1887

Abstract

The nitrate reductase assay (NRA) was used as an alternative method for detection of resistance to the first-line antituberculous drugs isoniazid, rifampicin, ethambutol and streptomycin. A total of 320 strains of were studied and the results compared with the proportion method (PM) on Löwenstein–Jensen medium. The mean time to obtain results was 10 days and the overall agreement between the NRA and PM was 98.8 %. The NRA was easy to perform and represents a useful tool for rapid and accurate determination of drug-resistant strains in low-resource countries.

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2006-07-01
2019-10-18
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References

  1. Abate, G., Mshana, R. N. & Miorner, H. ( 1998; ). Evaluation of a colorimetric assay based on 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) for rapid detection of rifampicin resistance in Mycobacterium tuberculosis. Int J Tuberc Lung Dis 2, 1011–1016.
    [Google Scholar]
  2. Ängeby, K. A., Klintz, L. & Hoffner, S. E. ( 2002; ). Rapid and inexpensive drug susceptibility testing of Mycobacterium tuberculosis with a nitrate reductase assay. J Clin Microbiol 40, 553–555.[CrossRef]
    [Google Scholar]
  3. Canetti, G., Fox, W., Khomenko, A. L., Mahler, H. T., Menon, N. K., Mitchison, D. A., Rist, N. & Smelev, N. A. ( 1969; ). Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programs. Bull World Health Organ 41, 21–43.
    [Google Scholar]
  4. Coban, A. Y., Birinci, A., Ekinci, B. & Durupinar, B. ( 2004; ). Drug susceptibility testing of Mycobacterium tuberculosis with nitrate reductase assay. Int J Antimicrob Agents 24, 304–306.[CrossRef]
    [Google Scholar]
  5. Laszlo, A., Rahman, M., Espinal, M., Raviglione, M. & WHO/IUATLD Network of Supranational Reference Laboratories ( 2002; ). Quality assurance programme for drug susceptibility testing of Mycobacterium tuberculosis in the WHO/IUATLD Supranational Reference Laboratory Network: five rounds of proficiency testing, 1994–1998. Int J Tuberc Lung Dis 6, 748–756.
    [Google Scholar]
  6. Lemus, D., Martin, A., Montoro, E., Portaels, F. & Palomino, J. C. ( 2004; ). Rapid alternative methods for detection of rifampicin resistance in Mycobacterium tuberculosis. J Antimicrob Chemother 54, 130–133.[CrossRef]
    [Google Scholar]
  7. Martin, A., Camacho, M., Portaels, F. & Palomino, J. C. ( 2003; ). Resazurin microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method. Antimicrob Agents Chemother 47, 3616–3619.[CrossRef]
    [Google Scholar]
  8. Martin, A., Montoro, E., Lemus, D. & 8 other authors ( 2005a; ). Multicenter evaluation of the nitrate reductase assay for drug resistance detection of Mycobacterium tuberculosis. J Microbiol Methods 63, 145–150.[CrossRef]
    [Google Scholar]
  9. Martin, A., Palomino, J. C. & Portaels, F. ( 2005b; ). Rapid detection of ofloxacin resistance in Mycobacterium tuberculosis by two low-cost methods: resazurin and nitrate reductase assay. J Clin Microbiol 43, 1612–1616.[CrossRef]
    [Google Scholar]
  10. Montoro, E., Lemus, D., Echemendía, M., Martin, A., Portaels, F. & Palomino, J. C. ( 2005; ). Comparative evaluation of the nitrate reduction assay, the MTT test, and the resazurin microtiter assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis. J Antimicrob Chemother 55, 500–505.[CrossRef]
    [Google Scholar]
  11. Musa, H. R., Ambroggi, M., Souto, A. & Ängeby, K. A. ( 2005; ). Drug susceptibility testing of Mycobacterium tuberculosis by a nitrate reductase assay applied directly on microscopy-positive sputum samples. J Clin Microbiol 43, 3159–3161.[CrossRef]
    [Google Scholar]
  12. Palomino, J. C. ( 2005; ). Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. Eur Respir J 26, 339–350.[CrossRef]
    [Google Scholar]
  13. Panaiotov, S. & Kantardjiev, T. ( 2002; ). Nitrate reductase assay for drug susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol 40, 3881–3882.[CrossRef]
    [Google Scholar]
  14. Ramachandran, R. & Paramasivan, C. N. ( 2003; ). What is new in the diagnosis of tuberculosis? Part II: techniques for drug susceptibility testing. Indian J Tuberc 50, 197–202.
    [Google Scholar]
  15. Simboli, N., Takiff, H., McNerney, R., López, B., Martin, A., Palomino, J. C., Barrera, L. & Ritacco, V. ( 2005; ). In-house phage amplification assay is a sound alternative for detecting rifampin-resistant Mycobacterium tuberculosis in low-resource settings. Antimicrob Agents Chemother 49, 425–427.[CrossRef]
    [Google Scholar]
  16. Solis, L. A., Shin, S. S., Han, L. L., Lanos, F., Stowell, M. & Sloutsky, A. ( 2005; ). Validation of a rapid method for detection of Mycobacterium tuberculosis resistance to isoniazid and rifampin in Lima, Peru. Int J Tuberc Lung Dis 9, 760–764.
    [Google Scholar]
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