1887

Abstract

The aim of this study was to determine the extent of EMRSA-15 spread in hospitals in Singapore. Molecular analysis of 197 non-duplicate meticillin-resistant (MRSA) isolates collected from five acute care public hospitals in Singapore in May 2005 revealed that 66 (33.5 %) were EMRSA-15 while 121 (61.4 %) belonged to the endemic multidrug-resistant ST239 clone. Median and mode vancomycin MIC for both major clones of health-care-associated MRSA were relatively high at 2.0 μg ml. Subsequent laboratory surveillance data collected from the first half of 2006 confirmed increasing numbers of the EMRSA-15 clone – ranging from 25.0 to 66.1 % of all MRSA isolated in local hospitals – replacing the ST239 clone island-wide.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46950-0
2007-03-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/jmm/56/3/376.html?itemId=/content/journal/jmm/10.1099/jmm.0.46950-0&mimeType=html&fmt=ahah

References

  1. Clinical and Laboratory Standards Institute 2005 Performance Standards for Antimicrobial Susceptibility Testing , approved standard M100-S16 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  2. Enright M. C., Day N. P., Davies C. E., Peacock S. J., Spratt B. G. 2000; Multilocus sequence typing for characterization of methicillin-resistant and methicillin-sensitive clones of Staphylococcus aureus . J Clin Microbiol 38:1008–1015
    [Google Scholar]
  3. Hardy K. J., Oppenheim B. A., Gossain S., Gao F., Hawkey P. M. 2006; A study of the relationship between environmental contamination with methicillin-resistant Staphylococcus aureus (MRSA) and patients' acquisition of MRSA. Infect Control Hosp Epidemiol 27:127–132 [CrossRef]
    [Google Scholar]
  4. Hsu L. Y., Koh T. H., Singh K., Kang M. L., Kurup A., Tan B. H. 2005; Dissemination of multisusceptible methicillin-resistant Staphylococcus aureus in Singapore. J Clin Microbiol 43:2923–2925 [CrossRef]
    [Google Scholar]
  5. Hsu L. Y., Koh Y. L., Chlebicka N. L., Tan T. Y., Krishnan P., Lin R. T., Tee N., Barkham T., Koh T. H. 2006; Establishment of ST30 as the predominant clonal type among community-associated methicillin-resistant Staphylococcus aureus isolates in Singapore. J Clin Microbiol 44:1090–1093 [CrossRef]
    [Google Scholar]
  6. Lina G., Boutite F., Tristan A., Bes M., Etienne J., Vandenesch F. 2003; Bacterial competition for human nasal cavity colonization: role of staphylococcal agr alleles. Appl Environ Microbiol 69:18–23 [CrossRef]
    [Google Scholar]
  7. Maslow J., Slutsky A., Arbeit R. 1993; The application of pulsed field gel electrophoresis to molecular epidemiology. In Diagnostic Molecular Microbiology: Principles and Applications pp  563–572 Edited by Persing H., Smith T., Tenover F., White T. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Melter O., Aires de Sousa M., Laskafeldova K., Urbaskova P., Wunschova M., de Lencastre H. 2004; Delineation of the endemic and sporadic methicillin-resistant Staphylococcus aureus clones in a Czech hospital. Microb Drug Resist 10:218–223 [CrossRef]
    [Google Scholar]
  9. Okuma K., Iwakawa K., Turnidge J. D., Grubb W. B., Bell J. M., O'Brien F. G., Coombs G. W., Pearman J. W., Tenover F. C. other authors 2002; Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J Clin Microbiol 40:4289–4294 [CrossRef]
    [Google Scholar]
  10. O'Neill G. L., Murchan S., Gil-Setas A., Aucken H. M. 2001; Identification and characterization of phage variants of a strain of epidemic methicillin-resistant Staphylococcus aureus (EMRSA-15). J Clin Microbiol 39:1540–1548 [CrossRef]
    [Google Scholar]
  11. Pearman J. W., Coombs G. W., Grubb W. B., O'Brien F. 2001; A British epidemic strain of methicillin-resistant Staphylococcus aureus (UK EMRSA-15) in Western Australia. Med J Aust 174:662
    [Google Scholar]
  12. Sabat A., Krzyszton-Russjan J., Strzalka W., Filipek R., Kosowska K., Hryniewicz W., Travis J., Potempa J. 2003; New method for typing Staphylococcus aureus strains: multiple-locus variable-number tandem repeat analysis of polymorphism and genetic relationships of clinical isolates. J Clin Microbiol 41:1801–1804 [CrossRef]
    [Google Scholar]
  13. Smith J. M., Cook G. M. 2005; A decade of community MRSA in New Zealand. Epidemiol Infect 133:899–904 [CrossRef]
    [Google Scholar]
  14. Sutcliffe J., Grebe T., Tait-Kamradt A., Wondrack L. 1996; Detection of erythromycin-resistant determinants by PCR. Antimicrob Agents Chemother 40:2562–2566
    [Google Scholar]
  15. Udo E. E., Al-Sweih N., Noronha B. 2006; Characterisation of non-multiresistant methicillin-resistant Staphylococcus aureus (including EMRSA-15) in Kuwait Hospitals. Clin Microbiol Infect 12:262–269 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46950-0
Loading
/content/journal/jmm/10.1099/jmm.0.46950-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error