1887

Abstract

Although the micro-organisms forming the cutaneous microbiota are considered to play important roles in the modification and prevention of skin diseases, a comprehensive analysis of their composition has not yet been carried out because of difficulties in determining yet-to-be-cultured micro-organisms in the samples. Swab-scrubbed forehead skin samples of five healthy volunteers were analysed by profiling 16S rRNA genes, as well as by conventional culture methods, to provide a profile of the cutaneous microbiota that included yet-to-be-cultured bacteria from normal human skin. Cluster analyses of the 16S rRNA gene sequences indicated a marked increase in diversity compared with that derived from the culture methods. Nineteen previously recognized species and 13 novel phylotypes were obtained from the analysis of 416 clones. In addition to well-known bacteria such as and , phylotype A, the 16S rRNA gene of which is 97 % similar to that of , was detected in three of the five samples, in one of which it was the predominant clone. Culture-independent genetic profiling of 16S rRNA genes for detecting human cutaneous microbiota has allowed us to detect potentially novel components of the cutaneous microbiota in humans.

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2005-12-01
2019-11-21
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References

  1. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Bergogne-Bérézin, E. & Towner, K. J. ( 1996;). Acinetobacter spp.as nosocomial pathogens: microbiological, clinical, and epidemiological features. Clin Microbiol Rev 9, 148–165.
    [Google Scholar]
  3. Chiller, K., Selkin, B. A. & Murakawa, J. ( 2001;). Skin microflora and bacterial infections of the skin. J Invest Dermatol Symp Proc 6, 170–174.[CrossRef]
    [Google Scholar]
  4. Cole, J. R., Chai, B., Marsh, T. L. & 8 other authors ( 2003;). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  5. Evans, C. A. ( 1975;). Persistant individual differences in the bacterial flora of the skin of the forehead: numbers of propionibacteria. J Invest Dermatol 64, 42–46.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985;). Confidence limits of phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Frank, D. N., Spiegelman, G. B., Davis, W., Wagner, E., Lyons, E. & Pace, N. R. ( 2003;). Culture-independent molecular analysis of microbial constituents of the healthy human outer ear. J Clin Microbiol 41, 295–303.[CrossRef]
    [Google Scholar]
  8. Fredricks, D. N. ( 2001;). Microbial ecology of human skin in health and disease. J Invest Dermatol Symp Proc 6, 167–169.[CrossRef]
    [Google Scholar]
  9. Good, I. J. ( 1953;). The population frequencies of species and the estimation of population parameters. Biometrica 40, 237–264.[CrossRef]
    [Google Scholar]
  10. Hayashi, H., Sakamoto, M. & Benno, Y. ( 2002;). Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods. Microbiol Immunol 46, 535–548.[CrossRef]
    [Google Scholar]
  11. Hayashi, H., Sakamoto, M. & Benno, Y. ( 2004;). Evaluation of three different forward primers by terminal restriction fragment length polymorphism analysis for determination of fecal Bifidobacterium spp.in healthy subjects. Microbiol Immunol 48, 1–6.[CrossRef]
    [Google Scholar]
  12. Hill, K. E., Davies, C. E., Wilson, M. J., Stephens, P., Harding, K. G. & Thomas, D. W. ( 2003;). Molecular analysis of the microflora in chronic venous leg ulceration. J Med Microbiol 52, 365–369.[CrossRef]
    [Google Scholar]
  13. Kennedy, C. T. C. ( 1998;). The external ear. In Rook /Wilkinson /Ebling Textbook of Dermatology, 6th edn, pp. 3013. Edited by R. H. Champion, J. L. Burton & S. M. Breathnach. Oxford, UK: Blackwell.
  14. Lane, D. J. ( 1991;). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. New York: Wiley.
  15. Leyden, J. J. ( 2001;). The evolving role of Propionibacterium acnes in acne. Semin Cutan Med Surg 20, 139–143.[CrossRef]
    [Google Scholar]
  16. Leyden, J. J., Marples, R. R. & Kligman, A. M. ( 1974;). Staphylococcus aureus in the lesions in atopic dermatitis. Br J Dermatol 90, 525–530.[CrossRef]
    [Google Scholar]
  17. Marples, R. ( 1982;). The normal flora of different sites in the young adult. Curr Med Res Opin 7, 67–70.
    [Google Scholar]
  18. Marples, R. R. & McGinley, K. J. ( 1974;). Corynebacterium acnes and other anaerobic diphtheroids from human skin. J Med Microbiol 7, 349–357.[CrossRef]
    [Google Scholar]
  19. Nelson, K. E., Paulsen, I. T., Heidelberg, J. F. & Fraser, C. M. ( 2000;). Status of genome projects for nonpathogenic bacteria and archea. Nature Biotechnol 18, 1049–1054.[CrossRef]
    [Google Scholar]
  20. Norton, C. D. & LeChevallier, M. W. ( 2000;). A pilot study of bacteriological population changes through potable water treatment and distribution. Appl Environ Microbiol 66, 268–276.[CrossRef]
    [Google Scholar]
  21. Ogawa, T., Katsuoka, K., Kawano, K. & Nishiyama, S. ( 1994;). Comparative study of staphylococcal flora on the skin surface of atopic dermatitis patients and healthy subjects. J Dermatol 21, 453–460.[CrossRef]
    [Google Scholar]
  22. Pei, Z., Bini, E. J., Yang, L., Zhou, M., Francois, F. & Blaser, M. J. ( 2004;). Bacterial biota in the human distal esophagus. Proc Natl Acad Sci U S A 101, 4250–4255.[CrossRef]
    [Google Scholar]
  23. Quinn, J. P. ( 1998;). Clinical problems posed by multiresistant nonfermenting Gram-negative pathogens. Clin Infect Dis 27, S117–S124.[CrossRef]
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Seifert, H., Dijkshoorn, L., Gerner-Smidt, P., Pelzer, N., Tjernberg, I. & Vaneechoutte, M. ( 1997;). Distribution of Acinetobacter species on human skin: comparison of phenotypic and genotypic identification methods. J Clin Microbiol 35, 2819–2825.
    [Google Scholar]
  26. Selwyn, S. ( 1975;). Natural antibiosis among skin bacteria as a primary defence against infection. Br J Dermatol 93, 487–493.[CrossRef]
    [Google Scholar]
  27. Senol, E. ( 2004;). Stenotrophomonas maltophilia: the significance and role as a nosocomial pathogen. J Hosp Infect 57, 1–7.[CrossRef]
    [Google Scholar]
  28. Suau, A., Bonnet, R., Sutren, M., Godon, J.-J., Gibson, G. R., Collins, M. D. & Doré, J. ( 1999;). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65, 4799–4807.
    [Google Scholar]
  29. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  30. Vartivarian, S. E., Papadakis, K. A. & Anaissie, E. ( 1996;). Stenotrophomonas (Xanthomonas) maltophilia urinary tract infection. Arch Intern Med 156, 433–435.[CrossRef]
    [Google Scholar]
  31. von Wintzingerode, F., Göbel, U. B. & Stackebrandt, E. ( 1997;). Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis. FEMS Microbiol Rev 21, 213–229.[CrossRef]
    [Google Scholar]
  32. Ward, D. M., Weller, R. & Bateson, M. M. ( 1990;). 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community. Nature 345, 63–65.[CrossRef]
    [Google Scholar]
  33. Williams, M. M., Domingo, J. W. S., Meckes, M. C., Kelty, C. A. & Rochon, H. S. ( 2004;). Phylogenetic diversity of drinking water bacteria in a distribution system simulator. J Appl Microbiol 96, 954–964.[CrossRef]
    [Google Scholar]
  34. Zhou, X., Bent, S. J., Schneider, M. G., Davis, C. C., Islam, M. R. & Forney, L. J. ( 2004;). Characterization of vaginal microbial communities in adult healthy women using cultivation-independent methods. Microbiology 150, 2565–2573.[CrossRef]
    [Google Scholar]
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