1887

Abstract

Respiratory syncytial virus (RSV) infection is associated with secondary bacterial infections caused by nontypeable (NTHi) and . The pathogenesis of these complications is not completely understood; however, viral infection of respiratory epithelial cells promotes colonization by these bacteria. In the present study, RSV virions associated with NTHi and pneumococci in an inoculum-dependent manner in a fluid-phase binding assay. Adherence of NTHi and to epithelial cells transiently expressing RSV G glycoprotein was 2- and 2.2-fold higher, respectively, than adhesion to cells transfected with the vector alone ( <0.01). Furthermore, 4.6- and 6.2-fold larger numbers of NTHi and pneumococci bound to cells expressing a membrane-bound full-length RSV G protein than to cells expressing a truncated non-membrane-bound protein ( ≤0.005). Pre-incubating cells expressing membrane-bound G protein with blocking anti-RSV G antibodies reduced bacterial adherence by 78–84 % ( ≤0.005). These studies demonstrate that RSV G protein is a receptor for both NTHi and . Strategies to prevent this interaction may reduce the incidence of secondary bacterial complications of RSV infection.

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2007-09-01
2019-11-21
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