1887

Abstract

In this study, the application of amplified rDNA restriction analysis (ARDRA) for characterizing toxinotype A strains isolated from individuals with botulism was evaluated. Ten restriction enzymes were tested for their suitability in ARDRA as a typing method and I was selected for the best outcome. Analysis of I restriction profiles of the amplified products divided isolates into three clusters. Non-toxigenic strains showed an ARDRA restriction pattern that was distinct from those observed for . The successful use of ARDRA for subdivision of in this study confirmed that this technique is a powerful method for typing of toxinotype A clonal diversity. In addition, it is rapid, sensitive and simple.

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2005-10-01
2024-11-09
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References

  1. Collins M. D., East A. K. 1998; Phylogeny and taxonomy of the food-borne pathogen Clostridium botulinum and its neurotoxins. J Appl Microbiol 84:5–17 [CrossRef]
    [Google Scholar]
  2. Franciosa G., Fenicia L., Pourshaban M., Aureli P. 1997; Recovery of a strain of Clostridium botulinum producing both neurotoxin A and neurotoxin B from canned macrobiotic food. Appl Environ Microbiol 63:1148–1150
    [Google Scholar]
  3. Franciosa G., Floridi F., Maugliani A., Aureli P. 2004; Differentiation of the gene clusters encoding botulinum neurotoxin type A complexes in Clostridium botulinum type A, Ab, and A(B) strains. Appl Environ Microbiol 70:7192–7199 [CrossRef]
    [Google Scholar]
  4. Hatheway C. 1995; Botulism: the present status of the disease. Curr Top Microbiol Immunol 19:55–75
    [Google Scholar]
  5. Hielm S., Björkroth J., Hyytiâ E., Korkeala H. 1998; Genomic analysis of Clostridium botulinum group II by pulsed field gel electrophoresis. Appl Environ Microbiol 64:703–708
    [Google Scholar]
  6. Hyytiâ E., Björkroth J., Hielm S., Korkeala H. 1999a; Characterization of Clostridium botulinum groups I and II by randomly amplified polymorphic DNA analysis and repetitive element sequence-based PCR. Int J Food Microbiol 48:179–189 [CrossRef]
    [Google Scholar]
  7. Hyytiâ E., Hielm S., Björkroth J., Korkeala H. 1999b; Biodiversity of Clostridium botulinum type E strains isolated from fish and fishery products. Appl Environ Microbiol 65:2057–2064
    [Google Scholar]
  8. Kurabachew M., Enger O., Sandaa R. A., Lemma E., Bjorvatn B. 2003; Amplified ribosomal DNA restriction analysis in the differentiation of related species of mycobacteria. J Microbiol Methods 55:83–90 [CrossRef]
    [Google Scholar]
  9. Lindostöm M. K., Jankola H. M., Hielm S., Hyytiâ E. K., Korkeala H. J. 1999; Identification of Clostridium botulinum with API 20 A, Rapid ID 32 A and RapID ANA II. FEMS Immunol Med Microbiol 24:267–274 [CrossRef]
    [Google Scholar]
  10. Moreira J. L., Mota R. M., Horta M. F., Teixeira S. M. R., Neumann E., Nicoli J. R., Nunes A. C. 2005; Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling. BMC Microbiol 23:5–15
    [Google Scholar]
  11. Nevas M., Lindström M., Hielm S., Björkroth K. J., Peck M. W., Korkeala H. 2005; Diversity of proteolytic Clostridium botulinum strains, determined by a pulsed-field gel electrophoresis approach. Appl Environ Microbiol 71:1311–1317 [CrossRef]
    [Google Scholar]
  12. Popoff M. R. 1995; Ecology of neurotoxigenic strains of clostridia. Curr Top Microbiol Immunol 195:1–29
    [Google Scholar]
  13. Pourshafie M., Saifie M., Shafie A., Vahdani P., Aslani M., Salemian J. 1998; An outbreak of food-borne botulism associated with contaminated locally made cheese in Iran. Scand J Infect Dis 30:92–94 [CrossRef]
    [Google Scholar]
  14. Sasaki E., Osawa R., Nishitani Y., Whiley R. A. 2004; ARDRA and RAPD analyses of human and animal isolates of Streptococcus gallolyticus . J Vet Med Sci 66:1467–1470 [CrossRef]
    [Google Scholar]
  15. Simpson L. L. 2004; Identification of the major steps in botulinum toxin action. Annu Rev Pharmacol Toxicol 44:167–193 [CrossRef]
    [Google Scholar]
  16. Skinner G. E., Gendel S. M., Fingerhut G. A., Solomon H. A., Ulaszek J. 2000; Differentiation between types and strains of Clostridium botulinum by riboprinting. J Food Prot 63:1347–1352
    [Google Scholar]
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