1887

Abstract

A robust method for the identification of spp. based on direct sequencing of PCR-amplified partial sequences and comparison of these to a reference database of sequences is reported. A total of 53 isolates, representing 15 species, were identified and distinguished from phenotypically similar and strains. Pairwise sequence identities between spp. ranged from 71 to 92 %, with most between 71 and 79 %, making discrimination of these species obvious. The method described overcomes limitations of existing PCR-based methods, which require time-consuming and complex post-amplification steps such as the cloning of amplification products. The results of this study demonstrate the potential for use of the reference chaperonin sequence database, cpnDB, as a tool for identification of bacterial isolates based on sequences amplified with universal primers.

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2006-04-01
2020-09-26
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