1887

Abstract

Missense alterations in genes and , which encode DNA repair enzymes, were sequenced from 30 clinical isolates of of Beijing genotype, mostly from patients with primary tuberculosis, to evaluate their contribution to anti-mycobacterial drug resistance. The mutation Arg to Gly at codon position 48 (CGG to GGG) of was found in 21 isolates; of these, 16 isolates also harboured the mutation Gly to Arg at position 58 (GGA to CGA) of . No statistically significant association was found between and mutations, and drug resistance. Furthermore, no mutations in or were found in any of 24 isolates resistant to multiple drugs, nor in 28 anti-mycobacterial drug-susceptible isolates of different genotypes. These data confirm that the polymorphism of genes is characteristic and unique to the Beijing phylogenetic lineage. The mutator phenotype does not appear to increase prevalence of drug resistance, but further studies are required to investigate the mutation rates of Beijing isolates in response to drug exposure.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46261-0
2006-05-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/5/599.html?itemId=/content/journal/jmm/10.1099/jmm.0.46261-0&mimeType=html&fmt=ahah

References

  1. Anh D. D., Borgdorff M. W., Van L. N., Lan N. T., van Gorkom T., Kremer K., van Soolingen D. 2000; Mycobacterium tuberculosis Beijing genotype emerging in Vietnam. Emerg Infect Dis 6:302–305 [CrossRef]
    [Google Scholar]
  2. Bifani P. J., Mathema B., Kurepina N. E., Kreiswirth B. N. 2002; Global dissemination of the Mycobacterium tuberculosis W-Beijing family strains. Trends Microbiol 10:45–52 [CrossRef]
    [Google Scholar]
  3. Brudey K., Driscoll J. R., Rigouts L. 63 other authors; 2006; Mycobacterium tuberculosis complex genetic diversity: mining the fourth international spoligotyping database (SpolDB4) for classification, population genetics and epidemiology. BMC Microbiol 6: (in press
    [Google Scholar]
  4. Filliol I., Driscoll J. R., van Soolingen D. & 44 other authors; 2003; Snapshot of moving and expanding clones of Mycobacterium tuberculosis and their global distribution assessed by spoligotyping in an international study. J Clin Microbiol 41:1963–1970 [CrossRef]
    [Google Scholar]
  5. Glynn J. R., Whiteley J., Bifani P. J., Kremer K., van Soolingen D. 2002; Worldwide occurrence of Beijing/W strains of Mycobacterium tuberculosis : a systematic review. Emerg Infect Dis 8:843–849 [CrossRef]
    [Google Scholar]
  6. Horst J. P., Wu T. H., Marinus M. G. 1999; Escherichia coli mutator genes. Trends Microbiol 7:29–36 [CrossRef]
    [Google Scholar]
  7. Kamerbeek J., Schouls L., Kolk A. & 8 other authors; 1997; Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol 35:907–914
    [Google Scholar]
  8. Lari N., Rindi L., Bonanni D., Tortoli E., Garzelli C. 2004; Beijing/W Mycobacterium tuberculosis in Italy. Emerg Infect Dis 10:958–959 [CrossRef]
    [Google Scholar]
  9. Lari N., Rindi L., Sola C., Bonanni D., Rastogi N., Tortoli E., Garzelli C. 2005; Genetic diversity, determined on the basis of katG 463 and gyrA 95 polymorphisms, spoligotyping, and IS 6110 typing, of Mycobacterium tuberculosis complex isolates from Italy. J Clin Microbiol 43:1617–1624 [CrossRef]
    [Google Scholar]
  10. Mizrahi V., Andersen S. J. 1998; DNA repair in Mycobacterium tuberculosis . What have we learnt from the genome sequence?. Mol Microbiol 29:1331–1339 [CrossRef]
    [Google Scholar]
  11. Mokrousov I., Otten T., Vyazovaya A. & 7 other authors; 2003; PCR-based methodology for detecting multidrug-resistant strains of Mycobacterium tuberculosis Beijing family circulating in Russia. Eur J Clin Microbiol Infect Dis 22:342–348 [CrossRef]
    [Google Scholar]
  12. Rad M. E., Bifani P., Martin C. & 8 other authors; 2003; Mutations in putative mutator genes of Mycobacterium tuberculosis strains of the W-Beijing family. Emerg Infect Dis 9:838–845 [CrossRef]
    [Google Scholar]
  13. Ramaswamy S., Musser J. M. 1998; Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis : 1998 update. Tuber Lung Dis 79:3–29 [CrossRef]
    [Google Scholar]
  14. Rindi L., Lari N., Bonanni D., Garzelli C. 2004; Detection of Mycobacterium tuberculosis principal genetic groups by a duplex real-time PCR targeting the katG and gyrA genes. J Microbiol Methods 59:283–287 [CrossRef]
    [Google Scholar]
  15. Sreevatsan S., Pan X., Stockbauer K., Connell N., Kreiswirth B., Whittam T., Musser J. 1997; Restricted structural gene polymorphism in the Mycobacterium tuberculosis complex indicates evolutionarily recent global dissemination. Proc Natl Acad Sci U S A 94:9869–9874 [CrossRef]
    [Google Scholar]
  16. van Embden J. D. A., Cave M. D., Crowford J. T. & 8 other authors; 1993; Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 31:406–409
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46261-0
Loading
/content/journal/jmm/10.1099/jmm.0.46261-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error