1887

Abstract

This study evaluated a novel peptide nucleic acid (PNA) probe targeting a region of the 23S rRNA gene of by fluorescence hybridization (FISH). Analytical performance was determined using 39 reference strains and other well-characterized strains of spp. and . The probe was found to be specific for the complex ( including and ). The diagnostic accuracy was evaluated with 264 blood cultures containing Gram-negative rods. Using conventional identification as the reference, performance specifications were as follows: sensitivity 98.8 %, specificity 99.5 %, positive predictive value 98.8 % and negative predictive value 99.5 %. Discrepancies were resolved by PNA FISH retest and phenotypic tests. In conclusion, the probe provided an accurate diagnosis within 3 h and may supplement other methods for direct identification of Gram-negative bacteria.

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2007-07-01
2020-04-07
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