1887

Abstract

In this study we evaluated the performance of the oxacillin agar screen test, and agar dilution tests using cefoxitin and oxacillin antimicrobials, to detect meticillin resistance in isolates. The presence of the gene, detected by PCR, was used as the standard to which agar screen and agar dilution tests were compared. The best performance was obtained using the agar dilution test (99.4 % accuracy) with breakpoints of 4 μg ml for oxacillin and 8 μg ml for cefoxitin, and using the oxacillin agar screen test. Also, a strong correlation between MIC values of cefoxitin and oxacillin permits the use of either drug for detection of meticillin resistance.

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2008-08-01
2024-04-26
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References

  1. Amaral M. M., Coelho L. R., Flores R. P., Souza R. R., Silva-Carvalho M. C., Teixeira L. A., Ferreira-Carvalho B. T., Figueiredo A. M. 2005; The predominant variant of the Brazilian epidemic clonal complex of methicillin-resistant Staphylococcus aureus has an enhanced ability to produce biofilm and to adhere to and invade airway epithelial cells. J Infect Dis 192:801–810 [CrossRef]
    [Google Scholar]
  2. Cauwelier B., Gordts B., Descheemaecker P., Van Landuyt H. 2004; Evaluation of a disk diffusion method with cefoxitin (30 microg) for detection of methicillin-resistant Staphylococcus aureus . Eur J Clin Microbiol Infect Dis 23:389–392 [CrossRef]
    [Google Scholar]
  3. Chambers H. F. 2001; The changing epidemiology of Staphylococcus aureus? . Emerg Infect Dis 7:178–182 [CrossRef]
    [Google Scholar]
  4. CLSI 2006a Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically . Approved standard, CLSI document M7–A7 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  5. CLSI 2006b Performance Standards for Antimicrobial Susceptibility Testing . Sixteenth Informational Supplement Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  6. Fernandes C. J., Fernandes L. A., Collignon P. 2005; Cefoxitin resistance as a surrogate marker for the detection of methicillin-resistant Staphylococcus aureus . J Antimicrob Chemother 55:506–510 [CrossRef]
    [Google Scholar]
  7. Fuda C., Suvorov M., Vakulenko S. B., Mobashery S. 2004; The basis for resistance to beta-lactam antibiotics by penicillin-binding protein 2a of methicillin-resistant Staphylococcus aureus . J Biol Chem 279:40802–40806 [CrossRef]
    [Google Scholar]
  8. Pottumarthy S., Fritsche T. R., Jones R. N. 2005; Evaluation of alternative disk diffusion methods for detecting mec A-mediated oxacillin resistance in an international collection of staphylococci: validation report from the SENTRY Antimicrobial Surveillance Program. Diagn Microbiol Infect Dis 51:57–62 [CrossRef]
    [Google Scholar]
  9. Sharp S. E., Warren J. A., Thomson R. B. Jr 2005; Cefoxitin disk diffusion screen for confirmation of oxacillin-resistant Staphylococcus aureus isolates and utility in the clinical laboratory. Diagn Microbiol Infect Dis 51:69–71 [CrossRef]
    [Google Scholar]
  10. Skov R., Smyth R., Clausen M., Larsen A. R., Frimodt-Moller N., Olsson-Liljequist B., Kahlmeter G. 2003; Evaluation of a cefoxitin 30 microg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus . J Antimicrob Chemother 52:204–207 [CrossRef]
    [Google Scholar]
  11. Skov R., Smyth R., Larsen A. R., Bolmstrôm A., Karlsson A., Mills K., Frimodt-Moller N., Kahlmeter G. 2006; Phenotypic detection of methicillin resistance in Staphylococcus aureus by disk diffusion testing and Etest on Mueller-Hinton agar. J Clin Microbiol 44:4395–4399 [CrossRef]
    [Google Scholar]
  12. Swenson J. M., Tenover F. C. 2005; Results of disk diffusion testing with cefoxitin correlate with presence of mec A in Staphylococcus spp. J Clin Microbiol 43:3818–3823 [CrossRef]
    [Google Scholar]
  13. Teixeira L. A., Resende C. A., Ormonde L. R., Rosenbaum R., Figueiredo A. M., de Lencastre H., Tomasz A. 1995; Geographic spread of epidemic multiresistant Staphylococcus aureus clone in Brazil. J Clin Microbiol 33:2400–2404
    [Google Scholar]
  14. Vannuffel P., Laterre P. F., Bouyer M., Gigi J., Vandercam B., Reynaert M., Gala J. L. 1998; Rapid and specific molecular identification of methicillin-resistant Staphylococcus aureus in endotracheal aspirates from mechanically ventilated patients. J Clin Microbiol 36:2366–2368
    [Google Scholar]
  15. Velasco D., del Mar Tomas M., Cartelle M., Beceiro A., Perez A., Molina F., Moure R., Villanueva R., Bou G. 2005; Evaluation of different methods for detecting methicillin (oxacillin) resistance in Staphylococcus aureus . J Antimicrob Chemother 55:379–382 [CrossRef]
    [Google Scholar]
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