1887

Abstract

It was previously reported that two oligonucleotide primer sets (spn9802 and spn9828) for discriminating from pneumococcus-like oral streptococcal isolates using PCR had been developed. In this study, PCR amplification of the , , spn9802 and spn9828 genes was used to identify presumptive . Two genetic groups were identified by analysing sputum samples from 28 patients with community-acquired pneumonia: the -positive, -positive, spn9802-positive and spn9828-negative group, and the -positive, -positive, spn9802-positive and spn9828-positive group. Isolates of the former group were resistant to optochin, while those of the latter group showed susceptibility to optochin. The -positive, -positive, spn9802-negative and spn9828-negative isolates, and -positive, -positive, spn9802-negative and spn9828-positive isolates, were not detected in sputum from patients with pneumonia. Subsequently, a total of 92 saliva samples from healthy individuals was screened by PCR using these primer sets. The -positive, -positive, spn9802-positive and spn9828-negative group was identified more frequently in saliva from healthy children than in saliva from older healthy individuals and patients with pneumonia. The -positive, -positive, spn9802-positive and spn9828-positive group was found frequently in saliva from healthy children, and in saliva and sputum from patients with pneumonia. This study demonstrates a rapid, optimal screening method for the genotypic identification of presumptive by PCR using four genes highly specific for .

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2006-06-01
2019-10-14
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