1887

Abstract

Since 1986, penicillin non-susceptible pneumococci (PNSP) have been found in the Czech Republic. As documented by a nationwide study, the proportion of invasive strains with reduced susceptibility to penicillin has fluctuated around 5 % in the past decade. Although the level of resistance to penicillin remains stable, the contribution of different capsular serotypes among the PNSP population varies. Whereas serotype 19A was predominantly associated with penicillin resistance until 1997, serotype 9V became most common among PNSP strains in 1998. In a collection of PNSP strains (=225) isolated from 2000 to 2002, the most frequent serotype was 9V (=91, 40·4 %), followed by 19F (=30, 13·3 %) and 14 (=25, 11·5 %). PFGE and multilocus sequence typing were used to characterize a set of PNSP of the currently predominant serotypes 9V (=42), 14 (=15) and 19F (=14). The Spain-3 clone [sequence type (ST) 156] was responsible for a large proportion (100 % of serotype 9V strains, =42; 93·3 % of serotype 14 strains, =14) of the analysed strains. A representative of the Taiwan-14 clone (ST 236) was also recovered in the Czech Republic (a single isolate of serotype 19F). These findings confirm the spread of the major penicillin-resistant clones in the Czech Republic.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46270-0
2006-04-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/4/437.html?itemId=/content/journal/jmm/10.1099/jmm.0.46270-0&mimeType=html&fmt=ahah

References

  1. Baquero, F. ( 1995; ). Pneumococcal resistance to β-lactam antibiotics: a global geographic overview. Microb Drug Resist 1, 115–120.[CrossRef]
    [Google Scholar]
  2. Buxbaum, A., Forsthuber, S., Graninger, W. & Georgopoulos, A. ( 2004; ). Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae in Austria. J Antimicrob Chemother 54, 247–250.[CrossRef]
    [Google Scholar]
  3. Coffey, T. J., Dowson, C. G., Daniels, M., Zhou, J., Martin, C., Spratt, B. G. & Musser, J. M. ( 1991; ). Horizontal transfer of multiple penicillin-binding protein genes, and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae. Mol Microbiol 5, 2255–2260.[CrossRef]
    [Google Scholar]
  4. Coffey, T. J., Dowson, C. G., Daniels, M. & Spratt, B. G. ( 1995; ). Genetics and molecular biology of β-lactam-resistant pneumococci. Microb Drug Resist 1, 25–30.
    [Google Scholar]
  5. Coffey, T. J., Daniels, M., Enright, M. C. & Spratt, B. G. ( 1999; ). Serotype 14 variants of the Spanish penicillin-resistant serotype 9V clone of Streptococcus pneumoniae arose by large recombinational replacements of the cpsA–pbp1a region. Microbiology 145, 2023–2031.[CrossRef]
    [Google Scholar]
  6. European Antimicrobial Resistance Surveillance System ( 2004; ). European Antimicrobial Resistance Surveillance System annual report 2003. Ongoing surveillance of S. pneumoniae, S. aureus, E. coli, E. faecium, E. faecalis. Bilthoven, The Netherlands: RIVM.
  7. Enright, M. C. & Spratt, B. G. ( 1998; ). A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology 144, 3049–3060.[CrossRef]
    [Google Scholar]
  8. Enright, M. C., Fenoll, A., Griffiths, D. & Spratt, B. G. ( 1999; ). The three major Spanish clones of penicillin-resistant Streptococcus pneumoniae are the most common clones recovered in recent cases of meningitis in Spain. J Clin Microb 37, 3210–3216.
    [Google Scholar]
  9. Feil, E. J., Li, B. C., Aanensen, D. M., Hanage, W. P. & Spratt, B. G. ( 2004; ). eburst: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186, 1518–1530.[CrossRef]
    [Google Scholar]
  10. Gasc, A.-M., Geslin, P. & Sicard, A. M. ( 1995; ). Relatedness of penicillin-resistant Streptococcus pneumoniae serogroup 9 strains from France and Spain. Microbiology 141, 623–627.[CrossRef]
    [Google Scholar]
  11. Gertz, R. E., Jr, McEllistrem, C. M., Boxrud, D. J. & 9 other authors ( 2003; ). Clonal distribution of invasive pneumococcal isolates from children and selected adults in the United States prior to 7-valent conjugate vaccine introduction. J Clin Microbiol 41, 4194–4216.[CrossRef]
    [Google Scholar]
  12. Janečková, V., Toršová, V. & Chmelařová, E. ( 1989; ). The incidence of Streptococcus pneumoniae strains with resistance to penicillin. Cesk Epidemiol Mikrobiol Imunol 38, 342–347.
    [Google Scholar]
  13. McEllistrem, M. C., Adams, J., Mason, E. O. & Wald, E. R. ( 2003; ). Epidemiology of acute otitis media caused by Streptococcus pneumoniae before and after licensure of the 7-valent pneumococcal protein conjugate vaccine. J Infect Dis 188, 1679–1684.[CrossRef]
    [Google Scholar]
  14. McGee, L., McDougal, L., Zhou, J. & 8 other authors ( 2001; ). Nomenclature of major antimicrobial-resistant clones of Streptococcus pneumoniae defined by the pneumococcal molecular epidemiology network. J Clin Microbiol 39, 2565–2571.[CrossRef]
    [Google Scholar]
  15. Munoz, R., Coffey, T. J., Daniels, M. & 8 other authors ( 1991; ). Intercontinental spread of a multiresistant clone of serotype 23F Streptococcus pneumoniae. J Infect Dis 164, 302–306.[CrossRef]
    [Google Scholar]
  16. Obaro, S. & Adegbola, R. ( 2002; ). The pneumococcus: carriage, disease and conjugate vaccines. J Med Microbiol 51, 98–104.
    [Google Scholar]
  17. National Committee for Clinical Laboratory Standards ( 2000; ). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically (M7-A4), Approved standard (M100-S10), 5th edn. Wayne, PA: National Committee for Clinical Laboratory Standards.
  18. Porat, N., Arguedas, A., Spratt, B. G., Trefler, R., Brilla, E., Loaiza, C., Godoy, D., Bilek, N. & Dagan, R. ( 2004; ). Emergence of penicillin-nonsusceptible Streptococcus pneumoniae clones expressing serotypes not present in the antipneumococcal conjugate vaccine. J Infect Dis 190, 2154–2161.[CrossRef]
    [Google Scholar]
  19. Richter, S. S., Heilmann, K. P., Coffman, S. L., Huyng, H. K., Brueggemann, A. B., Pfaller, M. A. & Doern, G. V. ( 2002; ). The molecular epidemiology of penicillin-resistant Streptococcus pneumoniae in the United States, 1994–2000. Clin Infect Dis 34, 330–339.[CrossRef]
    [Google Scholar]
  20. Sa Figueiredo, A. M., Austrian, R., Urbaskova, P., Texeira, L. A. & Tomasz, A. ( 1995; ). Novel penicillin-resistant clones of Streptococcus pneumoniae in the Czech Republic and in Slovakia. Microb Drug Resistance 1, 71–78.[CrossRef]
    [Google Scholar]
  21. Sá-Leão, R., Tomasz, A. & de Lencastre, H. ( 2001; ). Multilocus sequence typing of Streptococcus pneumoniae clones with unusual drug resistance patterns: genetic backgrounds and relatedness to other epidemic clones. J Infect Dis 184, 1206–1210.[CrossRef]
    [Google Scholar]
  22. Serrano, I., Melo-Cristino, J., Carriço, J. A. & Ramirez, M. ( 2005; ). Characterization of the genetic lineages responsible for pneumococcal invasive disease in Portugal. J Clin Microbiol 43, 1706–1715.[CrossRef]
    [Google Scholar]
  23. Shi, Z. Y., Enright, M. C., Wilkinson, P., Griffiths, D. & Spratt, B. G. ( 1998; ). Identification of three major clones of multiply antibiotic-resistant Streptococcus pneumoniae in Taiwanese hospitals by multilocus sequence typing. J Clin Microbiol 36, 3514–3519.
    [Google Scholar]
  24. Soares, S., Kristinsson, K. G., Muser, J. M. & Tomasz, A. ( 1993; ). Evidence for the introduction of a multiresistant clone of serotype 6B Streptococcus pneumoniae from Spain to Iceland in the late 1980s. J Infect Dis 168, 158–163.[CrossRef]
    [Google Scholar]
  25. Song, J. H., Yang, J. W., Peck, K. R., Kim, S., Lee, N. Y., Jacobs, M. R., Applebaum, P. C. & Pai, C. H. ( 1997; ). Spread of multidrug-resistant Streptococcus pneumoniae in South Korea. Clin Infect Dis 25, 747–749.[CrossRef]
    [Google Scholar]
  26. Sorensen, U. B. ( 1993; ). Typing of pneumococci by using 12 pooled antisera. J Clin Microbiol 31, 2097–2100.
    [Google Scholar]
  27. Urbášková, P. & Motlová, J. ( 1999; ). Occurrence of Streptococcus pneumoniae resistant to penicillin and other antibiotics in the Czech Republic from 1996 to 1998. Klin Mikrobiol Inf Lek 5, 65–71.
    [Google Scholar]
  28. Urbášková, P., Motlová, J. & Žemličková, H. ( 2004; ). Antibiotic resistance in invasive pneumococci and their serotypes in the Czech Republic. Cas Lek Cesk 143, 178–183.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46270-0
Loading
/content/journal/jmm/10.1099/jmm.0.46270-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error