1887

Journal of Medical Microbiology

Volume 57, Issue 8

Agar dilution and agar screen with cefoxitin and oxacillin: what is known and what is unknown in detection of meticillin-resistant Free

Abstract

In this study we evaluated the performance of the oxacillin agar screen test, and agar dilution tests using cefoxitin and oxacillin antimicrobials, to detect meticillin resistance in isolates. The presence of the gene, detected by PCR, was used as the standard to which agar screen and agar dilution tests were compared. The best performance was obtained using the agar dilution test (99.4 % accuracy) with breakpoints of 4 μg ml for oxacillin and 8 μg ml for cefoxitin, and using the oxacillin agar screen test. Also, a strong correlation between MIC values of cefoxitin and oxacillin permits the use of either drug for detection of meticillin resistance.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CLSI, Clinical and Laboratory Standards Institute , MRSA, meticillin-resistant S. aureus and PBP, penicillin-binding protein
© SGM
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2008-08-01
2019-10-21
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Agar dilution and agar screen with cefoxitin and oxacillin: what is known and what is unknown in detection of meticillin-resistant Staphylococcus aureus
J. Med. Microbiol. 57, 954 (2008); https://doi.org/10.1099/jmm.0.46992-0
/content/journal/jmm/10.1099/jmm.0.46992-0
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