1887

Abstract

is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the pathogenicity of via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that encodes a minimum of three and five homologues that are involved in animal pathogenicity. Mass spectrometry analysis of culture supernatants revealed that wild-type and the mutants synthesized numerous signalling molecules, including -octanoyl-homoserine lactone, -decanoyl-homoserine lactone, -(3-hydroxyoctanoyl)--homoserine lactone, -(3-hydroxydecanoyl)--homoserine lactone and -(3-oxotetradecanoyl)--homoserine lactone, which was further confirmed by heterologous expression of the alleles in . Mutagenesis of the QS system increased the time to death and reduced organ colonization of aerosolized BALB/c mice. Further, intraperitoneal challenge of Syrian hamsters with the QS mutants resulted in a significant increase in the LD Using semi-quantitative plate assays, preliminary analysis suggests that QS does not affect lipase, protease and phospholipase C biosynthesis/secretion in . The findings of the investigation demonstrate that encodes multiple genes, and disruption of the QS alleles reduces animal pathogenicity, but does not affect exoproduct secretion.

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2004-11-01
2020-10-30
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