1887

Abstract

Both O157 : H7 and non-O157 : H7 strains are reported to cause haemolytic–uraemic syndrome (HUS). This study was carried out to explore the pathogenicity of O157 : H7 and non-O157 : H7 strains in experimentally inoculated dogs. Twenty 40-day-old dogs were randomly divided into four groups, and the groups (=5) were administrated orally with O157 : H7 strains HJ2001-1 (from a patient with serious haemorrhagic diarrhoea) and HZ2001-4 (from a domestic sheep kept in the house of a patient who died from diarrhoea and subsequent acute renal failure), HZ2001-9 (a non-O157 : H7 strain, from a 6-month-old child who died from diarrhoea and subsequent acute renal failure) or a control strain, EC8099. HJ2001-1 and HZ2001-4 caused slight diarrhoea, and the dogs recovered without any complications. However, HZ2001-9 resulted in watery diarrhoea accompanied with slightly bloody stools, followed by death on the fifth or sixth day. In the fatally infected experimental animals, necrotic lesions in the liver and bacterial embolism in the kidney were observed. The primary cause of death was microvascular thrombosis caused by the bacteria, leading to renal and multiple organ failure. Therefore, the non-O157 : H7 strain HZ2001-9 causes clinical signs and pathological lesions in dogs that are consistent with those in acute renal failure or HUS in humans.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.46239-0
2006-01-01
2019-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jmm/55/1/23.html?itemId=/content/journal/jmm/10.1099/jmm.0.46239-0&mimeType=html&fmt=ahah

References

  1. Barrow, P., Lovell, M. & Berchieri, A., Jr ( 1998; ). Use of lytic bacteriophage for control of experimental Escherichia coli septicemia and meningitis in chickens and calves. Clin Diagn Lab Immunol 5, 294–298.
    [Google Scholar]
  2. Bell, B. P., Goldoft, M., Griffin, P. M. & other authors ( 1994; ). A multistate outbreak of Escherichia coli O157 : H7-associated bloody diarrhea and hemolytic uremic syndrome from hamburgers. The Washington experience. JAMA 272, 1349–1353.[CrossRef]
    [Google Scholar]
  3. Beutin, L., Zimmermann, S. & Gleier, K. ( 1996; ). Rapid detection and isolation of Shiga-like toxin (verocytotoxin)-producing Escherichia coli by direct testing of individual enterohemolytic colonies from washed sheep blood agar plates in the VTEC-RPLA assay. J Clin Microbiol 34, 2812–2814.
    [Google Scholar]
  4. Blaser, M. J. ( 2004; ). Bacteria and diseases of unknown cause: hemolytic-uremic syndrome. J Infect Dis 189, 552–555.[CrossRef]
    [Google Scholar]
  5. Bonnet, R., Souweine, B., Gauthier, G., Rich, C., Livrelli, V., Sirot, J., Joly, B. & Forestier, C. ( 1998; ). Non-O157 : H7 Stx2-producing Escherichia coli strains associated with sporadic cases of hemolytic-uremic syndrome in adults. J Clin Microbiol 36, 1777–1780.
    [Google Scholar]
  6. Boyce, T. G., Swerdlow, D. L. & Griffin, P. M. ( 1995; ). Escherichia coli O157 : H7 and the hemolytic-uremic syndrome. N Engl J Med 333, 364–368.[CrossRef]
    [Google Scholar]
  7. Chandler, W. L., Jelacic, S., Boster, D. R., Ciol, M. A., Williams, G. D., Watkins, S. L., Igarashi, T. & Tarr, P. I. ( 2002; ). Prothrombotic coagulation abnormalities preceding the hemolytic-uremic syndrome. N Engl J Med 346, 23–32.[CrossRef]
    [Google Scholar]
  8. Chantrey, J., Chapman, P. S. & Patterson-Kane, J. C. ( 2002; ). Hemolytic-uremic syndrome in a dog. J Vet Med A 49, 470–472.[CrossRef]
    [Google Scholar]
  9. Dean-Nystrom, E. A., Bosworth, B. T., Cray, W. C., Jr & Moon, H. W. ( 1997; ). Pathogenicity of Escherichia coli O157 : H7 in the intestines of neonatal calves. Infect Immun 65, 1842–1848.
    [Google Scholar]
  10. Dean-Nystrom, E. A., Bosworth, B. T., Moon, H. W. & O'Brien, A. D. ( 1998; ). Escherichia coli O157 : H7 requires intimin for enteropathogenicity in calves. Infect Immun 66, 4560–4563.
    [Google Scholar]
  11. Dundas, S., Todd, W. T., Stewart, A. I., Murdoch, P. S., Chaudhuri, A. K. & Hutchinson, S. J. ( 2001; ). The central Scotland Escherichia coli O157 : H7 outbreak: risk factors for the hemolytic uremic syndrome and death among hospitalized patients. Clin Infect Dis 33, 923–931.[CrossRef]
    [Google Scholar]
  12. Eisen, J. A. ( 2001; ). Gastrogenomics. Nature 409, 463–466.[CrossRef]
    [Google Scholar]
  13. Goldfarb, S. & Adler, S. H. ( 2001; ). Acute Renal Failure: Pathophysiology and Treatment. Hospital Physician Nephrology Board Review Manual, vol. 4, no. 3. Wayne, PA: Turner White Communications.
  14. Gunzer, F., Hennig-Pauka, I., Waldmann, K. H., Sandhoff, R., Grone, H. J., Kreipe, H. H., Matussek, A. & Mengel, M. ( 2002; ). Gnotobiotic piglets develop thrombotic microangiopathy after oral infection with enterohemorrhagic Escherichia coli. Am J Clin Pathol 118, 364–375.[CrossRef]
    [Google Scholar]
  15. Hacker, J., Blum-Oehler, G., Muhldorfer, I. & Tschape, H. ( 1997; ). Pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution. Mol Microbiol 23, 1089–1097.[CrossRef]
    [Google Scholar]
  16. Heydermen, R. S., Soriani, M. & Hirst, T. R. ( 2001; ). Is immune cell activation the missing link in the pathogenesis of post-diarrhoeal HUS? Trends Microbiol 9, 262–266.[CrossRef]
    [Google Scholar]
  17. Holloway, S., Senior, D., Roth, L. & Tisher, C. C. ( 1993; ). Hemolytic uremic syndrome in dogs. J Vet Intern Med 7, 220–227.[CrossRef]
    [Google Scholar]
  18. Johnson, W. M., Lior, H. & Bezanson, G. S. ( 1983; ). Cytotoxic Escherichia coli O157 : H7 associated with haemorrhagic colitis in Canada. Lancet i, 76.
    [Google Scholar]
  19. Kaplan, B. S. & Drummond, K. N. ( 1978; ). The hemolytic-uremic syndrome is a syndrome. N Engl J Med 298, 964–966.[CrossRef]
    [Google Scholar]
  20. Karch, H., Janetzki-Mittmann, C., Aleksic, S. & Datz, M. ( 1996; ). Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture. J Clin Microbiol 34, 516–519.
    [Google Scholar]
  21. Karmali, M. A., Steele, B. T., Petric, M. & Lim, C. ( 1983a; ). Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet i, 619–620.
    [Google Scholar]
  22. Karmali, M. A., Petric, M., Lim, C., Fleming, P. C. & Steele, B. T. ( 1983b; ). Escherichia coli cytotoxin, haemolytic-uraemic syndrome, and haemorrhagic colitis. Lancet ii, 1299–1300.
    [Google Scholar]
  23. Karmali, M. A., Petric, M., Lim, C., Fleming, P. C., Arbus, G. S. & Lior, H. ( 1985; ). The association between idiopathic hemolytic uremic syndrome and infection by verotoxin-producing Escherichia coli. J Infect Dis 151, 775–782.[CrossRef]
    [Google Scholar]
  24. Ma, H., Wang, J. L., Wang, D. F., Xu, Q., Fu, B. N. & Tu, G. L. ( 2002; ). A comparison study between multiplex PCR and Vero cytotoxin assays for Shiga toxin-producing Escherichia coli. Haixia Yufang Yixue 8, 7–10 (in Chinese).
    [Google Scholar]
  25. O'Brien, A. O., Lively, T. A., Chen, M. E., Rothman, S. W. & Formal, S. B. ( 1983; ). Escherichia coli O157 : H7 strains associated with haemorrhagic colitis in the United States produce a Shigella dysenteriae 1 (SHIGA) like cytotoxin. Lancet i, 702.
    [Google Scholar]
  26. Paton, A. W. & Paton, J. C. ( 1998; ). Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J Clin Microbiol 36, 598–602.
    [Google Scholar]
  27. Perna, N. T., Plunkett, G., III, Burland, V. & 25 other authors ( 2001; ). Genome sequence of enterohaemorrhagic Escherichia coli O157 : H7. Nature 409, 529–533.[CrossRef]
    [Google Scholar]
  28. Su, C. & Brandt, L. J. ( 1995; ). Escherichia coli O157 : H7 infection in humans. Ann Intern Med 123, 698–714.[CrossRef]
    [Google Scholar]
  29. Thorpe, C. M. ( 2004; ). Shiga toxin-producing Escherichia coli infection. Clin Infect Dis 38, 1298–1303.[CrossRef]
    [Google Scholar]
  30. Wales, A. D., Pearson, G. R., Skuse, A. M., Roe, J. M., Hayes, C. M., Cookson, A. L. & Woodward, M. J. ( 2001; ). Attaching and effacing lesions caused by Escherichia coli O157 : H7 in experimentally inoculated neonatal lambs. J Med Microbiol 50, 752–758.
    [Google Scholar]
  31. Wang, J. Y. & Jin, H. J. ( 2002; ). A case report of bacillary dysentery with O157 : H7 mixed infection. Chin Med J 82, 1079 (in Chinese).
    [Google Scholar]
  32. Willshaw, G. A., Scotland, S. M., Smith, H. R., Cheasty, T., Thomas, A. & Rowe, B. ( 1994; ). Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157. J Clin Microbiol 32, 897–902.
    [Google Scholar]
  33. Yamada, S., Kai, A. & Kudoh, Y. ( 1994; ). Serodiagnosis by passive hemagglutination test and verotoxin enzyme-linked immunosorbent assay of toxin-producing Escherichia coli infections in patients with hemolytic-uremic syndrome. J Clin Microbiol 32, 955–959.
    [Google Scholar]
  34. Zhang, J., Xia, S. L., Chen, G., Chen, Z., Huang, P., Fu, B. & Tu, G. ( 2002; ). A study on acute renal failure after an outbreak of diarrhea in Suixian county, Henan province. Chin J Epidemiol 23, 105–107 (in Chinese).
    [Google Scholar]
  35. Zhou, T. & Zao, H. ( 2001; ). Escherichia coli. In Diagnosis and Illustration of Clinical Microbiology, pp. 101–104. Shanghai: Shanghai Science and Technology Publishing House (in Chinese).
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.46239-0
Loading
/content/journal/jmm/10.1099/jmm.0.46239-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error