1887

Abstract

, a common cause of urinary tract infections, expresses iron-regulated outer-membrane proteins (OMPs) in response to iron restriction. It has been suggested that a 64 kDa OMP is involved in haemoprotein uptake and that this might have a role in pathogenesis. In order to confirm this hypothesis, this study generated a mutant strain (P7) that did not express the 64 kDa OMP, by insertion of the Tn transposon. The nucleotide sequence of the interrupted gene revealed that it corresponded to a haemin receptor precursor. Moreover, growth assays showed that the mutant was unable to grow using haemoglobin and haemin as unique iron sources. The authors also carried out growth and infectivity assays and demonstrated that P7 was not able to survive in an model and was less efficient than wild-type strain Pr 6515 in colonizing the urinary tract. These results confirmed that the 64 kDa iron-regulated OMP is a haem receptor that has an important role for survival and multiplication of these bacteria in the mammalian host and in the development of urinary tract infection.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.47320-0
2007-12-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/12/1600.html?itemId=/content/journal/jmm/10.1099/jmm.0.47320-0&mimeType=html&fmt=ahah

References

  1. Ahmed A. 1991; A comparison of intramolecular rearrangements promoted by transposons Tn5 and Tn10. Proc Biol Sci 244:1–9 [CrossRef]
    [Google Scholar]
  2. Ali A., Johnson J. A., Franco A. A., Metzger D. J., Connel T. D., Morris G., Sozhmannan S. 2000; Mutations in the extracellular protein secretion pathway genes ( eps ) interfere with rugose polysaccharide production in and motility of Vibrio cholerae . Infect Immun 68:1967–1974 [CrossRef]
    [Google Scholar]
  3. Braun V. 1995; Energy coupled transport and signal transduction through the Gram-negative outer membrane via TonB-ExbB-ExbD-dependent receptor proteins. FEMS Microbiol Rev 16:295–307 [CrossRef]
    [Google Scholar]
  4. Braun V. 2001; Iron uptake mechanisms and their regulation in pathogenic bacteria. Int J Med Microbiol 291:67–79 [CrossRef]
    [Google Scholar]
  5. Braun V., Braun M. 2002; Iron transport and signaling in Escherichia coli . FEBS Lett 529:78–85 [CrossRef]
    [Google Scholar]
  6. Bullock W. O., Fernández J. M., Short J. M. 1987; XL-1 Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β -galactosidase selection. Biotechniques 5:376–379
    [Google Scholar]
  7. Burall L. S., Harro J. M., Li X., Lockatell C. V., Himpsl S. D., Hebel J. R., Johnson D. E., Mobley H. L. T. 2004; Proteus mirabilis genes that contribute to pathogenesis of urinary tract infection: identification of 25 signature-tagged mutants attenuated at least 100-fold. Infect Immun 72:2922–2938 [CrossRef]
    [Google Scholar]
  8. Coker C., Poore C. A., Li X., Mobley H. L. T. 2000; Pathogenesis of Proteus mirabilis urinary tract infection. Microbes Infect 2:1497–1505 [CrossRef]
    [Google Scholar]
  9. Gallagher L. A., Manoil C. 2001; Pseudomonas aeruginosa PAO1 kills Caenorhabditis elegans by cyanide poisoning. J Bacteriol 183:6207–6214 [CrossRef]
    [Google Scholar]
  10. Hagberg L., Engberg I., Freter R., Lane J., Olling S., Svanborg-Eden C. 1983; Ascending unobstructed urinary tract infection in mice caused by pyelonephritogenic Escherichia coli of human origin. Infect Immun 40:273–283
    [Google Scholar]
  11. Litwin C. M., Calderwood S. B. 1993; Role of iron in regulation of virulence genes. Clin Microbiol Rev 6:137–149
    [Google Scholar]
  12. Llamas M. A., Bitter W. 2006; Iron gate: the translocation system. J Bacteriol 188:3172–3174 [CrossRef]
    [Google Scholar]
  13. Lukomski S., Serwecinska L., Rozalski A., Dziadek J., Staczek P., Jaworski A. 1991; Cell-free and cell-bound hemolytic activities of Proteus penneri determined by different Hly determinants. Can J Microbiol 37:419–424 [CrossRef]
    [Google Scholar]
  14. Manoil C., Beckwith J. 1985; Tn phoA : a transposon probe for protein export signal. Proc Natl Acad Sci U S A 82:8129–8133 [CrossRef]
    [Google Scholar]
  15. Maskell D. J., Morrisey P., Dougan G. 1988; Cloning and nucleotide sequence of the aroA gene of Bordetella pertussis . J Bacteriol 170:2467–2471
    [Google Scholar]
  16. Massad G., Zhao H., Mobley H. L. T. 1995; Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad . J Bacteriol 177:5878–5883
    [Google Scholar]
  17. Moayeri N., Collins C. M., O'Hanley P. 1991; Efficacy of a Proteus mirabilis outer membrane protein vaccine in preventing experimental pyelonephritis in a BALB/c mouse model. Infect Immun 59:3778–3786
    [Google Scholar]
  18. Mobley H. L. T., Chippendale G. R. 1990; Hemagglutinin, urease and hemolysin production by Proteus mirabilis from clinical sources. J Infect Dis 161:525–530 [CrossRef]
    [Google Scholar]
  19. Nagy G., Dobrindt U., Schneider G., Salam K. A., Hacker J., Emody L. 2002; Loss of regulatory protein RfaH attenuates virulence of uropathogenic Escherichia coli . Infect Immun 70:4406–4413 [CrossRef]
    [Google Scholar]
  20. Olczak T., Simpson W., Liu X., Genco C. A. 2005; Iron and heme utilization in Porphyromonas gingivalis . FEMS Microbiol Rev 29:119–144 [CrossRef]
    [Google Scholar]
  21. Piccini C. D., Barbé F. M., Legnani-Fajardo C. L. 1998; Identification of iron-regulated outer membrane proteins in uropathogenic Proteus mirabilis and its relationship with heme uptake. FEMS Microbiol Lett 166:243–248 [CrossRef]
    [Google Scholar]
  22. Quessy S., Dubreuil J. D., Jacques M., Malouin F., Higgins R. 1994; Increase of capsular material thickness following in vivo growth of virulent Streptococcus suis serotype 2 strains. FEMS Microbiol Lett 115:19–26 [CrossRef]
    [Google Scholar]
  23. Ròzalski A., Sidorxsyk Z., Kotelko K. 1997; Potential virulence factors of Proteus bacilli. Microbiol Mol Biol Rev 61:65–89
    [Google Scholar]
  24. Russo T. A., McFadden C. D., Carlino-MacDonald U. B., Beanan J. M., Barnard T. J., Johnson J. R. 2002; IroN functions as a siderophore receptor and is a urovirulence factor in an extraintestinal pathogenic isolate of Escherichia coli . Infect Immun 70:7156–7160 [CrossRef]
    [Google Scholar]
  25. Rutherford K., Parkhill J., Crook J., Horsnell T., Rice P., Rajandream M. A., Barrell B. 2000; Artemis: sequence visualization and annotation. Bioinformatics 16:944–945 [CrossRef]
    [Google Scholar]
  26. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Senior B. W. 1978; The special affinity of particular types of Proteus mirabilis for the urinary tract. J Med Microbiol 12:1–8
    [Google Scholar]
  28. Shand G. H., Anwar H., Kadurugamuya J., Brown M. R. W., Silverman S. H., Melling J. 1985; In vivo evidence that bacteria in urinary tract infection grow under iron-restricted conditions. Infect Immun 48:35–39
    [Google Scholar]
  29. Simon R., Priefer U., Puhler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1:784–791 [CrossRef]
    [Google Scholar]
  30. Stamm W. E., Martin S. M., Bennett J. V. 1977; Epidemiology of nosocomial infection due to Gram-negative bacilli: aspects relevant to development and use of vaccines. J Infect Dis 136:S151–S160 [CrossRef]
    [Google Scholar]
  31. Stojiljkovic I., Kumar V., Srinivasan N. 1999; Non-iron metalloporphyrins: potent antibacterial compounds that exploit haem/Hb uptake systems of pathogenic bacteria. Mol Microbiol 31:429–442 [CrossRef]
    [Google Scholar]
  32. Taylor R. K., Manoil C., Mekalanos J. J. 1989; Broad-host-range vectors for delivery of Tn phoA : use in genetic analysis of secreted virulence determinants of Vibrio cholerae . J Bacteriol 171:1870–1878
    [Google Scholar]
  33. Wandersman C., Stojiljkovic I. 2000; Bacterial heme sources: the role of heme, hemoprotein receptors and hemophores. Curr Opin Microbiol 3:215–220 [CrossRef]
    [Google Scholar]
  34. Warren J. W., Damron D., Tenney J. H., Hoopes J. M., Deforge B., Munice H. L. 1987; Fever, bacteremia, and death as complications of bacteriuria in women with long-term catheters. J Infect Dis 155:1151–1158 [CrossRef]
    [Google Scholar]
  35. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strain: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119 [CrossRef]
    [Google Scholar]
  36. Zunino P., Piccini C., Legnani-Fajardo C. 1994; Flagellate and non-flagellate Proteus mirabilis in the development of experimental urinary tract infection. Microb Pathog 16:379–385 [CrossRef]
    [Google Scholar]
  37. Zunino P., Piccini C., Legnani-Fajardo C. 1999; Growth, cellular differentiation and virulence factor expression by Proteus mirabilis in vitro and in vivo . J Med Microbiol 48:527–534 [CrossRef]
    [Google Scholar]
  38. Zunino P., Geymonat L., Allen A., Preston A., Sosa V., Maskell D. 2001; New aspects of the role of MR/P fimbriae in Proteus mirabilis urinary tract infection. FEMS Immunol Med Microbiol 31:113–120 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.47320-0
Loading
/content/journal/jmm/10.1099/jmm.0.47320-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error