1887

Abstract

In a search for novel bioactive cell surface structures of periodontal pathogens, it was found that sera from two patients with -associated infections reacted strongly at 17 kDa on immunoblots of outer-membrane protein (OMP) preparations. The 17 kDa antigen was also recognized by anti-CsgA ( curli major subunit) antibody. The 17 kDa protein was identified as peptidoglycan-associated lipoprotein (PAL; AaPAL) by two-dimensional immunoblotting and subsequent sequence analysis by mass spectrometry and bioinformatics tools. AaPAL was an OMP and a lipoprotein, and it had an OmpA-like domain. In a group of middle-aged subjects (=26), serum reactivity to AaPAL was associated with the presence of periodontitis but not with the oral detection of . Both human sera and rabbit antisera against three different types of antigens, the gel-purified AaPAL, whole-cell antigens, and CsgA, recognized putative PALs of oral haemophili in addition to AaPAL. The results demonstrated that the novel AaPAL is a conserved bacterial lipoprotein. It is expressed and is strongly immunoreactive. The antigenic cross-reactivity found between AaPAL and oral haemophili may enhance local and systemic immuno-inflammatory reactions in periodontitis.

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2006-07-01
2020-04-06
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