRef]
    [Google Scholar]
  14. Gandara, B., Merino, A. L., Rogel, M. A. & Martinez-Romero, E. ( 2001; ). Limited genetic diversity of Brucella spp. J Clin Microbiol 39, 235–240.[CrossRef]
    [Google Scholar]
  15. Garner, M. M., Lambourn, D. M., Jeffries, S. J., Hall, P. B., Rhyan, J. C., Ewalt, D. R., Polzin, L. M. & Cheville, N. F. ( 1997; ). Evidence of Brucella infection in Parafilaroides lungworms in a Pacific harbor seal (Phoca vitulina richardsi). J Vet Diagn Invest 9, 298–303.[CrossRef]
    [Google Scholar]
  16. Gee, J. E., De, B. K., Levett, P. N., Whitney, A. M., Novak, R. T. & Popovic, T. ( 2004; ). Use of 16S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates. J Clin Microbiol 42, 3649–3654.[CrossRef]
    [Google Scholar]
  17. Jahans, K. L., Foster, G. & Broughton, E. S. ( 1997; ). The characterisation of Brucella strains isolated from marine mammals. Vet Microbiol 57, 373–382.[CrossRef]
    [Google Scholar]
  18. Jensen, A. E., Cheville, N. F., Thoen, C. O., MacMillan, A. P. & Miller, W. G. ( 1999; ). Genomic fingerprinting and development of a dendrogram for Brucella spp. isolated from seals, porpoises, and dolphins. J Vet Diagn Invest 11, 152–157.[CrossRef]
    [Google Scholar]
  19. Le Fleche, P., Jacques, I., Grayon, M., Al Dahouk, S., Bouchon, P., Denoeud, F., Nockler, K., Neubauer, H., Guilloteau, L. A. & Vergnaud, G. ( 2006; ). Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. BMC Microbiol 6, 9 [CrossRef]
    [Google Scholar]
  20. McDonald, W. L., Jamaludin, R., Mackereth, G., Hansen, M., Humphrey, S., Short, P., Taylor, T., Swingler, J., Dawson, C. E. & other authors ( 2006; ). Characterization of a Brucella sp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand. J Clin Microbiol 44, 4363–4370.[CrossRef]
    [Google Scholar]
  21. Osterman, B. & Moriyón, I. ( 2006; ). International Committee on Systematics of Prokaryotes. Subcommittee on the Taxonomy of Brucella. Report of the Meeting, 17 September 2003, Pamplona, Spain. Int J Syst Evol Microbiol 56, 1173–1175.[CrossRef]
    [Google Scholar]
  22. Ross, H. M., Foster, G., Reid, R. J., Jahans, K. L. & MacMillan, A. P. ( 1994; ). Brucella species infection in sea-mammals. Vet Rec 134, 359
    [Google Scholar]
  23. Sohn, A. H., Probert, W. S., Glaser, C. A., Gupta, N., Bollen, A. W., Wong, J. D., Grace, E. M. & McDonald, W. C. ( 2003; ). Human neurobrucellosis with intracerebral granuloma caused by a marine mammal Brucella spp. Emerg Infect Dis 9, 485–488.[CrossRef]
    [Google Scholar]
  24. Verger, J. M., Grimont, F., Grimont, P. A. D. & Grayon, M. ( 1985; ). Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int J Syst Bacteriol 35, 292–295.[CrossRef]
    [Google Scholar]
  25. Whatmore, A. M., Murphy, T. J., Shankster, S., Young, E., Cutler, S. J. & Macmillan, A. P. ( 2005; ). Use of amplified fragment length polymorphism to identify and type Brucella isolates of medical and veterinary interest. J Clin Microbiol 43, 761–769.[CrossRef]
    [Google Scholar]
  26. Whatmore, A. M., Shankster, S. J., Perrett, L. L., Murphy, T. J., Brew, S. D., Thirlwall, R. E., Cutler, S. J. & Macmillan, A. P. ( 2006; ). Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J Clin Microbiol 44, 1982–1993.[CrossRef]
    [Google Scholar]
  27. Whatmore, A. M., Perrett, L. L. & Macmillan, A. P. ( 2007; ). Characterisation of the genetic diversity of Brucella by multilocus sequencing. BMC Microbiol 7, 34 [CrossRef]
    [Google Scholar]
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vol. , part 11, pp. 1512–1518

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