1887

Abstract

Propolis exhibits antimicrobial, anti-inflammatory and other biological effects. The aim of this study was to evaluate the activity of 30 % ethanolic extract of Bulgarian propolis against 94 strains by three methods. By the agar-well diffusion method, only 13.8 % of the strains exhibited no inhibition by 30 μl propolis extract (containing 9 mg propolis) and all isolates were inhibited to some extent by 90 μl of the extract (27 mg propolis) per well. The mean diameters of growth inhibition by 30, 60 or 90 μl propolis extract or 30 μl 96 % ethanol per well were 16.8, 19.2, 27.5 and 8.3 mm, respectively. The propolis extract was more active than the ethanol ( < 0.001). With 90 μl propolis extract per well, 69.4 % of the strains exhibited large diameters of growth inhibition (⩾20 mm) versus 26.6 % with 30 μl per well ( < 0.001). With moist propolis discs, inhibition was detected in more strains (92.1 %) than with dried discs (78.2 %, < 0.05), with mean inhibitory diameters of 18.7 and 13.8 mm, respectively. By the agar dilution method, 100 and 300 μg propolis ml inhibited the growth of 57.1 % and 76.2 %, respectively, of the 21 strains tested. In conclusion, Bulgarian propolis had a strong and dose-dependent activity against most of the strains tested. Although the effect of propolis on is promising, further microbiological, pharmacological and clinical trials are required.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.45880-0
2005-05-01
2019-11-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/54/5/JM540512.html?itemId=/content/journal/jmm/10.1099/jmm.0.45880-0&mimeType=html&fmt=ahah

References

  1. Bankova, V. S., de Castro, S. L. & Marcucci, M. C. ( 2000;). Propolis: recent advances in chemistry and plant origin. Apidologie 31, 3–15.[CrossRef]
    [Google Scholar]
  2. Banskota, A. H., Tezuka, Y., Adnyana, I. K., Ishii, E., Midorikawa, K., Matsushige, K. & Kadota, S. ( 2001;). Hepatoprotective and anti-Helicobacter pylori activities of constituents from Brazilian propolis. Phytomedicine 8, 16–23.[CrossRef]
    [Google Scholar]
  3. Bonvehi, S. J. & Coll, V. F. ( 2000;). Study on propolis quality from China and Uruguay. Z Naturforsch [C] 55, 778–784.
    [Google Scholar]
  4. Burdock, G. A. ( 1998;). Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 36, 347–363.[CrossRef]
    [Google Scholar]
  5. Drago, L., Mombelli, B., De Vecchi, E., Fassina, M. C., Tocalli, L. & Gismondo, M. R. ( 2000;). In vitro antimicrobial activity of propolis dry extract. J Chemother 12, 390–395.[CrossRef]
    [Google Scholar]
  6. Grange, J. M. & Davey, R. W. ( 1990;). Antibacterial properties of propolis (bee glue). J R Soc Med 83, 159–160.
    [Google Scholar]
  7. Hegazi, A. G., Abd El Hady, F. K. & Abd Allah, F. A. ( 2000;). Chemical composition and antimicrobial activity of European propolis. Z Naturforsch [C] 55, 70–75.
    [Google Scholar]
  8. Krol, W., Scheller, S., Shani, J., Pietsz, G. & Czuba, Z. ( 1993;). Synergistic effect of ethanolic extract of propolis and antibiotics on the growth of Staphylococcus aureus. Arzneimittelforschung 43, 607–609.
    [Google Scholar]
  9. Mirzoeva, O. K., Grishanin, R. N. & Colder, P. C. ( 1997;). Antimicrobial action of propolis and some of its components: the effect on growth, membrane potential and motility of bacteria. Microbiol Res 152, 239–246.[CrossRef]
    [Google Scholar]
  10. Santos, F. A., Bastos, E. M., Uzeda, M., Carvalho, M. A., Farias, L. M., Moreira, E. S. & Braga, F. C. ( 2002;). Antibacterial activity of Brazilian propolis and fractions against oral anaerobic bacteria. J Ethnopharmacol 80, 1–7.[CrossRef]
    [Google Scholar]
  11. Stepanovic, S., Antic, N., Dakic, I. & Svabic-Vlahovic, M. ( 2003;). In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs. Microbiol Res 158, 353–357.[CrossRef]
    [Google Scholar]
  12. Velikova, M., Bankova, V., Sorkun, K., Houcine, S., Tsvetkova, I. & Kujumgiev, A. ( 2000;). Propolis from the Mediterranean region: chemical composition and antimicrobial activity. Z Naturforsch [C] 55, 790–793.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45880-0
Loading
/content/journal/jmm/10.1099/jmm.0.45880-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error