1887

Abstract

Enterohaemorrhagic O157 : H7 infections of man have been associated with consumption of unpasteurized goat's milk and direct contact with kid goats on petting farms, yet little is known about colonization of goats with this organism. To assess the contribution of flagella and intimin of O157 : H7 in colonization of the goat, 8-week-old conventionally reared goats were inoculated orally in separate experiments with 1×10 c.f.u. of a non-verotoxigenic strain of O157 : H7 (strain NCTC 12900 Nal), an aflagellate derivative (DMB1) and an intimin-deficient derivative (DMB2). At 24 h after inoculation, the three O157 : H7 strains were shed at approximately 5×10 c.f.u. (g faeces) from all animals. Significantly fewer intimin-deficient bacteria were shed only on days 2 ( = 0.003) and 4 ( = 0.014), whereas from day 7 to 29 there were no differences. Tissues from three animals inoculated with wild-type O157 : H7 strain NCTC 12900 Nal were sampled at 24, 48 and 96 h after inoculation and the organism was cultured from the large intestine of all three animals and from the duodenum and ileum of the animal examined at 96 h. Tissues were examined histologically but attaching-effacing (AE) lesions were not observed at any intestinal site of the animals examined at 24 or 48 h. However, the animal examined at 96 h, which had uniquely shed approximately 1×10 O157 : H7 (g faeces) for the preceding 3 days, showed a heavy, diffuse infection with cryptosporidia and abundant, multifocal AE lesions in the distal colon, rectum and at the recto-anal junction. These AE lesions were confirmed by immunohistochemistry to be associated with O157 : H7.

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2005-05-01
2019-11-19
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