1887

Abstract

The objective of this study was to develop and evaluate a rapid new method of identifying clinically relevant species. DNA extracted from different strains was used in a real-time PCR assay with SYBR Green and melting-curve analysis to identify species. Ten control strains and four bacterial strains of closely related genera were employed, and samples from 28 patients were used. All strains were identified correctly, and there was no cross-reaction with strains from genera closely related to . The sensitivity and specificity of the method were 90 and 100 %, respectively. This method can be used to rapidly detect species in culture, without cross-reaction with other closely related genera.

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2006-12-01
2019-09-18
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