1887

Abstract

In this study, the application of amplified rDNA restriction analysis (ARDRA) for characterizing toxinotype A strains isolated from individuals with botulism was evaluated. Ten restriction enzymes were tested for their suitability in ARDRA as a typing method and I was selected for the best outcome. Analysis of I restriction profiles of the amplified products divided isolates into three clusters. Non-toxigenic strains showed an ARDRA restriction pattern that was distinct from those observed for . The successful use of ARDRA for subdivision of in this study confirmed that this technique is a powerful method for typing of toxinotype A clonal diversity. In addition, it is rapid, sensitive and simple.

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2005-10-01
2021-03-08
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