1887

Abstract

In 2002, the Romanian National Reference Laboratory was invited to join the Strep-EURO project to study invasive infections. During 2003 and 2004, a total of 33 isolates recovered from invasive disease were received from eight Romanian counties. For comparison, 102 isolates from non-invasive disease, as well as a collection of 12 old invasive strains (isolated between 1967 and 1980) were included. All isolates were characterized by several methods: T and typing, presence of the fibronectin-binding protein F1 gene (), serum opacity factor (), and superantigen (SAg) genes (, , , , , , and ). The recent invasive isolates exhibited 19 types, of which 1, 81, 76, 49 and 78 covered 57 % of the strains. Furthermore, multilocus sequence typing analysis revealed nine new sequence types, corresponding to types 1, 12, 49, 81, 92, 100, 106 and 119. The non-invasive isolates comprised 24 different types with a predominance of 1 and 12; the old invasive strains were of eight types, of which four were unique for this group. All isolates harboured and ; was detected in all invasive strains, except for the 49 and 81 isolates. The majority of isolates from carriers, and patients with pharyngitis were positive, most of these (14 strains) being 12. High tetracycline resistance rates were noted among both invasive and control isolates (54 % and 35 %, respectively), whereas macrolide resistance rates were low (3 % and 5 %, respectively). Active and continuing surveillance is required to provide an accurate assessment of the disease burden and to provide epidemiological data on the character of isolates in Romania.

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2008-11-01
2019-10-22
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