1887

Abstract

, a common cause of urinary tract infections, expresses iron-regulated outer-membrane proteins (OMPs) in response to iron restriction. It has been suggested that a 64 kDa OMP is involved in haemoprotein uptake and that this might have a role in pathogenesis. In order to confirm this hypothesis, this study generated a mutant strain (P7) that did not express the 64 kDa OMP, by insertion of the Tn transposon. The nucleotide sequence of the interrupted gene revealed that it corresponded to a haemin receptor precursor. Moreover, growth assays showed that the mutant was unable to grow using haemoglobin and haemin as unique iron sources. The authors also carried out growth and infectivity assays and demonstrated that P7 was not able to survive in an model and was less efficient than wild-type strain Pr 6515 in colonizing the urinary tract. These results confirmed that the 64 kDa iron-regulated OMP is a haem receptor that has an important role for survival and multiplication of these bacteria in the mammalian host and in the development of urinary tract infection.

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2007-12-01
2019-11-16
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