1887

Abstract

The human gastric pathogen expresses several putative outer-membrane proteins (OMPs), but the role of individual OMPs in colonization of the stomach by is still poorly understood. The role of four such OMPs (AlpA, AlpB, OipA and HopZ) in a guinea pig model of infection has been investigated. Single , , and isogenic mutants were constructed in the guinea pig-adapted, wild-type strain GP15. Guinea pigs were inoculated intragastrically with the wild-type strain, single mutants or a mixture of the wild-type and a single mutant in a 1 : 1 ratio. Three weeks after infection, could be isolated from stomach sections of all animals that were infected with the wild-type, the mutant or the mutant, but from only five of nine ( = 0.18) and one of seven ( = 0.02) animals that were infected with the or mutants, respectively. The and mutants colonized the majority of animals that were inoculated with the strain mixture, whereas and mutants could not be isolated from animals that were infected with the strain mixture ( < 0.01). Specific IgG antibody responses were observed in all animals that were infected with either the wild-type or a mutant, but IgG levels were lower in animals that were infected with either the or the mutants, compared to the wild-type strain ( < 0.05). In conclusion, absence of AlpA or AlpB is a serious disadvantage for colonization of the stomach by .

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2004-05-01
2019-09-20
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