1887

Abstract

is one of the main causes of otitis media, sinusitis, meningitis, pneumonia and septicaemia in children, and the development of ampicillin resistance in is a cause of serious concern. The aim of the present study was to determine the prevalence of ampicillin resistance in colonizing the nasopharynx of school-going healthy North Indian children, and to compare the distribution of different biotypes and serotype b in this population. A total of 2400 school-going healthy children from 45 rural and 45 urban schools were enrolled. Nasopharyngeal swabs were collected from the children and cultured. was isolated from 1001 (41·7 %) of the 2400 nasopharyngeal swabs collected. All these isolates were biotyped and serotyped, and their antibiotic susceptibility tested. All eight biotypes were present in this population. The most prevalent biotypes were I (19·6 %), II (16·8 %) and III (25·0 %). Of the 1001 isolates, 316 (31·6 %) were type b and 685 (68·4 %) were non-type b , and 22·9 % were resistant to ampicillin, 41·9 % to chloramphenicol, 27·5 % to erythromycin and 67·3 % to co-trimoxazole. Of the 316 type b isolates, 44·0 % were ampicillin resistant, while only 13·1 % non-type b isolates were ampicillin resistant. Of the 229 ampicillin-resistant isolates, 196 (85·6 %) were positive for -lactamase; 93·4 % (214/229) were biotypes I, II and III, of which 49 % were biotype I, 27·9 % were type II and 16·6 % were type III. Most of the strains belonging to biotypes III–VIII were ampicillin sensitive. Ampicillin resistance is significantly more common in biotype I and serotype b than in other biotypes and serotypes.

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2006-02-01
2019-10-17
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