1887

Abstract

Among bacterial haemolysins, cholesterol-dependent cytolysins (CDCs) produced by various Gram-positive bacteria are known to exhibit a lethal activity in mice. In this study, recombinant CDCs of streptolysin O, pneumolysin, ivanolysin O, listeriolysin O and several listeriolysin O mutants were constructed and the relationship between cytolytic activity and the lethal activity of each recombinant protein in mice was examined. Specific activity for cytolysis was determined by a quantitative haemolytic assay. Each protein was injected intravenously into mice and the lethal activity was evaluated by measuring the time until death of the mice. The four full-length CDC proteins exhibited lethal activity and their activities were highly proportional to their cytolytic activities. Inhibition of haemolytic activity resulted in the loss of lethal activity and non-haemolytic mutants of listeriolysin O did not exhibit any lethal activity. These data clearly indicate that the lethal effect of CDC proteins is dependent on the cytolytic activity.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46333-0
2006-05-01
2020-10-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/5/505.html?itemId=/content/journal/jmm/10.1099/jmm.0.46333-0&mimeType=html&fmt=ahah

References

  1. Alouf J. E. 1999; Introduction to the family of the structurally related cholesterol-binding cytolysins (‘sulfhydryl-activated’ toxins. In The Comprehensive Sourcebook of Bacterial Protein Toxins pp  443–456 Edited by Alouf J. E., Freer J. H. London: Academic Press;
    [Google Scholar]
  2. Alouf J. E., Palmer M. W. 1999; Streptolysin O. In The Comprehensive Sourcebook of Bacterial Protein Toxins pp  457–475 Edited by Alouf J. E., Freer J. H. London: Academic Press;
    [Google Scholar]
  3. Baba H., Kawamura I., Kohda C., Nomura T., Ito Y., Kimoto T., Watanabe I., Ichiyama S., Mitsuyama M. 2001; Essential role of domain 4 of pneumolysin from Streptococcus pneumoniae in cytolytic activity as determined by truncated proteins. Biochem Biophys Res Commun 281:37–44 [CrossRef]
    [Google Scholar]
  4. Bernheimer A. W. 1976; Sulfhydryl activated toxins. In Mechanisms in Bacterial Toxinology pp  85–97 Edited by Bernheimer A. W. New York: Wiley;
    [Google Scholar]
  5. Billington S. J., Jost B. H., Songer J. G. 2000; Thiol-activated cytolysins: structure, function and role in pathogenesis. FEMS Microbiol Lett 182:197–205 [CrossRef]
    [Google Scholar]
  6. Geoffroy C., Gaillard J.-L., Alouf J. E., Berche P. 1987; Purification, characterization, and toxicity of the sulfhydryl-activated hemolysin listeriolysin O from Listeria monocytogenes . Infect Immun 55:1641–1646
    [Google Scholar]
  7. Halbert S. P. 1970; Streptolysin O. In Microbial Toxins vol III pp  69–98 Edited by Montie T. C., Kaids S., Ajl S. J. New York: Academic Press;
    [Google Scholar]
  8. Halbert S. P., Bircher R., Dahle E. 1961; The analysis of streptococcal infections. V. Cardiotoxicity of streptolysin O for rabbits in vivo. J Exp Med 113:759–784 [CrossRef]
    [Google Scholar]
  9. Halbert S. P., Bircher R., Dahle E. 1963; Studies on the mechanism of the lethal toxic action of streptolysin “O” and the protection by certain antiserotonin drugs. J Lab Clin Med 61:437–452
    [Google Scholar]
  10. Howard J. G., Wallace K. R. 1953a; The comparative resistances of the red blood cells of various species to haemolysis by streptolysin O and by saponin. Br J Exp Pathol 34:181–184
    [Google Scholar]
  11. Howard J. G., Wallace K. R. 1953b; The comparative resistances of rabbits, guinea pigs and mice to the lethal actions of streptolysin O and saponin. Br J Exp Pathol 34:185–190
    [Google Scholar]
  12. Jounblat R., Kadioglu A., Mitchell T. J., Andrew P. W. 2003; Pneumococcal behavior and host responses during bronchopneumonia are affected differently by the cytolytic and complement-activating activities of pneumolysin. Infect Immun 71:1813–1819 [CrossRef]
    [Google Scholar]
  13. Kingdon G. C., Sword C. P. 1970; Cardiotoxic and lethal effects of Listeria monocytogenes hemolysin. Infect Immun 1:373–379
    [Google Scholar]
  14. Kohda C., Kawamura I., Baba H., Nomura T., Ito Y., Kimoto T., Watanabe I., Mitsuyama M. 2002; Dissociated linkage of cytokine-inducing activity and cytotoxicity to different domains of listeriolysin O from Listeria monocytogenes . Infect Immun 70:1334–1341 [CrossRef]
    [Google Scholar]
  15. Limbago B., Penumalli V., Weinrick B., Scott J. R. 2000; Role of streptolysin O in a mouse model of invasive group A streptococcal disease. Infect Immun 68:6384–6390 [CrossRef]
    [Google Scholar]
  16. Michel E., Reich K. A., Favier R., Berche P., Cossart P. 1990; Attenuated mutants of the intracellular bacterium Listeria monocytogenes obtained by single amino acid substitutions in listeriolysin O. Mol Microbiol 4:2167–2178 [CrossRef]
    [Google Scholar]
  17. Owen R. H. G., Boulnois G. J., Andrew P. W., Mitchell T. J. 1994; A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae . FEMS Microbiol Lett 121:217–221 [CrossRef]
    [Google Scholar]
  18. Pfeffer K., Matsuyama T., Kündig T. M. & 7 other authors; 1993; Mice deficient for the 55 kd tumor necrosis factor receptor are resistant to endotoxic shock, yet succumb to L. monocytogenes infection. Cell 73:457–467 [CrossRef]
    [Google Scholar]
  19. Rothe J., Lesslauer W., Lötscher H. & 7 other authors; 1993; Mice lacking the tumor necrosis factor receptor 1 are resistant to TNF-mediated toxicity but highly susceptible to infection by Listeria monocytogenes . Nature 364:798–802 [CrossRef]
    [Google Scholar]
  20. Sekino-Suzuki N., Nakamura M., Mitsui K. I., Ohno-Iwashita Y. 1996; Contribution of individual tryptophan residues to the structure and activity of theta-toxin (perfringolysin O), a cholesterol-binding cytolysin. Eur J Biochem 241:941–947 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46333-0
Loading
/content/journal/jmm/10.1099/jmm.0.46333-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error