1887

Abstract

is one of the main causes of otitis media, sinusitis, meningitis, pneumonia and septicaemia in children, and the development of ampicillin resistance in is a cause of serious concern. The aim of the present study was to determine the prevalence of ampicillin resistance in colonizing the nasopharynx of school-going healthy North Indian children, and to compare the distribution of different biotypes and serotype b in this population. A total of 2400 school-going healthy children from 45 rural and 45 urban schools were enrolled. Nasopharyngeal swabs were collected from the children and cultured. was isolated from 1001 (41·7 %) of the 2400 nasopharyngeal swabs collected. All these isolates were biotyped and serotyped, and their antibiotic susceptibility tested. All eight biotypes were present in this population. The most prevalent biotypes were I (19·6 %), II (16·8 %) and III (25·0 %). Of the 1001 isolates, 316 (31·6 %) were type b and 685 (68·4 %) were non-type b , and 22·9 % were resistant to ampicillin, 41·9 % to chloramphenicol, 27·5 % to erythromycin and 67·3 % to co-trimoxazole. Of the 316 type b isolates, 44·0 % were ampicillin resistant, while only 13·1 % non-type b isolates were ampicillin resistant. Of the 229 ampicillin-resistant isolates, 196 (85·6 %) were positive for -lactamase; 93·4 % (214/229) were biotypes I, II and III, of which 49 % were biotype I, 27·9 % were type II and 16·6 % were type III. Most of the strains belonging to biotypes III–VIII were ampicillin sensitive. Ampicillin resistance is significantly more common in biotype I and serotype b than in other biotypes and serotypes.

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2006-02-01
2020-04-08
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References

  1. Adegbola R. A., Secka O., Lahai G. & 11 other authors; 2005; Elimination of Haemophilus influenzae type b (Hib) disease from the Gambia after the introduction of routine immunisation with a Hib conjugate vaccine: a prospective study. Lancet 366:144–150 [CrossRef]
    [Google Scholar]
  2. Albritton W. L., Penner S., Slaney L., Brunton J. 1978; Biochemical characteristics of Haemophilus influenzae in relationship to source of isolation and antibiotic resistance. J Clin Microbiol 7:519–523
    [Google Scholar]
  3. Alrawi A. M., Chern K. C., Cevallos V., Lietman T., Whitcher J. P., Margolis T. P., and Cunningham E. T. Jr 2002; Biotypes and serotypes of Haemophilus influenzae ocular isolates. Br J Ophthalmol 86:276–277 [CrossRef]
    [Google Scholar]
  4. Ayyagari A., Sharma P., Chakraborti A., Agarwal K. C. 1985; Isolation and detection of Haemophilus influenzae from patients of respiratory tract infections and their antibiotic susceptibility patterns in Chandigarh. Indian J Chest Dis Allied Sci 27:230–235
    [Google Scholar]
  5. Bakir M., Yagci A., Ulger N., Akbenlioglu C., Ilki A., Soyletir G., Basaran M. 2002; Pharyngeal colonization with Haemophilus influenzae type b among healthy Turkish infants and children. Pediatr Int 44:381–386 [CrossRef]
    [Google Scholar]
  6. Campos J., Chanyangam M., deGroot R., Smith A. L., Tenover F. C., Reig R. 1989; Genetic relatedness of antibiotic resistance determinants in multiply resistant Haemophilus influenzae . J Infect Dis 60:810–817
    [Google Scholar]
  7. Campos J., Román F., Pérez-Vázquez M., Aracil B., Oteo J., Cercenado E. & the Spanish Study Group for H. influenzae type f; (2003; Antibiotic resistance and clinical significance of Haemophilus influenzae type f. J Antimicrob Chemother 52:961–966 [CrossRef]
    [Google Scholar]
  8. Castillo-Febres O., Lopez G., Ortegal F., Estopinan M., Casanova-Escalona L., Sanchez-Naveda M., Decker M., Edwards K. M. 2005; Oropharyngeal carriers in Venezuelan children in two child care settings, vaccinated or not with Haemophilus influenzae type b tetanus toxoid conjugate vaccine (PRP-T). Invest Clin 46:15–24
    [Google Scholar]
  9. Dabernat H., Delmas C., Rich C., Livrelli V., Joly B. 1988; Characterization of Haemophilus influenzae isolated from patients with otitis media. Eur J Clin Microbiol Infect Dis 7:219–225 [CrossRef]
    [Google Scholar]
  10. Das B. K., Arora N. K., Mathur P., Ostwal P., Mandal S., Kabra S. K., Kapil A., Lalitha M. K., Thomas K. 2002; Nasopharyngeal carriage of Haemophilus influenzae . Indian J Pediatr 69:775–777 [CrossRef]
    [Google Scholar]
  11. Doern G. V. 1995; Susceptibility tests of fastidious bacteria. In Manual of Clinical Microbiology , 6th edn. pp  1342–1349 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Doern G. V., Brueggemann A. B., Pierce G., Holley H. P. Jr, Rauch A. 1997; Antibiotic resistance among clinical isolates of Haemophilus influenzae in the United States in 1994 and 1995 and detection of β -lactamase-positive strains resistant to amoxicillin-clavulanate: results of a national multicenter surveillance study. Antimicrob Agents Chemother 41:292–297
    [Google Scholar]
  13. Doern G. V., Jones R. N., Pfaller M. A., Kugler K. The SENTRY Participants Group 1999; Haemophilus influenzae and Moraxella catarrahalis from patients with community-acquired respiratory tract infections: antimicrobial susceptibility patterns from the SENTRY antimicrobial surveillance program (United States and Canada, 1997). Antimicrob Agents Chemother 43:385–389
    [Google Scholar]
  14. Gordon K. A., Biedenbach D. J., Jones R. N. 2003; Comparison of Streptococcus pneumoniae and Haemophilus influenzae susceptibilities from community-acquired respiratory tract infections and hospitalized patients with pneumonia: five-year results for the SENTRY antimicrobial surveillance program. Diagn Microbiol Infect Dis 46:285–289 [CrossRef]
    [Google Scholar]
  15. Granato P. A., Jurek E. A., Weiner L. B. 1983; Biotypes of Haemophilus influenzae : relationship to clinical source of isolation, serotype, and antibiotic susceptibility. Am J Clin Pathol 79:73–77
    [Google Scholar]
  16. Gratten M. 1983; Haemophilus influenzae , biotype VII. J Clin Microbiol 18:1015–1016
    [Google Scholar]
  17. Gratten M., Barker J., Shann F., Gerega G., Montgomery J., Kajoi M., Lupiwa T. 1985; The aetiology of purulent meningitis in highland children: a bacteriological study. P N G Med J 28:233–240
    [Google Scholar]
  18. Invasive Bacterial Infection Surveillance (IBIS) Group of the International Clinical Epidemiology Network; 2002; Are Haemophilus influenzae infections a significant problem in India? A prospective study and review. Clin Infect Dis 34:949–957 [CrossRef]
    [Google Scholar]
  19. Jain A., Kumar P., Awasthi S. 2005; High nasopharyngeal carriage of drug resistant Streptococcus pneumoniae and Haemophilus influenzae in North Indian schoolchildren. Trop Med Int Health 10:234–239 [CrossRef]
    [Google Scholar]
  20. Kilian M. 1976; A taxonomic study of the genus Haemophilus , with the proposal of a new species. J Gen Microbiol 93:9–62 [CrossRef]
    [Google Scholar]
  21. Kilian M., Sørensen I., Frederiksen W. 1979; Biochemical characteristics of 130 recent isolates from Haemophilus influenzae meningitis. J Clin Microbiol 9:409–412
    [Google Scholar]
  22. Kohno S., Hoban D. The PROTEKT Surveillance Study Group 2003; Comparative in vitro activity of telithromycin and β -lactam antimicrobials against bacterial pathogens from community-acquired respiratory tract infections: data from the first year of PROTEKT (1999-2000). J Chemother 15:335–341
    [Google Scholar]
  23. Levy J., Verhaegen G., Mol P. D., Couturier M., Dekegel D., Butzler J. P. 1993; Molecular characterization of resistance plasmids in epidemiologically unrelated strains of multiresistant Haemophilus influenzae . J Infect Dis 168:177–187 [CrossRef]
    [Google Scholar]
  24. Long S. S., Teter M. J., Gilligan P. H. 1983; Biotype of Haemophilus influenzae : correlation with virulence and ampicillin resistance. J Infect Dis 147:800–806 [CrossRef]
    [Google Scholar]
  25. Markowitz S. M. 1980; Isolation of an ampicillin-resistant, non- β -lactamase-producing strain of Haemophilus influenzae . Antimicrob Agents Chemother 17:80–83 [CrossRef]
    [Google Scholar]
  26. Mendelman P. M., Chaffin D. O., Stull T. L., Rubens C. E., Mack K. D., Smith A. L. 1984; Characterization of non- β -lactamase-mediated ampicillin resistance in Haemophilus influenzae . Antimicrob Agents Chemother 26:235–244 [CrossRef]
    [Google Scholar]
  27. Nag V. L., Ayyagari A., Venkatesh V., Ghar M., Yadav V., Prasad K. N. 2001; Drug resistant Haemophilus influenzae from respiratory tract infection in a tertiary care hospital in North India. Indian J Chest Dis Allied Sci 43:13–17
    [Google Scholar]
  28. National Committee for Clinical Laboratory Standards (NCCLS 2000 NCCLS Performance Standard for Antimicrobial Susceptibility Testing , document M 1000-S13 Wayne, PA: NCCLS;
    [Google Scholar]
  29. Olsen S. J., Dejsirilert S., Sangsuk L., Chunsutiwat S., Dowell S. E. 2005; Frequent Haemophilus influenzae type b colonization in rural Thailand. Pediatr Infect Dis J 24:739–742 [CrossRef]
    [Google Scholar]
  30. Patwari A. K., Bisht S., Srinivasan A., Deb M., Chattopadhya D. 1996; Aetiology of pneumonia in hospitalized children. J Trop Pediatr 42:15–20 [CrossRef]
    [Google Scholar]
  31. Pittman M. 1931; Variation and type specificity in the bacterial species Haemophilus influenzae . J Exp Med 53:471–472 [CrossRef]
    [Google Scholar]
  32. Raymond J., Armand-Lefevre L., Moulin F., Dabernat H., Commeau A., Gendrel D., Berche P. 2001; Nasopharyngeal colonization by Haemophilus influenzae in children living in an orphanage. Pediatr Infect Dis J 20:779–784 [CrossRef]
    [Google Scholar]
  33. Sharma A., Kaur R., Ganguly N. K., Singh P. D., Chakraborti A. 2002; Subtype distribution of Haemophilus influenzae isolates from North India. J Med Microbiol 51:399–404
    [Google Scholar]
  34. Sottnek F. O., Albritton W. L. 1984; Haemophilus influenzae biotype VIII. J Clin Microbiol 20:815–816
    [Google Scholar]
  35. Talon D., Leroy J., Dupont M. J., Bertrand X., Mermet F., Thouverez M., Estavoyer J. M. 2000; Antibiotic susceptibility and genotypic characterization of Haemophilus influenzae strains isolated from nasopharyngeal specimens from children in day-care centers in eastern France. Clin Microbiol Infect 6:519–524 [CrossRef]
    [Google Scholar]
  36. Teng L. J., Ho S. W., Lee G. S., Leh K. T. 1989; Serotypes, biotypes, and antimicrobial susceptibility of clinical isolates of Haemophilus influenzae . Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zai 22:68–74
    [Google Scholar]
  37. Uraz G., Simsek H., Celik B. 2000; β -lactamase activities and resistance to antibiotics of Haemophilus influenzae , H. parainfluenzae and H. aphrophilus strains identified in throat cultures from children. Drug Metabol Drug Interact 16:217–228
    [Google Scholar]
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