1887

Journal of Medical Microbiology logo

Volume 54, Issue 11

Potential antimicrobial effects of human lactoferrin against oral infection with in mice Free

Abstract

is a food-borne pathogen that causes serious listeriosis in humans. Antimicrobial effects of human lactoferrin (hLF) against have been clearly demonstrated in studies. However, studies have not been reported yet. This study investigated whether the oral administration of hLF could inhibit oral infection of listeria in BALB/c mice. The MICs for several strains of were determined, and the most sensitive strain was used for the animal work. hLF was administered to BALB/c mice for 7 days, commencing 4 days before oral infection. The effect of hLF was determined by bacterial enumeration and histopathological analysis of the liver and spleen, which are well-known as the major targets of oral listeria infection in mice. In bacterial enumeration, hLF decreased the number of cells in the liver. Histopathologically, the size and frequency of necrotic foci in the liver samples decreased with hLF administration. However, these changes were not observed in the spleen samples. The mRNA levels of inflammatory cytokines, such as interleukin (IL)-1β, tumour necrosis factor (TNF)-α and interferon (IFN)-γ, decreased in the liver of mice receiving hLF. This study has shown that hLF decreases the hepatic colonization of , hepatic necrosis and expression of inflammatory cytokines. It revealed that perorally given hLF could mediate antimicrobial and anti-inflammatory activities remote from the gut (i.e. in the liver) of mice challenged with .

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45918-0
2005-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/11/JM541108.html?itemId=/content/journal/jmm/10.1099/jmm.0.45918-0&mimeType=html&fmt=ahah

References

  1. Bellamy W. M., Takase M., Yamauchi K., Wakabayashi H., Kawase K., Tomita M. 1992; Identification of the bactericidal domain of lactoferrin. Biochim Biophys Acta 1121130–136 [CrossRef]
     [Google Scholar]
  2. Bhimani R. S., Vendrov Y., Furmanski P. 1999; Influence of lactoferrin feeding and injection against systemic staphylococcal infections in mice. J Appl Microbiol 86:135–144 [CrossRef]
     [Google Scholar]
  3. Blanot S., Joly M. M., Vilde F., Jaubert F., Clement O., Frija G., Berche P. 1997; A gerbil model for rhombencephalitis due to Listeria monocytogenes . Microb Pathog 23:39–48 [CrossRef]
     [Google Scholar]
  4. Branen J., Davidson P. M. 2000; Activity of hydrolysed lactoferrin against foodborne pathogenic bacteria in growth media: the effect of EDTA. Lett Appl Microbiol 30:233–237 [CrossRef]
     [Google Scholar]
  5. Brock J. 1995; Lactoferrin: a multifunctional immunoregulatory protein?. Immunol Today 16:417–419 [CrossRef]
     [Google Scholar]
  6. Czuprynski C. J., Faith N. G. 2002; Sodium bicarbonate enhances the severity of infection in neutropenic mice orally inoculated with Listeria monocytogenes EGD. Clin Diagn Lab Immunol 9:477–481
     [Google Scholar]
  7. Czuprynski C. J., Faith N. G., Steinberg H. 2003; A/J mice are susceptible and C57BL/6 mice are resistant to Listeria monocytogenes infection by intragastric inoculation. Infect Immun 71:682–689 [CrossRef]
     [Google Scholar]
  8. Fleming D. W., Cochi S. L., MacDonald K. L. 7 other authors; 1985; Pasteurized milk as a vehicle of infection in an outbreak of listeriosis. N Engl J Med 312:404–407 [CrossRef]
     [Google Scholar]
  9. Hara K., Ikeda M., Saito S., Matsumoto S., Numata K., Kato N., Tanaka K., Sekihara H. 2002; Lactoferrin inhibits hepatitis B virus infection in cultured human hepatocytes. Hepatol Res 24:228–235 [CrossRef]
     [Google Scholar]
  10. Haversen L. A., Engberg I., Baltzer L., Dolphin G., Hanson L. A., Mattsby-Baltzer I. 2000; Human lactoferrin and peptides derived from a surface-exposed helical region reduce experimental Escherichia coli urinary tract infection in mice. Infect Immun 68:5816–5823 [CrossRef]
     [Google Scholar]
  11. Haversen L. A., Baltzer L., Dolphin G., Hanson L. A., Mattsby-Baltzer I. 2003; Anti-inflammatory activities of human lactoferrin in acute dextran sulphate-induced colitis in mice. Scand J Immunol 57:2–10 [CrossRef]
     [Google Scholar]
  12. Johnson V. J., Tsunoda M., Sharma R. P. 2000; Increased production of proinflammatory cytokines by murine macrophages following oral exposure to sodium selenite but not to seleno-l-methionine. Arch Environ Contam Toxicol 39:243–250 [CrossRef]
     [Google Scholar]
  13. Levay P. F., Viljoen M. 1995; Lactoferrin: a general review. Haematologica 80:252–267
     [Google Scholar]
  14. Lima M. F., Kierszenbaum F. 1985; Lactoferrin effects on phagocytic cell function.I. Increased uptake and killing of an intracellular parasite by murine macrophages and human monocytes. J Immunol 134:4176–4183
     [Google Scholar]
  15. Longhi C., Conte M. P., Penta M., Cossu A., Antonini G., Superti F., Seganti L. 2004; Lactoferricin influences early events of Listeria monocytogenes infection in THP-1 human macrophages. J Med Microbiol 53:87–91 [CrossRef]
     [Google Scholar]
  16. Mannering S. L., Zhong J., Cheers C. 2002; T-cell activation, proliferation and apoptosis in primary Listeria monocytogenes infection. Immunology 106:87–95 [CrossRef]
     [Google Scholar]
  17. Marco A. J., Prats N., Ramos J. A., Briones V., Blanco M., Dominguez L., Domingo M. 1992; A microbiological, histopathological and immunohistological study of the intragastric inoculation of Listeria monocytogenes in mice. J Comp Pathol 107:1–9 [CrossRef]
     [Google Scholar]
  18. Mattsby-Baltzer I., Roseanu A., Motas C., Elverfors J., Engberg I., Hanson L. A. 1996; Lactoferrin or a fragment thereof inhibits the endotoxin-induced interleukin-6 response in human monocytic cells. Pediatr Res 40:257–262 [CrossRef]
     [Google Scholar]
  19. McLauchlin J. 1990; Human listeriosis in Britain, 1967–85, a summary of 722 cases.1. Listeriosis during pregnancy and in the newborn. Epidemiol Infect 104:181–189 [CrossRef]
     [Google Scholar]
  20. Mead P. S., Slutsker L., Dietz V., McCaig L. F., Bresee J. S., Shapiro C., Griffin P. M., Tauxe R. V. 1999; Food-related illness and death in the United States. Emerg Infect Dis 5:607–625 [CrossRef]
     [Google Scholar]
  21. Menudier A., Bosiraud C., Nicolas J. A. 1991; Virulence of Listeria monocytogenes serovars and Listeria spp.in experimental infection of mice. J Food Protect 54:917–921
     [Google Scholar]
  22. Miyauchi H., Hashimoto S., Nakajima M., Shinoda I., Fukuwatari Y., Hayasawa H. 1998; Bovine lactoferrin stimulates the phagocytic activity of human neutrophils: identification of its active domain. Cell Immunol 187:34–37 [CrossRef]
     [Google Scholar]
  23. Murdock C. A., Matthews K. R. 2002; Antibacterial activity of pepsin-digested lactoferrin on foodborne pathogens in buffered broth systems and ultra-high temperature milk with EDTA. J Appl Microbiol 93:850–856 [CrossRef]
     [Google Scholar]
  24. Nibbering P. H., Ravensbergen E., Welling M. M., van Berkel L. A., van Berkel P. H., Pauwels E. K., Nuijens J. H. 2001; Human lactoferrin and peptides derived from its N terminus are highly effective against infections with antibiotic-resistant bacteria. Infect Immun 69:1469–1476 [CrossRef]
     [Google Scholar]
  25. Nomura T., Kawamura I., Tsuchiya K., Kohda C., Baba H., Ito Y., Kimoto T., Watanabe I., Mitsuyama M. 2002; Essential role of interleukin-12 (IL-12) and IL-18 for gamma interferon production induced by listeriolysin O in mouse spleen cells. Infect Immun 70:1049–1055 [CrossRef]
     [Google Scholar]
  26. Notermans S., Dufrenne J., Teunis P., Chackraborty T. 1998; Studies on the risk assessment of Listeria monocytogenes . J Food Protect 61:244–248
     [Google Scholar]
  27. Schwarze R., Bauermeister C. D., Ortel S., Wichmann G. 1989; Perinatal listeriosis in Dresden 1981–1986: clinical and microbiological findings in 18 cases. Infection 17:131–138 [CrossRef]
     [Google Scholar]
  28. Shin K., Yamauchi K., Teraguchi S., Hayasawa H., Tomita M., Otsuka Y., Yamazaki S. 1998; Antibacterial activity of bovine lactoferrin and its peptides against enterohemorrhagic Escherichia coli O157 : H7. Lett Appl Microbiol 26:407–411 [CrossRef]
     [Google Scholar]
  29. Siegman-Igra Y., Levin R., Weinberger M. & 20 other authors; 2002; Listeria monocytogenes infection in Israel and review of cases worldwide. Emerg Infect Dis 8:305–310 [CrossRef]
     [Google Scholar]
  30. Teberg A. J., Yonekura M. L., Salminen C., Pavlova Z. 1987; Clinical manifestations of epidemic neonatal listeriosis. Pediatr Infect Dis J 6:817–820 [CrossRef]
     [Google Scholar]
  31. Valenti P., Greco R., Pitari G., Rossi P., Aiello M., Melino G., Antonini G. 1999; Apoptosis of Caco-2 intestinal cells invaded by Listeria monocytogenes : protective effect of lactoferrin. Exp Cell Res 250:197–202 [CrossRef]
     [Google Scholar]
  32. Vorland L. H. 1999; Lactoferrin: a multifunctional glycoprotein. APMIS 107:971–981 [CrossRef]
     [Google Scholar]
  33. Welling M. M., Paulusma-Annema A., Balter H. S., Pauwels E. K., Nibbering P. H. 2000; Technetium-99m labelled antimicrobial peptides discriminate between bacterial infections and sterile inflammations. Eur J Nucl Med 27:292–301 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45918-0
Loading
Potential antimicrobial effects of human lactoferrin against oral infection with Listeria monocytogenes in mice
J. Med. Microbiol. 54, 1049 (2005); https://doi.org/10.1099/jmm.0.45918-0
/content/journal/jmm/10.1099/jmm.0.45918-0
/content/journal/jmm/10.1099/jmm.0.45918-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error